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Jappleseed
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Registered: 05/06/10
Posts: 28
Last seen: 10 years, 8 months
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Mycobags inoculated with LC 7 days ago; no growth!
#14057749 - 03/02/11 11:42 PM (13 years, 3 days ago) |
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I searched the forum for info regarding the topic, and could not find any definitive answers as to what causes LC failure after inoculation into WBS.
The "Ralphster's Choice" package was purchased and LCs were made using Cyber's tek (light corn syrup). The LCs took 3 weeks to colonize to golf-ball size (shaken once every couple days). Half of them still haven't finished yet.
Doc's WBS tek was used, but medium-sized mycobags were used in place of jars. The bags cooled over 24 hrs and were inoculated with 4ccs of very concentrated LC (three different strains were used). The bags were lightly shaken to distribute the LC throughout the top of the bag. The bags have been kept just over 70 degrees in the dark. After almost 7 days there has been no sign of growth.
I am not a noob, but this is my first attempt at LC. I hear that LC->mycobags should be almost completed in 7-10 days, hence my reason for posting, as my bags have no growth at all.
Side note: one of the spore syringes from Ralphster's I had used a month ago on WBS jars, and I saw no growth. Strange since the mycelium grew in the LC, but I've seen no growth on grains yet...
Any help would be much appreciated. Thank you.
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i GrOw StUFF
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Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 5 months
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Re: Mycobags inoculated with LC 7 days ago; no growth! [Re: Jappleseed]
#14057938 - 03/03/11 12:35 AM (13 years, 3 days ago) |
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hmmm, how many quarts were in the bag? How long did you PC for? How wet did the WBS look after you PC'd?....With the LC method you used you can't be 100% sure that it wasn't contaminated....What are you using for a GE filter for the bag and how big is it?...a pic would help
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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Re: Mycobags inoculated with LC 7 days ago; no growth! [Re: i GrOw StUFF]
#14057945 - 03/03/11 12:36 AM (13 years, 3 days ago) |
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as well if you shot the LC into the center o fthe bag, it may be colonizing just in the middle where you can not see...
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Jappleseed
Stranger


Registered: 05/06/10
Posts: 28
Last seen: 10 years, 8 months
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Re: Mycobags inoculated with LC 7 days ago; no growth! [Re: i GrOw StUFF]
#14058009 - 03/03/11 12:57 AM (13 years, 2 days ago) |
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There were about 4 quarts of WBS in the bag. Since the seal on my PC broke, I actually used tyndallization to sterilize. I ran the PC for three consecutive nights at 1.5hrs each night. None of the grains burst since the bags were elevated out of the water. The WBS did not seem wet after I finished. The WBS looks moist, but there is no water in the bags. The bags look as they usually do when I PC... I've never had any contamination this way. The bags were purchased from mycofactory; the filters are about 1sq inch. In the case the LC was contaminated, is there a chance of no growth? I assumed mold would grow quickly if this was the case. I'll get some pics once I can get my hands on a camera. In the meantime, would it hurt to shake up the bags some more? Thanks for the fast replies!
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