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Offlinebosebreez
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Registered: 02/17/11
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HELP..already inoculated.
    #14044535 - 02/28/11 07:15 PM (12 years, 10 months ago)

hey, ok i bought myco bags of the actual myco bag site. and i got treasure coast spores off hawks eye. i choose that strain because it is the only ones I've tried and a friend grew them already. so i wanted to try. i injected the bag at first with half the syringe. i have it in a tub with 2 heating pads (ghetto i know) but it IS holding temperature. 84-90 degrees. i mushed it up before injecting and for a week saw no growth. so i used the rest of the syringe.i see it is creating humidity in the bag.. ....do i still have a chance for success?...even using the whole syringe? is there still a chance i will get mycelium growth? i jus re-injected the bag with the rest of the syringe earlier today...

i AM a NOOB i know..noted.


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Offline3n1gm4
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Re: HELP..already inoculated. [Re: bosebreez]
    #14044625 - 02/28/11 07:27 PM (12 years, 10 months ago)

84-90F is too hot IMO, why not just room temp, 65-70F?


--------------------
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Offlinebosebreez
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Re: HELP..already inoculated. [Re: bosebreez]
    #14044631 - 02/28/11 07:28 PM (12 years, 10 months ago)

man yall reading my post but not replying. hah. i jus really want this to succeed.. i could post pictures if needed of how it looks. ghetto style incubator. but its holding temp. so. yea.


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OfflinePsilocyentist
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Re: HELP..already inoculated. [Re: bosebreez]
    #14044647 - 02/28/11 07:30 PM (12 years, 10 months ago)

Its holding the wrong temp. 84-90 your bags are already fucked with contams I'm guessing. Get it off of the fucking heating pads as RR says "heating pads have no place in mycology." Colonize at room temp and see if anything starts growing.


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Offline3n1gm4
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Re: HELP..already inoculated. [Re: bosebreez]
    #14044654 - 02/28/11 07:31 PM (12 years, 10 months ago)

84-90F will succeed in growing a contamination really well...UTSE(use the search engine)


--------------------
http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!


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Offlinebosebreez
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Re: HELP..already inoculated. [Re: 3n1gm4]
    #14044657 - 02/28/11 07:31 PM (12 years, 10 months ago)

well before i started i did ALOT of reading. and everything i read said that it should be around 85 degrees after inoculation for mycelium to grow, and after colonization. to lower temp to about 77 n with a light to fruit... but im still waiting to see mycelium


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OfflinePsilocyentist
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Re: HELP..already inoculated. [Re: bosebreez]
    #14044666 - 02/28/11 07:32 PM (12 years, 10 months ago)

Quote:

bosebreez said:
well before i started i did ALOT of reading. and everything i read said that it should be around 85 degrees after inoculation for mycelium to grow, and after colonization. to lower temp to about 77 n with a light to fruit... but im still waiting to see mycelium



What you read was outdated. Look around here for more accurate info. Room temp colonization is not only fine, its optimal (75-78).


--------------------
I know, I know you probably scream and cry

That your little world won't let you go



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Offline3n1gm4
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Re: HELP..already inoculated. [Re: bosebreez]
    #14044670 - 02/28/11 07:33 PM (12 years, 10 months ago)

Quote:

bosebreez said:
well before i started i did ALOT of reading. and everything i read said that it should be around 85 degrees after inoculation for mycelium to grow, and after colonization. to lower temp to about 77 n with a light to fruit... but im still waiting to see mycelium



read the dates on posts and go to this site and watch every video clip before you try again
http://www.mushroomvideos.com/


--------------------
http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!


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Offlinebosebreez
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Re: HELP..already inoculated. [Re: 3n1gm4]
    #14044719 - 02/28/11 07:38 PM (12 years, 10 months ago)

damn go figure i READ alot before i did it too. i checked alot and around 85 was what i kept getting. thank you though. appreciate the help. HOPEFULLY!! maybe...its not contaminated, n i still have a chance HAh...but WILL do next time...


ALSO i have another growbag unused....i dont know how to store it. it came free with my other one. i put it in the fridge. i dont know if this is ok or not...but either way.. i WILL try again if this is unsuccessful
....
my grow box is ghetto though...you seasoned cultivators...would for sure get a laugh. .....WELL we are all noobs at one point in time.


