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SnarkyPuffs
Betcha can't eat just one!


Registered: 11/13/10
Posts: 112
Loc: Xibalba
Last seen: 12 years, 5 months
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Stalled?
#14049070 - 03/01/11 03:13 PM (12 years, 10 months ago) |
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Sorry for the poor quality camera phone shot but here's the deal: I have a half-dozen jars that were injected one month ago that have pretty-much ground to a halt a few weeks into the process. The jars are made up of two different batches of WBS but both sets were inoculated with the same Golden Teacher syringe.
What would cause two differently prepared WBS jar sets to fail/stall in the same exact manner? They seemed to be doing okay at first - nice spots/blobs of mycelium growing within the mix and then bam - they up and quit.
Thanks!
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 4 months
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Is that the only place the myc is forming? If your sure its stalled try to shake it to break it all up and distribute the myc everywhere, like you are supposed to do around 20$ colonization anyway....Did you already shake it?
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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SnarkyPuffs
Betcha can't eat just one!


Registered: 11/13/10
Posts: 112
Loc: Xibalba
Last seen: 12 years, 5 months
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Yeah... I shook the jars initially on inoculation and then a few weeks into the process. Most of the jars have isolated blobs of growth that are about the size of a nickle but they haven't budged in weeks.
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 4 months
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Now that I look at it again and the fact that you shook it up during the way, that doesn't appear to be mushroom mycelium in the jar. Kind of looks like trich in the first stage(the mean green)....That blotch looks very suspicious....Is there any signs of green? Did that spot grow fast and then stop? Do the other jars look like that?
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
Edited by i GrOw StUFF (03/01/11 03:36 PM)
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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1) your jars are way too full 2) looks like bacterial infection to me.
-------------------- You make it all possible. Doesn't it feel good?
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virus1824
Mr Mushroom



Registered: 09/25/05
Posts: 1,751
Loc: Europe
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Re: Stalled? [Re: Doc_T]
#14049295 - 03/01/11 03:51 PM (12 years, 10 months ago) |
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removed! sorry mistook for something that was not there
-------------------- A weekend wasted is never a wasted weekend
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henk600
Medical cannabis user



Registered: 07/08/10
Posts: 635
Loc: nederland
Last seen: 12 years, 3 months
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Re: Stalled? [Re: Doc_T]
#14049299 - 03/01/11 03:51 PM (12 years, 10 months ago) |
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I had the same strange white spot on some jars in the past, it dont look like myc. When i opened the jars before i threw them away it had an very nasty smell,not paint but a sort of rotten sugar smell what made me almost puke as nasty the smell was.
And 1 month? i think it is lost..........
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SnarkyPuffs
Betcha can't eat just one!


Registered: 11/13/10
Posts: 112
Loc: Xibalba
Last seen: 12 years, 5 months
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Thanks! I popped one open and enjoyed the delightful wafting stink of stank. Yup... two had no bad smell whatsoever and one actually seemed to be struggling along but as I already compromised the jars, I decided to to ditch them all and give it another whirl.
What's the primary cause of bacterial contamination? I have yet to get hit by molds in any projects but I seem to get regular visits from the bacterial fairy.
Edited by SnarkyPuffs (03/01/11 04:05 PM)
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 4 months
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If they are tyvek paper filters at the top I heard they are not ideal, I have never used them before.....You can try a real tyvek filter or microtape for the top...How did you sterilize? Did you use a still air box for inoculation?
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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SnarkyPuffs
Betcha can't eat just one!


Registered: 11/13/10
Posts: 112
Loc: Xibalba
Last seen: 12 years, 5 months
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Yeah, tyvek barrier... (I have micropore tape as well). Sterile technique is fairly tight... 90 mins of pressure cooking and then all work is done in a sanitized glovebox with gloves and treated sleeves.
I'm guessing that my seed mix was just too moist in the end.
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 4 months
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hmmm did you start the 90 minute timer when it reached 15 psi? How big are your holes in the disc on the top? But yeah, its not good if they are too wet....I like to lay my seed out on a towel for an hour or 2 before I load the jars....
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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