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Wise Toad


Registered: 06/08/10
Posts: 2,690
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Healthy mycelium?
#14036184 - 02/27/11 01:59 PM (12 years, 10 months ago) |
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I want to make sure these jars are all good to go and not contams, its taken them about a month from MS to fully colonize; half the month was spent in the low to mid 60s, the other half was in the 70s.      
These are from rye jars that has been consolidating for about a week and were shaken today to prepare for GLC 
I did a GLC on thursday which resulted in   and the final jar . A mistake was made however as the 60cc syringe of myc/water was injected into a jar 800 ml full of water(instead of 550ml); so it was heavily diluted. 3ccs of that was injected into a grain test jar immediately after making the GLC, the test jar has yet to show any growth after two days. The grain jar used for the GLC had only 3 days to consolidate. On tuesday 7 pint grain jars were sterilized to test out GLCs on as they become available, how long will they last without(contaminating/drying out)? The two 60cc syringes sterilized via Shea25s syringe sterilization tek were still a little bit warm when they were injected which worries me now.
Im probably just being paranoid as usual but would like some feedback
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Humility
Working on it



Registered: 10/07/08
Posts: 6,745
Last seen: 6 years, 11 months
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Re: Healthy mycelium? [Re: Wise Toad]
#14037493 - 02/27/11 06:19 PM (12 years, 10 months ago) |
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Multispore takes its time but a month should have been enough time for those grains to have consolidated more thoroughly than that.
I'd say that what's especially worrisome is that you barely have any grain in the jars and yet it hasn't fully colonized.
Wait on a more informed opinion but I'd give that jar a careful whiff before spawning it.
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rustycobwebs
Green mold cultivator

Registered: 12/17/05
Posts: 799
Last seen: 8 years, 9 months
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Re: Healthy mycelium? [Re: Humility]
#14041671 - 02/28/11 12:30 PM (12 years, 10 months ago) |
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Yea a month is a long time for that much grain.
-------------------- and let it be so Praise Psilocybe
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Wise Toad


Registered: 06/08/10
Posts: 2,690
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here is the grow log for the jars, temps weren't great. These jars were inoculated from the last ccs of the syringes I had; 1 or 2 of the syringes had lost a lot of spores when I forgot to shake during previous inoculations which may have made them weak. All of the jars are white, have been easily shaken up, and easily recovered which rules out a lot of types of contams but im still worried about how slow they were
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Blueflowers
Noobin



Registered: 03/15/11
Posts: 174
Loc: Australia
Last seen: 7 days, 8 hours
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Re: Healthy mycelium? [Re: Wise Toad]
#14455077 - 05/15/11 07:09 AM (12 years, 8 months ago) |
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Mmm i have jars like yours that have been colonizing 12 days since ms inoc, though they have only just begun to colonize. let me know how this turns out
cheers
BF
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wygram
Myconaut

Registered: 01/28/07
Posts: 573
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Considering that spores can take 7-10 days to start and the low temperatures that these were growing in, a month isn't a stretch. Especially if they weren't shaken.
On another note, you don't need to let grain jars consolidate before using them for G2G or making grain LCs.
-------------------- Changing your mind is one of the best ways of finding out whether or not you still have one.
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Wise Toad


Registered: 06/08/10
Posts: 2,690
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Re: Healthy mycelium? [Re: wygram]
#14457721 - 05/15/11 05:41 PM (12 years, 8 months ago) |
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What the fuck, somebody resurrected this?
Oh yeah, they turned out fine. They made great GLCs and grew some killer shroomies. They belong to ma belly now
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