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Lifes A Trip
Grown Here Not Flown Here



Registered: 02/22/11
Posts: 52
Loc: The Pacific Northwest
Last seen: 8 years, 3 months
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Frozen GT Syringe
#14035759 - 02/27/11 12:35 PM (12 years, 10 months ago) |
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well my syringe froze in the mailbox for two days when i was out of town, ive heard diffrent opinions on the matter wether the spores are still viable or not but hey i got these jars around and im just gonna go for it. ill post the results.
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lil_demented
Loner will lone

Registered: 09/11/06
Posts: 6,146
Last seen: 1 month, 9 days
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 I think it's going to work.
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Lifes A Trip
Grown Here Not Flown Here



Registered: 02/22/11
Posts: 52
Loc: The Pacific Northwest
Last seen: 8 years, 3 months
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lets hope so
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godisanastronaut
eurofag \o/


Registered: 11/30/10
Posts: 273
Last seen: 11 years, 3 months
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you go ahead and try and tell us if it works. i read that spores in water get destroyed (bc of high pressure or something like that), but maybe its wrong or maybe not all of them get sqeezed to a painful death
--------------------
All things are true. God's an Astronaut. Oz is Over the Rainbow, and Midian is where the monsters live.
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LurkingWizards
WurkingLizards



Registered: 01/19/11
Posts: 219
Loc: hear and now, woof
Last seen: 10 years, 11 months
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they should be fine...
consider this theory... in nature, spore survive the frost of winter and germinate just fine every year... 
good luck and post results for the 'nay-sayers'
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siko887
Broke


Registered: 01/05/11
Posts: 772
Loc: Ontario, Canada
Last seen: 3 years, 3 months
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Quote:
LurkingWizards said: they should be fine...
consider this theory... in nature, spore survive the frost of winter and germinate just fine every year... 
good luck and post results for the 'nay-sayers' 
LurkingWizards types faster than me. But that's what I was gonna say.
-------------------- I don't suffer from insanity, I actually rather enjoy it.
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 4 months
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Quote:
LurkingWizards said: they should be fine...
consider this theory... in nature, spore survive the frost of winter and germinate just fine every year... 
good luck and post results for the 'nay-sayers' 
Chances are they will be fine but lets remember why P. Cubensis doesn't grow up north in the wild.....
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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siko887
Broke


Registered: 01/05/11
Posts: 772
Loc: Ontario, Canada
Last seen: 3 years, 3 months
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Quote:
i GrOw StUFF said:
Quote:
LurkingWizards said: they should be fine...
consider this theory... in nature, spore survive the frost of winter and germinate just fine every year... 
good luck and post results for the 'nay-sayers' 
Chances are they will be fine but lets remember why P. Cubensis doesn't grow up north in the wild.....
That's an untrue statement.
-------------------- I don't suffer from insanity, I actually rather enjoy it.
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 4 months
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Re: Frozen GT Syringe [Re: siko887]
#14036254 - 02/27/11 02:12 PM (12 years, 10 months ago) |
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Well it has been known that they CAN grow north of the freezing line, its pretty rare. Places that experience freezing temps for most of the winter you won't find them....I should do an experiment sometime to see how long P. Cubensis spores can live in a deep freeze....
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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k00laid
NEMO


Registered: 05/03/10
Posts: 19,636
Last seen: 6 months, 5 days
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Re: Frozen GT Syringe [Re: siko887]
#14036260 - 02/27/11 02:13 PM (12 years, 10 months ago) |
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i wouldnt be surprised if the spores were fine.
but water expanding into ice probably compromised the sterility of your syringe.
-------------------- AMU - AMU Q & A - MyVideo Teks!
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siko887
Broke


Registered: 01/05/11
Posts: 772
Loc: Ontario, Canada
Last seen: 3 years, 3 months
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Re: Frozen GT Syringe [Re: k00laid]
#14036281 - 02/27/11 02:20 PM (12 years, 10 months ago) |
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Quote:
k00laid said: i wouldnt be surprised if the spores were fine.
but water expanding into ice probably compromised the sterility of your syringe.
^ This, I suggest doing some agar work. Than you might be able to salvage a clean culture.
-------------------- I don't suffer from insanity, I actually rather enjoy it.
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LurkingWizards
WurkingLizards



