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Offlinerandy420rhoads
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Sterilizing Hydrogen peroxide
    #14050941 - 03/01/11 08:30 PM (12 years, 10 months ago)

I have a quart jar of MYA agar from everythingmushrooms.com and i'm going to add Hydrogen peroxide to it. How can I do this, can I just fill a syring with 8 Mls cap it then boil it or will the render it useless? Is it already sterile? Do I just boil the syringe then fill it because that doesn't sound too sterile...


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OfflineRogerRabbitM
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Re: Sterilizing Hydrogen peroxide [Re: randy420rhoads]
    #14050978 - 03/01/11 08:37 PM (12 years, 10 months ago)

Use the MYA as is.  Peroxide is toxic to fungi.  Why are you wanting to use peroxide on agar?  It's far better to transfer healthy mycelium away to a clean dish than to try to nuke the contaminant.

A few years ago, we had guys mixing peroxide into their agar after it cooled, just before pouring. Since it slows mycelium down so much, and impedes mushroom spores from germinating, it fell out of favor pretty fast.  If you're having major trouble with bacteria infested clones, order antibiotic agar from the same place.
RR


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Offlinerandy420rhoads
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Re: Sterilizing Hydrogen peroxide [Re: RogerRabbit]
    #14051023 - 03/01/11 08:43 PM (12 years, 10 months ago)

Even though i'm preparing the agar( My first time trying :smile: ) for oysters I was refering to my book "Psilocybin the mushroom growers handbook" That specifies hydrogen peroxide added to agar was a great breakthrough in preventing contams from agar and other substrate as long as it was not used in multispore inoculation as it would kill spores.


Is this not the case?


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OfflineRogerRabbitM
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Re: Sterilizing Hydrogen peroxide [Re: randy420rhoads]
    #14051142 - 03/01/11 09:02 PM (12 years, 10 months ago)

You don't want to prevent contaminants on agar.  You want them to germinate and grow so you can move your mycelium away from them.  Peroxide won't kill all the contaminant spores, but rather delay their germination long enough for your wedge to make it to grains. 

Ya gotta be careful with the outdated info that's in a lot of the early literature.  Folks tried things and then wrote about what worked, often after only a time or two.  Today, we know far more about sterile procedure and isolation.

Remember, agar isn't for expanding mycelium.  That's what grains are for.  Agar is for isolating strains or isolating tissue away from contaminants.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlinerandy420rhoads
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Re: Sterilizing Hydrogen peroxide [Re: RogerRabbit]
    #14051195 - 03/01/11 09:10 PM (12 years, 10 months ago)

Ok i'd much prefer to leave out the peroxide anyway, but I believe the book was writtin in 2008 or 09, are you saying that's already outdated?


As far as wanting the contams to germinate to move the mycellium away from them... You are saying that after a tissue transfer it's ok for contams to pop up then cut out a healthy wedge from a combo mycellium/contam dish to a new one without spreading those contams?



(EDIT) I guess the original book was writtin in the 70's but this is the most up to date version with many new additions and info without alot of the old ones discouraged.


Edited by randy420rhoads (03/01/11 09:17 PM)


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