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Djcorbetto
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Registered: 01/12/11
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Re: Micro propagation at home. [Re: Burbles]
#14017809 - 02/24/11 01:20 AM (13 years, 9 days ago) |
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I may have written it down wrong, infact im sure i remember seeing different hormones because it provoked me to look all over google and ebay to see how much they would cost me to purchase. I will look again and see if i can find the link for the page
EDIT: Found it
The media is M&S, with 15 g/L of sucrose, pH 5.8 before sterilizing (balanced with KOH) and 2mg/L kinetin, 0,2 mg/L IAA and 10 g/L agar.
it doesnt specify whether its for roots or shoots but then im still looking at his follow up as he got shoots on the plant material but so fare dont see a bit on roots.
Link: The media is M&S, with 15 g/L of sucrose, pH 5.8 before sterilizing (balanced with KOH) and 2mg/L kinetin, 0,2 mg/L IAA and 10 g/L agar.
i saw on that page there was a mention of a salvia recipe but they only put down what they had researched: "...Then placed in sterile glass culture tubes containing Murashige and Skoog medium supplemented with 2% glucose, Gamborg’s Vitamin solution, 0.2% Phytagel, and 5 μM 6-benzylaminopurine
Edited by Djcorbetto (02/24/11 01:30 AM)
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Djcorbetto
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Re: Micro propagation at home. [Re: Djcorbetto]
#14017892 - 02/24/11 01:48 AM (13 years, 9 days ago) |
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Oh and theres this:http://www.lycaeum.org/mv/anagrams/cannabis_tissue_culture.html
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Burbles
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Re: Micro propagation at home. [Re: Djcorbetto]
#14020323 - 02/24/11 02:33 PM (13 years, 9 days ago) |
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Awesome, thanks for that link!
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Djcorbetto
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Re: Micro propagation at home. [Re: Burbles]
#14020503 - 02/24/11 03:06 PM (13 years, 9 days ago) |
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No problem, hopefully it helps you so you will make up a method of doing this which in turn will help me
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Djcorbetto
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Re: Micro propagation at home. [Re: Djcorbetto]
#14020951 - 02/24/11 04:44 PM (13 years, 8 days ago) |
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Doc_T
Random Dude




Registered: 03/06/09
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Loc: Colorado
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Re: Micropropagation at home. [Re: Doc_T]
#14034742 - 02/27/11 08:03 AM (13 years, 6 days ago) |
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Quick question about process: I didn't have a plant handy so I'm sprouting some seeds. I guess I need chlorophyll for this to work, not just a root tip? But even a cotyledon should work, no need for a 'real leaf'?
Also, I don't think I can punch discs out of a leaf with a needle, I'm just going to cut tiny flakes with scissors. I hope that's not cheating.
-------------------- You make it all possible. Doesn't it feel good?
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doses
Obsessive Purist



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Re: Micro propagation at home. [Re: Doc_T]
#14034828 - 02/27/11 08:51 AM (13 years, 6 days ago) |
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Micro propagation at home. [Re: doses]
#14034851 - 02/27/11 08:58 AM (13 years, 6 days ago) |
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Quote:
doses said: http://www.mediafire.com/?uy2jiizx2hz
Quote:
Moving the plantlets to regular soil The key to sucessfully transferring your in-vitro plantlets into soil is to be very fastidious about washing off all the TC media from the roots.
Words of wisdom right there. Thanks!!!
-------------------- You make it all possible. Doesn't it feel good?
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Djcorbetto
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Re: Micro propagation at home. [Re: Doc_T]
#14035704 - 02/27/11 12:24 PM (13 years, 6 days ago) |
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to cut the leaf up you could use a scalpel instead scissors
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Burbles
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Re: Micro propagation at home. [Re: Djcorbetto]
#14036320 - 02/27/11 02:29 PM (13 years, 6 days ago) |
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Plants from a test tube... Its one of the best books out there...
Edit: Damn it, shroomery won't let me upload djvu files...
Here... http://www.thelaboratory.org/talk/index.php?topic=18.msg1285#msg1285
Edited by Burbles (02/27/11 02:31 PM)
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Burbles
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Re: Micro propagation at home. [Re: Burbles]
#14036340 - 02/27/11 02:33 PM (13 years, 6 days ago) |
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Also you shouldn't need chlorophil at all.. if your medium has sucrose...the tissue callus does not do photosynthesis to any significant extent and could live in the dark if need be...AFAIK
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HarveyWalbanger
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Re: Micro propagation at home. [Re: Doc_T]
#14036356 - 02/27/11 02:35 PM (13 years, 6 days ago) |
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Quote:
Doc_T said: That's a good one?!?!
Edit- apparently nonmagical but a fun cactus anyway. I may get it if it's there next week, but not worth driving back for.
Supposedly it's active, but it's not mesc.
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jokefox
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Registered: 12/22/09
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hmm, lets see where this goes
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Microppose
Things Maker



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Re: Micro propagation at home. [Re: jokefox]
#14036632 - 02/27/11 03:43 PM (13 years, 6 days ago) |
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I have no idea what's going on here... So you are essentially trying to grow a plant, but only feeding off of the agar like a fungus? With the assistance of the plant food of course. I am curious to see your experiment summary. GL
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jokefox
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Re: Micro propagation at home. [Re: Microppose]
#14036655 - 02/27/11 03:47 PM (13 years, 6 days ago) |
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Quote:
Azurascender said: I have no idea what's going on here... So you are essentially trying to grow a plant, but only feeding off of the agar like a fungus? With the assistance of the plant food of course. I am curious to see your experiment summary. GL
go type in micro propagation to google, and you will be amazed
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Microppose
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Re: Micro propagation at home. [Re: jokefox]
#14036785 - 02/27/11 04:10 PM (13 years, 6 days ago) |
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OMG, rather interesting. What kind of plant has he chosen? From what I understand, you get plant material and cell multiplication, but not the features of the plant?
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jokefox
Top of the chain



Registered: 12/22/09
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Re: Micro propagation at home. [Re: Microppose]
#14036829 - 02/27/11 04:19 PM (13 years, 6 days ago) |
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basically you grow a plant from a tiny piece called a platlette or somthing , that calluses, the shoots & roots , then you have a clone plant of the original
theory is a thousand plants from a leaf
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Burbles
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Re: Micro propagation at home. [Re: jokefox]
#14036897 - 02/27/11 04:30 PM (13 years, 5 days ago) |
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Or you could just grow the cells for alkaloid production, perhaps... I.e grow root cells of sassafrass in a large liquid culture - its a bit different than true micropropagation, but the skills are the same.
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brshroomer
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Re: Micro propagation at home. [Re: Burbles]
#14037298 - 02/27/11 05:49 PM (13 years, 5 days ago) |
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Quote:
Burbles said: Also you shouldn't need chlorophil at all.. if your medium has sucrose...the tissue callus does not do photosynthesis to any significant extent and could live in the dark if need be...AFAIK
this is ...awesome.
this could be a solution to mailing plants.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Micro propagation at home. [Re: Microppose]
#14038105 - 02/27/11 07:57 PM (13 years, 5 days ago) |
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Quote:
Azurascender said: OMG, rather interesting. What kind of plant has he chosen?
The kind I had a few seeds of laying around close at hand. 
Yeah, theory is agar with plant food and 'stuff', grow clone plants from tiny shreds.
We'll see what we see.
-------------------- You make it all possible. Doesn't it feel good?
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