Registered: 01/19/11
Posts: 219
Loc: hear and now, woof
Last seen: 10 years, 11 months
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Re: Frozen GT Syringe [Re: siko887]
#14036285 - 02/27/11 02:21 PM (12 years, 10 months ago) |
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Quote:
siko887 said:
Quote:
i GrOw StUFF said:
Quote:
LurkingWizards said: they should be fine...
consider this theory... in nature, spore survive the frost of winter and germinate just fine every year... 
good luck and post results for the 'nay-sayers' 
Chances are they will be fine but lets remember why P. Cubensis doesn't grow up north in the wild.....
That's an untrue statement.
Yeah, is Washington or Oregon far enough north for you? or do we have to go to Canada?
is he thinking of the environmental requirement for cubes to fruit? because they will only propagate in the correct 'hardness' zones, but that has nothing to do will the viability of spores... spores are made to survive harsher conditions than the fruit body, fact.
besides, I highly doubt that the frost will affect the spores of cubes differently than spores of any other species or genera... just my opinion though, so wait for a TC if you feel so inclined
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 4 months
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Yes, Washington is north, but exhibits alot of features that a marine climate would so it doesn't experience the true hard winter. Same with Oregon.....I'm talking about Minnesota and surrounding states, New England area.....Thats what I was referring to by north
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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Morelman
LC Master



Registered: 05/17/06
Posts: 2,625
Loc: Hiding under an Elm Tree
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Re: Frozen GT Syringe *DELETED* [Re: i GrOw StUFF]
#14036443 - 02/27/11 02:53 PM (12 years, 10 months ago) |
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Post deleted by MorelmanReason for deletion: Never again...
Edited by Morelman (02/27/11 03:20 PM)
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dmt005
Stranger

Registered: 12/20/10
Posts: 71
Last seen: 4 years, 3 months
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Quote:
Lifes A Trip said: well my syringe froze in the mailbox for two days when i was out of town, ive heard diffrent opinions on the matter wether the spores are still viable or not but hey i got these jars around and im just gonna go for it. ill post the results.
Im sorry to hear about it mate, however look at the glass half full, this will give you an opportunity to gain some very good personal exp. Just go for it if they work they work if they dont they dont.
-------------------- Mycelium doesn't urinate, so it will never piss red
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LurkingWizards
WurkingLizards



Registered: 01/19/11
Posts: 219
Loc: hear and now, woof
Last seen: 10 years, 11 months
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<-Morelman I should have stayed with the usual UTFSE response instead of trying to answer it myself and get wrapped up in this pointless attempt at guidance... and it's not even with the OP (he and his spores are probably fine and laughing at this whole display of ridiculousness) 
did that guy even read the rest of what I posted about spores in nature?
"true hard winter" wtf so, this guy's spores were frozen for two days... and that makes them worthless?
I think not...
that is not the point I was trying to make anyways... the reason they don't fruit naturally outdoor for you in Minnesota is because the myc. won't grow very well at that low a temp... and that has nothing to do with the spores being able to survive the winter...
In fact, I would bet that you have at least a month of proper climate that's 'good-enough' to fruit a pre-made colonized outdoor bed, wtf does it matter anyways... if they fruit up north on the coasts, it is because they have a 'good-enough' fruiting environment during the spring-fall for the species to recolonize every year, even if it freezes the spores in the winter(hard winter or not)...
tell me you understand the part about spores being able to survive harsher climates than the myc. that's produced??
why am I even arguing this point?... damn you internet 
... I'll just wait for someone else to convince you if that is even the point anymore...
Edited by LurkingWizards (02/27/11 03:07 PM)
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Lifes A Trip
Grown Here Not Flown Here



Registered: 02/22/11
Posts: 52
Loc: The Pacific Northwest
Last seen: 8 years, 3 months
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I've read many of the claims about how they can and cannot work im inoculating today as soon as it UN-thaws haha. in the words of the Great Bob Marley "time will tell." but thank you for the good luck wishes and i believe this will be a learning experience, fail or success, knowledge will be gained!
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Buddaking
Genetics