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Offline3n1gm4
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Re: HELP..already inoculated. [Re: bosebreez]
    #14044737 - 02/28/11 07:40 PM (12 years, 10 months ago)

Go watch the videos on that site I linked and read there, it is all correct info there.


--------------------
http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!


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Offlinebosebreez
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Posts: 80
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Re: HELP..already inoculated. [Re: 3n1gm4]
    #14044775 - 02/28/11 07:45 PM (12 years, 10 months ago)

Thank you very much.


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Offlinebosebreez
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Re: HELP..already inoculated. [Re: bosebreez]
    #14045135 - 02/28/11 08:44 PM (12 years, 10 months ago)

i REALLY hopefull that my bag is not contaminated. i lowered the temp...so we'll see.


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InvisibleDZ
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Registered: 01/25/09
Posts: 381
Re: HELP..already inoculated. [Re: bosebreez]
    #14045195 - 02/28/11 08:52 PM (12 years, 10 months ago)

I didn't read all the posts so ignore this if it's been noted. Using half of a spore syringe was such a waste, let alone an entire syringe. And room temp is what you want for incubation temps, if you're comfy they're comfy.

Good luck


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Offlinebosebreez
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Re: HELP..already inoculated. [Re: DZ]
    #14045374 - 02/28/11 09:18 PM (12 years, 10 months ago)

well like said i AM a noob. and i DID read up on it not like i DIDnt research. however alot of what i DID read was for useing cakes and PF tek or however it is referred. and im not sure how the process would differ using bags so alot of what i looked for was bag specific and as pointed out by another member was evidently i was reading OLD posts. i got excited and admitt i jumped into it a bit to premature. but i did flip through those videos(other reply). and i like the idea of using a bag a little more though becuase it IS less of a process. and less equipment. so i know that i want to use the bags. trial and error i suppose. but i dont know if my bag is contaminated...there is not growth and no sign of contam. (looked up pictures)...i suppose once it does show growth i will know. like said i researched shit i read from diff sites. copy and pasted. took notes.. in notepad and everything. Actually im going to paste that file, i dont know so yall can see what i went off of. or wutever. for yall who actually feel like reading and helping me out with anything you have to say.


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Offline3n1gm4
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Re: HELP..already inoculated. [Re: bosebreez]
    #14045448 - 02/28/11 09:27 PM (12 years, 10 months ago)

Its a crap shoot, you may have a contam, you may get lucky and have a successful grow. Waiting is 90% of this hobby, I would suggest getting another syringe if you can and following a proven tek that is up to date while you are waiting on your other bag, so if your first bag does contam you still have something going and you don't have to wait as long for the next one to start. You could even do a race on the bags and see if the new one passes the 1st one. Just a suggestion...:shrug:


--------------------
http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!


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Offlinebosebreez
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some of the notes and pasted posts [Re: bosebreez]
    #14045454 - 02/28/11 09:27 PM (12 years, 10 months ago)

here is one of the files i had with notes, pasted things i read off posts, and actuall bag instructions off the site i got it from. and the 85 degree incubate said on multiple posts, and sites so thats y i had went with 'about' 85 degrees.


tub
small heater

incubate @ 85  degrees
fruiting.. 73-76 degrees

the shrooms will know to friut as the temperture drops

small benable lamp

which kind of light doenst matter, it jus tells the shrooms when to friut n which direction to grow

humidifier



Upon arrival unfold the Bag, allowing the extra material to stand straight up.

Locate the small foam disk sealed and attached in a plastic housing near the base of the MyShroominBag™. This disc is the self-healing injector site. Note: The disc will be covered by a small plastic shield. Do not attempt to remove the plastic covering the disc, you will inject through it. Sterilize a small section of the outside of the MyShroominBag™ (on the plastic shield) directly over the foam disc with an alcohol soaked wipe. With the same alcohol soaked wipe, sterilize the needle on your culture syringe.

Inoculate the substrate (material inside the bag) with culture syringe solution by inserting the needle through the plastic shield and foam disc at the previously sterilized spot. Squirt 3cc - 10cc of culture solution directly into the substrate material.

DO NOT insert the needle through the white filter patch found near the top of the Bag.

Place the MyShroominBag™ in a dark location and incubate at species specific parameters. It is recommend to store the Bag fully extended as developing Mycelia will give off gases that will slowly expand the interior of the Bag. This ballooning effect is crucial for proper air exchange and culture development.



