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Amazon Shop: ½ Pint Jars, Paul Stamets

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OfflineCosmicTourist
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Registered: 02/25/03
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Dry spores
    #1405206 - 03/24/03 08:22 AM (14 years, 7 months ago)

My friend thinks that his pf jars are taking too long. He has no sign of colonization several weeks after inoculation. He thinks the problem is because he didn't let the spore syringe sit for very long and the spores from the print didn't get rehydrated. Are these jars still good? will the spores germinate in the jars eventually? what should he do?


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Offlineshirley knott
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Re: Dry spores [Re: CosmicTourist]
    #1405299 - 03/24/03 08:57 AM (14 years, 7 months ago)

several weeks, and nothing? :frown:

sorry man. dump the jars, clean them well, and start over. 


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buh


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OfflineCosmicTourist
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Re: Dry spores [Re: shirley knott]
    #1405791 - 03/24/03 11:55 AM (14 years, 7 months ago)

can't he still use the jars? Was the spore syringe the problem? Should he let the syringe sit and hydrate the spores? for how long should it sit?


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Invisiblecheesenoonions
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Re: Dry spores [Re: CosmicTourist]
    #1405796 - 03/24/03 11:57 AM (14 years, 7 months ago)

Stamets suggests 24 hours.... Use deionized water or bottled water also. If your water is crappy quality, that can affect your syringes.


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OfflineCosmicTourist
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Re: Dry spores [Re: cheesenoonions]
    #1405815 - 03/24/03 12:06 PM (14 years, 7 months ago)

Cool thanks. Should my dog start over with new jars, or should he reinoculate these with a proper syringe? These jars should eventualy colonize right? I've read that you can scrape a print directly into a jar, so I asume that so long as there are spores in there they should eventualy colonize right?


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Invisiblecheesenoonions
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Re: Dry spores [Re: CosmicTourist]
    #1405834 - 03/24/03 12:16 PM (14 years, 7 months ago)

Just start over. It's not worth the trouble in my opinion. Make some new syringes, and some new cakes. You can use those jars over again to hold new substrate. If you don't see anything in ten days (4-7 is ussually all it takes) and you have optimal temps, just start over.


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Amazon Shop: ½ Pint Jars, Paul Stamets

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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