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OfflineBurblesV
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Re: Micro propagation at home. [Re: Doc_T]
    #14039345 - 02/27/11 10:32 PM (13 years, 5 days ago)

Ya'll have seen mushmush/Una's website right?

If not, your missing out.

http://www.mushmush.nl/?page=begin\gallery\tissue_culture

click through that inspirational stuff sometime..

I have been trying to figure out his "secret cactus culture' stuff and I am pretty sure I almost got it down - trick is coconut milk and a certain hormone or two.. .can't remember but I posted all the articles I found on cacti micropropagation on my site.

But this is the sort of stuff you see on his site:




I am mostly interested in the callus tissue culture -- imagine giving it just the right conditions to produce mescaline, grow faster, and just be an unidentifiable green blob :laugh:

Edited by Burbles (02/27/11 10:36 PM)

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Offlinebrshroomer
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Re: Micro propagation at home. [Re: Burbles]
    #14039990 - 02/28/11 12:32 AM (13 years, 5 days ago)

not just that, if it proves true that the plants need no light during micropropagation then this is some seriously important stuff.

you could send clones MUCH easier in the mail, i can think of a lot of plants that i would like to have but can't because they wouldn't withstand the trip(salvia for one)

even for cannabis as the OP is working with there is some utility, much better to send a green tiny blob in the mail than seeds. much lesser risk of crushing too.


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Invisiblecacharstar
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Re: Micro propagation at home. [Re: Burbles]
    #14040075 - 02/28/11 12:54 AM (13 years, 5 days ago)

Thread:



Small world.  My copy of Plants from test tubes arrived about a week ago. I have a month or so fantasizing about the uses of a kitchen tek for cannabis. So who wants to help write one? I found this research done on the best adjustment of the ms medium for hemp but am having trouble understanding exactly how to carry out the mixing without a firm background in chem.. Perhaps we can help each other.:rasta: Here is the linkhttp://www.pakbs.org/pjbot/PDFs/41%282%29/PJB41%282%29603.pdf
I want to revive some strains. Imagine the possibilities.

Op... Keep us updated.


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Invisiblecacharstar
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Re: Micro propagation at home. [Re: brshroomer]
    #14040100 - 02/28/11 01:01 AM (13 years, 5 days ago)

Quote:

brshroomer said:
not just that, if it proves true that the plants need no light during micropropagation then this is some seriously important stuff.

you could send clones MUCH easier in the mail, i can think of a lot of plants that i would like to have but can't because they wouldn't withstand the trip(salvia for one)

even for cannabis as the OP is working with there is some utility, much better to send a green tiny blob in the mail than seeds. much lesser risk of crushing too.





I think light is needed and depending on the developmental stage of the explant, the light could have disastrous effects. for instance if one were so inclined to send clones via mail in a ms medium, I assume the light cycle would need to be achieved with a 12 volt battery and a led; However I have no experience on how light effects plants in vitro...


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InvisibleDoc_T
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Re: Micro propagation at home. [Re: cacharstar]
    #14040530 - 02/28/11 07:01 AM (13 years, 5 days ago)

I got a PM asking for updates, but not much to say yet.
I checked my seeds last night. One had a root about an inch long, but hadn't gotten out of the seed hull.
When I picked it off, the seedling was discolored and looked icky. :frown:
Root seemed healthy, I put a few bits into a dish.
And a few bits of the leaf end went into another dish, just to see.

So I guess I still need a plant, I'm stuck at Step 0.


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OfflineDjcorbetto
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Re: Micro propagation at home. [Re: Doc_T]
    #14040651 - 02/28/11 08:04 AM (13 years, 5 days ago)

I cant remember what plant you are trying to propogate, was it cannabis im a bit dopey at the moment and got a rubbish internet signal so cant go back. if it is cannabis do you not have any friends who live near you who grow or know someone who grows, and get a cutting from them. if you explain to them what you are doing then they might be more inclined to help you out and moeny always helps out. keep some of the plant to grow for a cutting so you have a reasonably steady source of plant material.

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InvisibleDoc_T
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Re: Micro propagation at home. [Re: Djcorbetto]
    #14040658 - 02/28/11 08:07 AM (13 years, 5 days ago)

Cannabis, yes. Yeah, I might be able to get a cutting from a friend of a friend. I can trade for it. :smile:

Other than salvia, what plants would home hobbyists want to grow this way?
Orchids, I know. What else? Cactus, maybe?


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Offlinecaricapapaya
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Re: Micro propagation at home. [Re: Doc_T]
    #14040947 - 02/28/11 09:56 AM (13 years, 5 days ago)

Doc_T

did you sterilize the seeds?

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InvisibleDoc_T
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Re: Micro propagation at home. [Re: caricapapaya]
    #14040960 - 02/28/11 09:59 AM (13 years, 5 days ago)

Dipped in dilute bleach, like I usually do when germinating seeds. Then between wet paper towels in the dark.