Registered: 12/18/09
Posts: 1,775
Loc: No Mans Land
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I promise if the was good to start with it will still be good. I have 3 Chilean cakes in a sgfc right now pinning like mad from a syringe that was frozen solid in my refer when some jack hole adjusted the temp to keep his beer colder. I had no intention of fruiting them (I only use brf to test LC & syringes to see if they are good) but I could not bring myself to toss em in the compost either.
--------------------
 AMU Q & A
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Lifes A Trip
Grown Here Not Flown Here



Registered: 02/22/11
Posts: 52
Loc: The Pacific Northwest
Last seen: 8 years, 3 months
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Re: Frozen GT Syringe [Re: Buddaking]
#14036602 - 02/27/11 03:35 PM (12 years, 10 months ago) |
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It is statements like that, that give me a good feeling of a high chance of success, thanks buddaking
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 4 months
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Quote:
LurkingWizards said:
<-Morelman I should have stayed with the usual UTFSE response instead of trying to answer it myself and get wrapped up in this pointless attempt at guidance... and it's not even with the OP (he and his spores are probably fine and laughing at this whole display of ridiculousness) 
did that guy even read the rest of what I posted about spores in nature?
"true hard winter" wtf so, this guy's spores were frozen for two days... and that makes them worthless?
I think not...
that is not the point I was trying to make anyways... the reason they don't fruit naturally outdoor for you in Minnesota is because the myc. won't grow very well at that low a temp... and that has nothing to do with the spores being able to survive the winter...
In fact, I would bet that you have at least a month of proper climate that's 'good-enough' to fruit a pre-made colonized outdoor bed, wtf does it matter anyways... if they fruit up north on the coasts, it is because they have a 'good-enough' fruiting environment during the spring-fall for the species to recolonize every year, even if it freezes the spores in the winter(hard winter or not)...
tell me you understand the part about spores being able to survive harsher climates than the myc. that's produced??
why am I even arguing this point?... damn you internet 
... I'll just wait for someone else to convince you if that is even the point anymore... 
Try to find a place in this thread where I said the syringe would be no good.... ....don't know what a hard winter is? 
"In fact, I would bet that you have at least a month of proper climate that's 'good-enough' to fruit a pre-made colonized outdoor bed, wtf does it matter anyways..." ----- Obviously...this has nothing to do with the subject though...
So Minnesota has a low temp in the summer?? LMAO....Minnesotas average temperature in the summer is around 82 for your info...averages 85 in the south, upper 70's in the north
Yeah your right though, pointless argument
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
Edited by i GrOw StUFF (02/27/11 03:41 PM)
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LurkingWizards
WurkingLizards



Registered: 01/19/11
Posts: 219
Loc: hear and now, woof
Last seen: 10 years, 11 months
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EDiTEd in BOLD...
Quote:
i GrOw StUFF said:
Quote:
LurkingWizards said:
<-Morelman I should have stayed with the usual UTFSE response instead of trying to answer it myself and get wrapped up in this pointless attempt at guidance... and it's not even with the OP (he and his spores are probably fine and laughing at this whole display of ridiculousness) 
did that guy even read the rest of what I posted about spores in nature?
"true hard winter" wtf... this is sarcasm, btw so, this guy's spores were frozen for two days... and that makes them worthless?
I think not... and I'm not trying to say that you think this guy's spores are worthless...
that is not the point I was trying to make anyways... it's spores being able to survive the winter in general...
In fact, I would bet that you have at least a month of proper climate that's 'good-enough' to fruit a pre-made colonized outdoor bed, wtf does it matter anyways... this is also a poor attempt at trying to make you laugh, I know you have the proper temps for outdoor cult... if they fruit up north or on the coasts, it is because they have a 'good-enough' fruiting environment during the spring-fall for the species to recolonize every year, even if it freezes the spores in the winter(hard winter or not)...FACT
tell me you understand the part about spores being able to survive harsher climates than the myc. that's produced??This was the only point I was trying to make, seriously.
why am I even arguing this point?... damn you internet 
... I'll just wait for someone else to convince you if that is even the point anymore... 
Try to find a place in this thread where I said the syringe would be no good.... ....don't know what a hard winter is? 
misinterpreted sarcasm, whoops I didn't say that you posted anything about the syringe being 'no good' either, I was backing up my spores in nature argument and you got all confused spouting about "up north" in the first place
I meant no disrespect... just trying to get my point across, without being misinterpreted...
"In fact, I would bet that you have at least a month of proper climate that's 'good-enough' to fruit a pre-made colonized outdoor bed, wtf does it matter anyways..." ----- Obviously...this has nothing to do with the subject though... sorry, you can't read sarcasm
So Minnesota has a low temp in the summer?? when did I say that? I meant you have too low a temp in the winter for myc to colonizeLMAO....Minnesotas average temperature in the summer is around 82 for your info...averages 85 in the south, upper 70's in the north thanks for the info?
All I was trying to do was help you understand the spore viability issue, lol you brought up the 'north' talk... I guess there is no convincing you that spores can survive harsh conditions, where as the myc requires proper growing conditions to fruit... sorry for all the confusion and misunderstandings
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Lifes A Trip
Grown Here Not Flown Here