Note: During drier summer months it is advisable to store the Bag in a humid location. Excessively dry environments may draw moisture from the Bag.

Because colonization will begin in the interior of the substrate material it may take between 14-20 days for you to notice the spreading of colonizing mycelium towards the outside of the Bag. Speed of colonization is directly affected by incubation temperatures, please check to make sure you are incubating the bag at the correct temperature for the species you are attempting to cultivate. Typical colonization time is between 21-30 days for most species and strains when incubated properly.

When colonization is 90% or 100% complete, move MyShroominBag™ to a location with indirect light (indoor light or filtered sunlight is best). Allow the Bags to receive 12 hours of indirect light per day (direct fluorescent lighting works as well).



GENTLY massage bag at 30 % colonization

rubbing alcohol

cotton balls



tub in tub method. bag in small tub in side another tub
heating pad.
water...fish tank heater.?

perlite ..water....for humidity



1. Unfold the bag (So the filter patch can do its thing)

2. Inject the substrate (Inject between the plastic wall of the bag... this allows spores to cover a lot more surface area than if you just inject the middle of the substrate. DO NOT INJECT THE MIDDLE OF THE SUBSTRATE or your bag may not grow anything)

3. Mash the bag (Yes, mash it... MycoBags feel like bricks when you get 'em... after inoculating, you must mash the substrate inside the bag... mash it good... once it feels like potting soil, you're all set.)

4. Wait 3-14 days until you see growth (Usually 4-6 days)

5. Once you see growth, MASH the bag again. (Mash the mycelia, myc will disappear when you mash it, this is GOOD, it will spread like WILDFIRE when it grows back... greatly speeding up colonization time)

6. After the bag recovers from the LAST mash (usually 2-3 days), MASH again.

7. Let the bag finish colonizing. If you mashed correctly, full colonization should only be 2-3 days away.

8. Once colonized, allow the bag to get light (MycoBags are cool and you can let 'em colonize in the light if you wish... but if you expose the bag to light too early, the bag may pin before it finishes colonizing... I NEVER HAVE A PROBLEM WITH THIS... and I colonize bags in the light.... bags are fun to watch for n00b mycologists... why not watch it grow?)

9. Pins should come 2 days- 2 weeks after full colonization... and shrooms should follow. Shrooms take 4-10 days to grow from pin to mushroom.

10. Cut open the bag and harvest your shrooms... spray the inside of the bag with a little water (Keep the patch dry) and re-seal the bag.

11. Repeat steps 9 and 10 until bag is done fruiting (Most cakes contaminate when they're done producing... don't feel bad if your bag contams after 3-4 flushes.)


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Invisiblebiologys
Mycologist in Trainning
Male


Folding@home Statistics
Registered: 12/21/09
Posts: 4,622
Re: some of the notes and pasted posts [Re: bosebreez]
    #14045474 - 02/28/11 09:31 PM (12 years, 10 months ago)

alot of outdated info on there...as stated before do some reading around

do searches and check for newer posts, no more then 1-2 years old...also on the search page it gives the option to only view posts by trusted cultivators...they've proven themself for knowing what to do..


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Offlinebosebreez
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Female

Registered: 02/17/11
Posts: 80
Last seen: 12 years, 8 months
Re: HELP..already inoculated. [Re: 3n1gm4]
    #14045485 - 02/28/11 09:32 PM (12 years, 10 months ago)

haha yea i plan on ordering another syringe. thing is i dont know if the extra bag i got (unused ovbiously) will still be ok to use when i get the new syringe. i got the bags like ..ill go ..a week and a half ago, only used one.


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Invisiblebiologys
Mycologist in Trainning
Male


Folding@home Statistics
Registered: 12/21/09
Posts: 4,622
Re: HELP..already inoculated. [Re: bosebreez]
    #14045491 - 02/28/11 09:33 PM (12 years, 10 months ago)

the bag should be fine as long as prepped properly..i've had bags/jars sit for over a month and still inoculate and colonize fine.


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Offlinebosebreez
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Female

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Re: some of the notes and pasted posts [Re: biologys]
    #14045499 - 02/28/11 09:34 PM (12 years, 10 months ago)

thank you. im going to do things different next time around hah.


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