I figure the leaves can be washed with sterile water, h202, whatever before rendering them for donor plugs.


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Offlinecaricapapaya
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Re: Micro propagation at home. [Re: Doc_T]
    #14041044 - 02/28/11 10:26 AM (13 years, 5 days ago)

I thought you were germinating in vitro.

sterilizing tender new leaves can be problematic. not saying it cant be done, but its easier to sterilize seeds.

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InvisibleDoc_T
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Re: Micro propagation at home. [Re: caricapapaya]
    #14041149 - 02/28/11 10:48 AM (13 years, 5 days ago)

I could germinate on agar, I've got extra dishes on hand.

I think a new shoot is going to be basically sterile anyway, I don't see why there would be any contamination?


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OfflineHarveyWalbanger
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Re: Micro propagation at home. [Re: Doc_T]
    #14041159 - 02/28/11 10:49 AM (13 years, 5 days ago)

.

Edited by HarveyWalbanger (03/07/11 02:50 PM)

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OfflineBurblesV
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Re: Micro propagation at home. [Re: HarveyWalbanger]
    #14043596 - 02/28/11 05:16 PM (13 years, 4 days ago)




http://www.lycaeum.org/mv/anagrams/cannabis_tissue_culture.html


Cannabis tissue culture :laugh:


Why would the plant need light to grow if it is using the sucrose in the mixture? the callus tissue culture doesn't do a significant amount of photosynthesis, and light is primarily only used to aid in proper formation of the small plantelts... I.e grow up and not etioliate..

No need for light during the callus tissue formation AFAIK... and that would be the way you would want to send strains through the mail anyways for obvious reasons (decreased to no THC levels, unidentifiable, probably more hardy)

Edited by Burbles (02/28/11 05:22 PM)

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OfflineMicroppose
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Re: Micro propagation at home. [Re: Burbles]
    #14043745 - 02/28/11 05:34 PM (13 years, 4 days ago)

Excellent post Burbles.
So this specimen in figure four, is after six weeks of growth on MS in a slant?

Edited by Microppose (02/28/11 05:35 PM)

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OfflineBurblesV
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Re: Micro propagation at home. [Re: Burbles]
    #14043938 - 02/28/11 05:55 PM (13 years, 4 days ago)

Not sure how old the 4th picture is, the third  is 6 weeks, and I think the first two are only 3 weeks. The 4th picture is probably like 7-8 weeks, which seems kind of like a long time, but given this can be an exact clone of the plant you like, and you can do hundreds or thousands of them.. that isn't bad at all.

Also you can skip step 1-3. You really only need a small sterile shoot, which than it can be rooted.. this doesn't allow you to produce as many for obvious reasons - but as long as the cutting has a small place to grow new leaves/shoots - you could essentially use any piece of the plant.
That would easily result in hundreds from a good plant.
I haven't done much with micro-prop. Its my summer project though!

I will also be getting a ton of supplies, like ideal tissue culture jars (about 4 oz polypropylene, with a cap that functions like a petri dish cap... allowing for air flow but prevents contams.

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Offlinethe man
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Re: Micro propagation at home. [Re: Burbles]
    #14052564 - 03/02/11 01:45 AM (13 years, 3 days ago)

well you need more then rooting and nutrients. unless you are just rooting cuttings essentially.  need to get a calus going of sort of stem cells take that get it to root and then to grow stem all using dif hormones which i dont think are easy to get ahold of as i tried a long time ago.  if there is a way let me know would be cool for orchids and stuff. just getting those dang hormones.  keep us updated if you find out if you can find them. be awesome send a callus culture of MJ much easier then a cutting    send a og kush wedge :smile:

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OfflineBurblesV
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Re: Micro propagation at home. [Re: the man]
    #14052972 - 03/02/11 06:26 AM (13 years, 3 days ago)

I bought 25 grams of NAA and 5 grams of 6-BAP for about 10 bucks each I believe, many others are for sale as well... all over the place..

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InvisibleDoc_T
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Re: Micropropagation at home. [Re: Doc_T]
    #14055482 - 03/02/11 04:05 PM (13 years, 3 days ago)

Well fuck me running- this might work!

Hard to see in the pic, but I think I have some shoots growing out of root fragments.
Largest is 3-4mm, better pics should happen in a couple days.



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OfflineMicroppose
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Re: Micropropagation at home. [Re: Doc_T]
    #14055595 - 03/02/11 04:30 PM (13 years, 2 days ago)

Shweet mang, what was your final medium mixture?

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OfflineBurblesV
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Re: Micropropagation at home. [Re: Microppose]
    #14055949 - 03/02/11 05:31 PM (13 years, 2 days ago)

I would be shocked if growth were occurring that fast!

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