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Hofmann1943
explorer



Registered: 03/05/09
Posts: 409
Loc: Forest
Last seen: 3 years, 9 months
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Contamination from same syringe (Trichoderma in Liquid and on agar)
#14055896 - 03/02/11 05:22 PM (12 years, 10 months ago) |
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I made a transfer from LC to Agar. And this is the result. This syringe is just to dirty, and it come from a respected dealer.

I mean look at this guy, sometimes at night i can see him, staring at me. Its really creepy.
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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klik339
I bomb atomically



Registered: 11/20/10
Posts: 99
Last seen: 3 years, 9 months
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Re: Contamination from same syringe (Trichoderma in Liquid and on agar) [Re: Hofmann1943]
#14057385 - 03/02/11 10:14 PM (12 years, 10 months ago) |
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damn man that sucks. try contacting the dealer and see if they will send a replacement.
-------------------- Everything I post is a lie. All content shared for educational purposes only.
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 4 months
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Re: Contamination from same syringe (Trichoderma in Liquid and on agar) [Re: klik339]
#14057858 - 03/03/11 12:11 AM (12 years, 10 months ago) |
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What do you mean a respected dealer? He sold you an LC?
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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Hofmann1943
explorer



Registered: 03/05/09
Posts: 409
Loc: Forest
Last seen: 3 years, 9 months
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Re: Contamination from same syringe (Trichoderma in Liquid and on agar) [Re: i GrOw StUFF]
#14058341 - 03/03/11 03:24 AM (12 years, 10 months ago) |
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not LC spores in syringe.
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 4 months
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Re: Contamination from same syringe (Trichoderma in Liquid and on agar) [Re: Hofmann1943]
#14060098 - 03/03/11 01:31 PM (12 years, 10 months ago) |
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Are you using a glove box or flow hood to inoculate your LC's and Petri dishes? Have you tried these spores on a BRF or grain jar?
Edited by i GrOw StUFF (03/03/11 01:40 PM)
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Hofmann1943
explorer



Registered: 03/05/09
Posts: 409
Loc: Forest
Last seen: 3 years, 9 months
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Re: Contamination from same syringe (Trichoderma in Liquid and on agar) [Re: i GrOw StUFF]
#14064462 - 03/04/11 02:30 AM (12 years, 10 months ago) |
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i'm using glove box and all the other cultures are ok, so i know it's not because of bad sterile tehnique. No only liquid and agar
-------------------- By Albert Hofmann : Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened. That very small dosage, the first dose of my experiments i planed, was very very strong.
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