Registered: 02/22/11
Posts: 52
Loc: The Pacific Northwest
Last seen: 8 years, 3 months
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inoculated!
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 4 months
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sweet, how many jars?
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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Lifes A Trip
Grown Here Not Flown Here



Registered: 02/22/11
Posts: 52
Loc: The Pacific Northwest
Last seen: 8 years, 3 months
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8 Jars knocked up cant wait to see the mycelium growth!
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Lifes A Trip
Grown Here Not Flown Here



Registered: 02/22/11
Posts: 52
Loc: The Pacific Northwest
Last seen: 8 years, 3 months
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No signs yet, but still waiting patiently
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Lifes A Trip
Grown Here Not Flown Here



Registered: 02/22/11
Posts: 52
Loc: The Pacific Northwest
Last seen: 8 years, 3 months
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still no sign almost six days
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Lifes A Trip
Grown Here Not Flown Here



Registered: 02/22/11
Posts: 52
Loc: The Pacific Northwest
Last seen: 8 years, 3 months
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After 22 days i have seen Growth! only 2 jars showed any mycelium growth i am incubating and the colonization is very slow but ryzimortic growth is occuring in one of the jars. not a total faliure just gonna kick back and let em be. besides i have about 6 or 7 just fruiting the day away in the chamber =)
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Anno
Experimenter




Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 21 days, 14 hours
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Re: Frozen GT Syringe [Re: Morelman]
#14176273 - 03/24/11 02:47 PM (12 years, 10 months ago) |
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Quote:
Morelman said: That being said, Anno did an experiment but he used Jet-Dry in the syringes. Jet-Dry has some sodium and it's mostly citric acid. Which would decrease the temperature at which water will freeze. At least by 10° or more.
I used one drop of it per cup of water, this will have no noticable effect on the freezing point.
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lil_demented
Loner will lone

Registered: 09/11/06
Posts: 6,146
Last seen: 1 month, 9 days
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Now I wonder if they will actually finish. I bet your jars are pretty dried out after 22 days.
Oh yeah, and...
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Morelman
LC Master



Registered: 05/17/06
Posts: 2,625
Loc: Hiding under an Elm Tree
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Re: Frozen GT Syringe *DELETED* [Re: Anno]
#14176562 - 03/24/11 03:27 PM (12 years, 10 months ago) |
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Post deleted by MorelmanReason for deletion: Never again...
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Lifes A Trip
Grown Here Not Flown Here



Registered: 02/22/11
Posts: 52
Loc: The Pacific Northwest
Last seen: 8 years, 3 months
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Re: Frozen GT Syringe [Re: Morelman]
#14185082 - 03/26/11 01:06 AM (12 years, 10 months ago) |
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Good shit ha ha. im hoping ill get something outta them t hada be great
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lil_demented
Loner will lone

Registered: 09/11/06
Posts: 6,146
Last seen: 1 month, 9 days
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Quote:
Lifes A Trip said: Good shit ha ha. im hoping ill get something outta them t hada be great
I bet you get at least enough to get a few prints.
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Lifes A Trip
Grown Here Not Flown Here



Registered: 02/22/11
Posts: 52
Loc: The Pacific Northwest
Last seen: 8 years, 3 months
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thada keep the flow a goin.
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Lifes A Trip
Grown Here Not Flown Here



Registered: 02/22/11
Posts: 52
Loc: The Pacific Northwest
Last seen: 8 years, 3 months
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the two jars are about 80 % colonized only around a week until fc
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