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OfflineFunkjunkie
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64F - 67F too cold for Germination??
    #14040310 - 02/28/11 03:51 AM (12 years, 10 months ago)

I have 1 grain jar thats 5 days old and one jar thats 3 days old, no signs of growth. Both were MS inoculated .  The MS syringe i used for the 5 day old jar was really diluted, maybe 3 single visible specs of spores in the syringe. So i used another syringe that had more spores in it for the 3 day old jar

I know patience is a virtue in this hobby, but is 64 - 67 too cold for spore germination??..Im about to move the jars to the room where my boiler and water heater are, much warmer in there.


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Invisiblebiologys
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Re: 64F - 67F too cold for Germination?? [Re: Funkjunkie]
    #14040318 - 02/28/11 03:55 AM (12 years, 10 months ago)

they'll germinate fine...a tad slower then normal but they'll do fine.


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OfflineKingMob
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Re: 64F - 67F too cold for Germination?? [Re: biologys]
    #14040373 - 02/28/11 04:46 AM (12 years, 10 months ago)

Any part of the process is best to do in the 70's.

So if you can move the jars to a warmer room where you can be sure the temps will remain in the 70's (no higher than 80 degrees tho) it will speed up colonization.

But as biologys said, you can leave them in their current temps without any harm, if it is more convenient for you.


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OfflineFunkjunkie
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Re: 64F - 67F too cold for Germination?? [Re: KingMob]
    #14040388 - 02/28/11 05:01 AM (12 years, 10 months ago)

thanks guys


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Offlinechains


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Re: 64F - 67F too cold for Germination?? [Re: Funkjunkie]
    #14040458 - 02/28/11 06:03 AM (12 years, 10 months ago)

I 'incubate' mine in the high 60's and it works fine, just might be abit slower than the rest but its more convenient for me and might even help for a lower contam rate.


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Offlinehiimuseless
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Re: 64F - 67F too cold for Germination?? [Re: Funkjunkie]
    #14040613 - 02/28/11 07:43 AM (12 years, 10 months ago)

Mine do fine in 65 - 70 range. It would take a lot less time to show growth if you started with a liquid culture or agar instead of multispore...


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InvisibleDoc_T
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Re: 64F - 67F too cold for Germination?? [Re: hiimuseless]
    #14040629 - 02/28/11 07:48 AM (12 years, 10 months ago)

5 days is too soon to worry.

But yeah, 70+ is better for germination. Once you get mycelium going, 65-70 is just fine for jars.
I use an incubation chamber, an unpowered incubator. I put jars in a styro cooler with a jar of warm water.
Keep a thermometer in there, and put the water jar in the microwave once a day or as needed.
You can easily keep you jars at a comfy 75. Don't go over 80.


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OfflineRogerRabbitM
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Re: 64F - 67F too cold for Germination?? [Re: chains]
    #14040634 - 02/28/11 07:50 AM (12 years, 10 months ago)

The boiler room is probably better as long as it doesn't get over 80F.  It will work at your temps, but slower.
RR


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Offlinemister brown 420
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Re: 64F - 67F too cold for Germination?? [Re: Doc_T]
    #15874494 - 02/28/12 02:12 AM (11 years, 10 months ago)

Quote:

Doc_T said:
5 days is too soon to worry.

But yeah, 70+ is better for germination. Once you get mycelium going, 65-70 is just fine for jars.
I use an incubation chamber, an unpowered incubator. I put jars in a styro cooler with a jar of warm water.
Keep a thermometer in there, and put the water jar in the microwave once a day or as needed.
You can easily keep you jars at a comfy 75. Don't go over 80.




Way to keep it low fy.. verry cool! Doc T with the info! FTW

I might have to try this on my next grow


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OfflineJohnnyZebra
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Re: 64F - 67F too cold for Germination?? [Re: mister brown 420]
    #15881539 - 02/29/12 02:45 PM (11 years, 10 months ago)

LOL.. i had a similar concern. I inoculated my 1/2pint jars 5 days ago and they have been incubating in an aluminum storage unit with swinging doors and shelves, roughly 65 degrees in there. When i say roughly it varies from 63-70 max. I started to worry seeing no growth whatsoever. Listen to people on this forum because IT IS all about patience. no way i could blast my heat and keep it at 80, too expensive. and im not willing to risk any fire hazards or contamination rigging up some source of heat in there. Just today, literately about 1 hour ago, i noticed the first spot of healthy mycellium. only about 1-2cm in diameter but its there and its very noticeable. Bright white fuzzy looking..Give it some more time. AWESOME to finally see some results after all those sterilization precautions and practices i took. Let it ride out a few more days, those spores will make it!


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OfflineTheCannabisSativa
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Re: 64F - 67F too cold for Germination?? [Re: Doc_T]
    #17158387 - 11/04/12 09:36 AM (11 years, 2 months ago)

Quote:

Doc_T said:
5 days is too soon to worry.

But yeah, 70+ is better for germination. Once you get mycelium going, 65-70 is just fine for jars.
I use an incubation chamber, an unpowered incubator. I put jars in a styro cooler with a jar of warm water.
Keep a thermometer in there, and put the water jar in the microwave once a day or as needed.
You can easily keep you jars at a comfy 75. Don't go over 80.





sorry for the old thread bump but I like this idea, just want to make sure the jars could breathe threw the styro cooler fine? ... is that why you don't use a regular plastic cooler?


I can't believe the problem I've been having getting jars to germinate, it worked at my lower then 70 degree temps fine in the past. I went threw 4 syringes already, the jars from 2 of them have been started over after doing nothing for several weeks. It's only been a week with the new ones but no signs yet and after searching a little on here I'm thinking it must be the temps. I thought it was cause the air was to dry so I even had some jars in the SGFC (not being used for cakes at the time)) but that didn't help. 2 different syringes from 2 trusted sponsors, not 1 sign on contam either just no growth. temps just a little over 65' steady - that must be the problem.

I put a few jars in the boiler room and a few on top of the freezer so it warms the bottom to see if that helps. I'm going to grab a styrofoam cooler and try your idea out as well.


Edited by TheCannabisSativa (11/04/12 09:41 AM)


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Offlinetwistedty
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Re: 64F - 67F too cold for Germination?? [Re: TheCannabisSativa]
    #17158389 - 11/04/12 09:37 AM (11 years, 2 months ago)

no, blah this thread is old as tits, just make a new one


Edited by twistedty (11/04/12 09:37 AM)


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