|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
bdzsmkr365247
π½ππͺπππ πππ¦πππ£π€



Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
|
Q about temp for incubation And MS injection N/E advice?
#14048389 - 03/01/11 12:56 PM (12 years, 10 months ago) |
|
|
Hello everyone at the shroomery message board! i nocked up 9 BRF style jars on feb 25 they are in a closet the temp is currently ranging from 60- 66 Degrees F i have not seen any signs of growth yet, i am normally patient when i grow,, i am worried that the spores may have died or something any suggestions/advice would be very appreciated thanks in advance everyone
Edited by bdzsmkr365247 (03/07/11 06:55 AM)
|
biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
|
|
those temps are going to make things be a bit slower then normal..
|
bdzsmkr365247
π½ππͺπππ πππ¦πππ£π€



Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
|
Re: Q about temp for incubation [Re: biologys]
#14048432 - 03/01/11 01:05 PM (12 years, 10 months ago) |
|
|
is there any way i might be able to SAFELY keep them warm? it seems like my entire house is in the 60's range of temp
|
hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
|
|
Use 60-66F for colonizing large substrates such as 5 quart bags of grain or large bulk substrates.
BRF cakes are more tolerant to the middle range of colonization temperatures than larger grain substrates. You could safely heat the spawning area up to 70-75F to increase colonization speed with little to no risk of causing contamination.
|
biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
|
|
place them above your fridge, it generates some heat
or you can place in a styrofoam with a warm jar of water...and heat the jar of water as needed...
|
bdzsmkr365247
π½ππͺπππ πππ¦πππ£π€



Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
|
Re: Q about temp for incubation [Re: biologys]
#14048465 - 03/01/11 01:11 PM (12 years, 10 months ago) |
|
|
would hanging a reflector lamp above the shoe box the jars are in be a SAFE idea? also if i do use the lamp, should it be on a timer?
|
bestscreenname
Stranger

Registered: 02/28/11
Posts: 34
Loc: Charleston, South Carolin...
Last seen: 12 years, 15 days
|
|
Quote:
bdzsmkr365247 said: would hanging a reflector lamp above the shoe box the jars are in be a SAFE idea? also if i do use the lamp, should it be on a timer?
im wondering this same thing. if it would provide enough heat or not. i actually just signed up here to ask this. i only need a slight raise in temp for optimal conditions for my incubation station. im thinking it would work somewhat, just have to keep a safe distance between the chamber and the light and keep a close eye on the temp.
|
El Capitan Fresh
Mycophyte


Registered: 03/01/11
Posts: 3
Last seen: 12 years, 10 months
|
|
Quote:
bdzsmkr365247 said: is there any way i might be able to SAFELY keep them warm? it seems like my entire house is in the 60's range of temp 
I am dealing with this exact situation in my house. It is Winter, around 40 outside and about 60 in my old, radiator heated domicile.
Putting the box up high will help because it is probably five degrees warmer near your ceiling because heat rises. I personally just drape some lit christmas tree lights on my incubating shoebox. This helps with a few degrees and is a good choice because you wont need a timer.
Within days I've had kickass colonization in my 60ish degree house keeping my fungus at 76ish.
Good luck man.
|
Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
|
|
Best, safest, most reliable jar warming method I've found: put them in a styrofoam cooler with a jar of warm water. You can zap the water once a day or as needed. Maintain temps no higher than about 75-78F for best results.
Zero danger of overheating or catching fire while providing gentle warmth for germination.
-------------------- You make it all possible. Doesn't it feel good?
|
godfann1
ME


Registered: 02/17/04
Posts: 345
Loc: somewhere this side of oz
|
Re: Q about temp for incubation [Re: Doc_T]
#14050602 - 03/01/11 07:25 PM (12 years, 10 months ago) |
|
|
Ive always put my jars inside a rubbermade container on one side and put a heating pad under the other side of the container. Seemed to work really good for me.
-------------------- Everything in my posts are a figment of your imagination. Its all fake so lay off the mushies you're seeing things that are not there.
|
bdzsmkr365247
π½ππͺπππ πππ¦πππ£π€



Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
|
Re: Q about temp for incubation [Re: godfann1]
#14052922 - 03/02/11 05:48 AM (12 years, 10 months ago) |
|
|
weel i have found a place in my house the jars are at a constant 68 degrees F ,, is this a good temp for colonization? there is still no lil fuzzy spots or signs of colonization. any additional advice would be great thanks everyone for what you have given already!
|
godfann1
ME


Registered: 02/17/04
Posts: 345
Loc: somewhere this side of oz
|
|
I see that alot of people on here now are sayin temps on the 70s. Im just now getting back into this but when i did it before i always kept mine in the low to mid 80s and the jars col. really fast. yours may grow at that temp but prolly pretty slow.
-------------------- Everything in my posts are a figment of your imagination. Its all fake so lay off the mushies you're seeing things that are not there.
|
bdzsmkr365247
π½ππͺπππ πππ¦πππ£π€



Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
|
Re: Q about temp for incubation [Re: godfann1]
#14058482 - 03/03/11 05:36 AM (12 years, 10 months ago) |
|
|
yeah still no signs of col. the spores whre a lil old i am not sure if that has anything to do with it?
|
hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
|
|
Old spores will definitely be finicky about germinating. Get laboratory grade rattle snake venom to crack the stubborn exterior cellular wall of the aged spores to get them to germinate or, visit the Marketplace or order new genetics from a known and trusted vendor.
|
Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
|
|
7-14 days to see growth from spores.
-------------------- You make it all possible. Doesn't it feel good?
|
dietrich
Zauberpilz-Esser



Registered: 07/06/10
Posts: 319
Last seen: 5 years, 8 months
|
Re: Q about temp for incubation [Re: Doc_T]
#14059185 - 03/03/11 10:37 AM (12 years, 10 months ago) |
|
|
Keep us updated and let us know how colonization goes in the higher temp. I'm curious as well, because I'm waiting for mine to germinate, and the house is only around 69 degrees.
-------------------- Ich heiΓe Dietrich der Zauberpilz-Esser und bringe ich Frieden und Liebe fΓΌr alle.
|
bdzsmkr365247
π½ππͺπππ πππ¦πππ£π€



Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
|
Re: Q about temp for incubation [Re: dietrich]
#14060540 - 03/03/11 02:43 PM (12 years, 10 months ago) |
|
|
well, i don't own any rattle snakes or i would try the venom thing, today is day six, i am going to give these little guys another week or two before i start over, i have faith in B+
although i did impulsively buy more spores yesterday! i am so stoked to noc up more jars,
if any one has any experience with (orisa india) i would love to have some feedback, from what i understand they produce some large caps,
i only have the equipment for BRF cakes but i plan on spawning the BRF cakes to a mono tub for the the orisa
if Ne one cares i gathered up Treasure Coast *Personal Fav.!! Cambodian and Honduras
Hawks are amazing birds....
|
bdzsmkr365247
π½ππͺπππ πππ¦πππ£π€



Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
|
Re: Q about temp for incubation *DELETED* [Re: bdzsmkr365247]
#14060694 - 03/03/11 03:11 PM (12 years, 10 months ago) |
|
|
Post deleted by bdzsmkr365247Reason for deletion: useless
|
bdzsmkr365247
π½ππͺπππ πππ¦πππ£π€



Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
|
|
well,,, this morning while i was have my coffee, i decided to take a check on my jars.
i have 9 in incubation labeled (A,B,C,D,E,F,G,H,i) Oddly enough
Jar A and Jar i are showing some signs of growth, each jar has one cute little fuzzz ball each i am so happy to see that they are starting to kick off!
oh yeah,, the temp is....
68F
|
Primal Call
Earth Mage



Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 3 months
|
|
I'm by no means experienced, but I do a lot of research and intend to think rationally regardless... IMO just leave them be... you have nothing to lose by giving them longer than "normal" germination times. I mean, something like a month+ after inoculation is reasonable to question, but trashing them after 13 days seems, well, impatient.
from all my research, patience is one of the highest priorities when cultivating mushies.
good luck! I hope it works out!
|
bdzsmkr365247
π½ππͺπππ πππ¦πππ£π€



Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
|
Re: Q about temp for incubation [Re: Primal Call]
#14065964 - 03/04/11 12:00 PM (12 years, 10 months ago) |
|
|
egh i am patient,, mostly.. i have done this before so i am very aware that this is a hobby of EXTREME patients!!
i am going to give them more time as previously stated!
temp is Currently 66F
i am impatient when i have to wait for my spores to get delivered though HAHA
|
RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
|
|
Quote:
i am very aware that this is a hobby of EXTREME patients!!
That's what doctors in acute care hospitals do, but it's not a hobby.  RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
bdzsmkr365247
π½ππͺπππ πππ¦πππ£π€



Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
|
Re: Q about temp for incubation [Re: RogerRabbit]
#14070246 - 03/05/11 10:28 AM (12 years, 10 months ago) |
|
|
HAHAhaha SENIOR RABBIT!! thanks for posting on my thread!! i consider you al knowing...
current temp: 68
jar (i) has two fuzzyballs now jar (a) still only has one and the rest are still up in the air..
|
bdzsmkr365247
π½ππͺπππ πππ¦πππ£π€



Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
|
|
well,, the temp is at a steady 70-72F i am so stoked, two jars are starting to show col. but the rest are still nothing, i just thought i would keep y'all updated
|
bdzsmkr365247
π½ππͺπππ πππ¦πππ£π€



Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
|
|
I did a multi spore injection o the jars, the needle got clogged a few times, every time i injected i squirted some H2o out, is it possible that some jars may not have gotten spores??
the reason i ask this is cause two 1/2 pints are showing signs of colonization and the rest are not doing a thing
|
jmello



Registered: 01/27/08
Posts: 264
Last seen: 1 day, 11 hours
|
|
Quote:
bdzsmkr365247 said: I did a multi spore injection o the jars, the needle got clogged a few times, every time i injected i squirted some H2o out, is it possible that some jars may not have gotten spores??
the reason i ask this is cause two 1/2 pints are showing signs of colonization and the rest are not doing a thing 
Its not likely cuz if you got a syringe from a vendor there are thousands of spores in a syringe...even if you forget to shake before innoc (ive done it plenty) your chances are still high for success. I have gotten an old syringe that took an exceptionally long time to show any signs of growth, longer than times your dealing with. I would do g2g with the jars u have colonizing now, seems like the myc is a little less finicky with what it wants to eat
--------------------
|
jakedorset
Stranger
Registered: 02/19/11
Posts: 9
Last seen: 12 years, 9 months
|
Re: Q about temp for incubation [Re: jmello]
#14081609 - 03/07/11 03:04 PM (12 years, 10 months ago) |
|
|
i'm sure you got some spores in there. remember, you only need 2, and there's billions in that syringe.
|
bdzsmkr365247
π½ππͺπππ πππ¦πππ£π€



Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
|
Re: Q about temp for incubation [Re: jakedorset]
#14083326 - 03/07/11 07:49 PM (12 years, 10 months ago) |
|
|
the jars i knocked up are BRF not grain, i do not have pressure cooker to sterilize grains, could i do a BRF to grain any suggestions? i am planning on doing a bulk dubtub and some monos.
|
bdzsmkr365247
π½ππͺπππ πππ¦πππ£π€



Registered: 05/18/10
Posts: 301
Loc: ΘΆΙ¦Ι ΚΗΙ’Ι¨Ζ Ι’ΗΚΙΙΥΌ
|
|
well,,,, three of the 1/2 pints are colonizing i am starting to think the rest are goners .. i knocked up 12 pint size jars . 6 orisa and 6 TC!
the temps are ranging from67-70.. unfortunatelyyy.....
|
Primal Call
Earth Mage



Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 3 months
|
|
those temps are fine... sure, on the low side, but this is a good thing when considering contams thriving in 80F+ situations.
again, you say you are patient, but it's only been just over 2 weeks since your inoculation. sure, this is not ideal, but it can happen... I recently had a jar of CR take just under 4 weeks simply to germinate. even after it did, it colonized SUPER slowly on the grain at temps around 72F, so I did a G2G and now it's charging through 5qt jars. maybe it was the moisture content, the temps, the phase of the moon, or the position of the 6th ring on saturn... but I'm sure glad I didn't give up on it.
do what you will, but giving up hope on them when they aren't yet contamed (at least not obviously) seems wasteful IMO 
on BRF to grain, it probably isn't a good idea without a PC. you'd be better off spawning BRF to pasteurized bulk
good luck with it. sending vibes your way
|
|