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Offlinethomas_knobs
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Registered: 09/23/10
Posts: 330
Last seen: 1 year, 10 months
Weak genetics?
    #14047616 - 03/01/11 09:42 AM (12 years, 10 months ago)

I recently made an LC by stabbing a small mushroom stem (the only viable remaining living specimen that I had at the time). The culture has grown nicely and is ready to use for grain inoculation, but i'm a little concerned as to the genetics. Should I be worried about producing a strain with weak flushes, since the cake it came from flushed all small mushrooms (roughly 1"), or will I be OK, seeing as the liquid culture colonized quickly and just fine?

I have grains prepared and would hate to start a whole new liquid culture and wait, but of course I will if I must...


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InvisibleEvilMushroom666
Heretic
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Registered: 11/18/09
Posts: 10,289
Loc: Canada Flag
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Re: Weak genetics? [Re: thomas_knobs]
    #14047631 - 03/01/11 09:48 AM (12 years, 10 months ago)

When using a sugar based LC (Which I assume you are) you should always
shoot a single test jar before moving onto bigger projects. Just because
a LC looks like it has grown out fast and is ready to go, you can never
know 100% just by looking at it.

Better to have 1 BRF cake or jar of rye/wbs contaminate on you then
your entire project.


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Offlinebbt0009
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Re: Weak genetics? [Re: EvilMushroom666]
    #14047640 - 03/01/11 09:52 AM (12 years, 10 months ago)

Yea man i've done four LC's recently with no success. They all contamed


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It was all a dream...


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OfflineHakim0777
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Re: Weak genetics? [Re: thomas_knobs]
    #14047681 - 03/01/11 10:04 AM (12 years, 10 months ago)

Quote:

thomas_knobs said:
I recently made an LC by stabbing a small mushroom stem (the only viable remaining living specimen that I had at the time). The culture has grown nicely and is ready to use for grain inoculation, but i'm a little concerned as to the genetics. Should I be worried about producing a strain with weak flushes, since the cake it came from flushed all small mushrooms (roughly 1"), or will I be OK, seeing as the liquid culture colonized quickly and just fine?

I have grains prepared and would hate to start a whole new liquid culture and wait, but of course I will if I must...




Youre gonna get a mixed bag of potency with MS inoculation.


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Offlinethomas_knobs
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Registered: 09/23/10
Posts: 330
Last seen: 1 year, 10 months
Re: Weak genetics? [Re: Hakim0777]
    #14048707 - 03/01/11 02:05 PM (12 years, 10 months ago)

I am going to test the LC for contams before moving on...but my question has to deal with whether or not it would be wise to continue through with this considering the issue of cloning from a small mushroom. It was not just a pin, its cap had opened entirely and it was about 1", which probably had to do with environmental factors (FAE). I don't want to have 10 tubs of tiny mushrooms, in other words...


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InvisibleEvilMushroom666
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Re: Weak genetics? [Re: thomas_knobs]
    #14048716 - 03/01/11 02:06 PM (12 years, 10 months ago)

Do a small scale fruiting to see if the culture has the required characteristics
that you are looking for. If not start again from MS, or jump into agar and
you will be isolating your own strains in no time.


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Offlinethomas_knobs
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Registered: 09/23/10
Posts: 330
Last seen: 1 year, 10 months
Re: Weak genetics? [Re: EvilMushroom666]
    #14052267 - 03/02/11 12:03 AM (12 years, 10 months ago)

hmm...suppose i'll just give it a go, i have a LC that's all ready from spore solution, should I just use that instead? The myc from this one looks healthier and grew faster though, despite it being from a puny mushroom. Is it still possible that I get healthy plump fruits in theory though, or are they now genetically disposed to being like the lame parent shroom?


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InvisibleEvilMushroom666
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Re: Weak genetics? [Re: thomas_knobs]
    #14052299 - 03/02/11 12:14 AM (12 years, 10 months ago)

The tiny shrooms could have been a product of their environment, or
even bacteria. Shoot a test jar of your LC first and see if its clean.

If it is, make a few jars and see what happens :shrug:


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