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OfflineCrono
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Registered: 08/09/10
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best or easiest way to clone some reishi?
    #14032730 - 02/26/11 09:06 PM (12 years, 10 months ago)

Alright.  I'm still a noob to this hobby, and pretty broke too, so I want to keep any work as simple and cost effective as possible.  I've been looking around for some magic goodies, but I can't seem to stop stumbling across reishi mushrooms for now.  I won't complain. 

The other day I harvested about 200-250g of fresh PURPLE reishi.  I was very excited when I realized it was not red.  Though there was also some red growing very nearby.  I think these are more rare, they are beautiful anyway.  I'll also be going back to a spot to harvest more red soon.  What is the best way to clone these guys?  I understand two options, a) use a scapel to scratch out some inner tissue, and place this into agar petri dishes.  b) with sterilised water, push a syringe through the tissue, and put this water into liquid culture or agar? 

I could use some help.  Would rather not work with the agar yet if not necessary.  Just not ready to get those supplies.  Is option b) viable?  And how exactly is it done, ie, what do I do with the water once it's all reishi'd up?

thanks


--------------------
“Not being tense but ready.
Not thinking but not dreaming.
Not being set but flexible.
Liberation from the uneasy sense of confinement.
It is being wholly and quietly alive, aware and alert, ready for whatever may come.”  - Bruce Lee



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OfflineCrono
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Registered: 08/09/10
Posts: 54
Loc: South
Last seen: 10 years, 3 months
Re: best or easiest way to clone some reishi? [Re: Crono]
    #14032814 - 02/26/11 09:17 PM (12 years, 10 months ago)

Actually, reconsidering the the agar.  I don't have a flow hood but can build a glovebox with ease.  Would this agar mix work?http://www.hometrainingtools.com/nutrient-agar-sterile-125ml/p/BE-AGARNBT/


--------------------
“Not being tense but ready.
Not thinking but not dreaming.
Not being set but flexible.
Liberation from the uneasy sense of confinement.
It is being wholly and quietly alive, aware and alert, ready for whatever may come.”  - Bruce Lee



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OfflineABC
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Re: best or easiest way to clone some reishi? [Re: Crono]
    #14039267 - 02/27/11 10:21 PM (12 years, 10 months ago)

Tissue to agar will be the most viable. Agar is actually fairly simple. Sometimes you don't even need a hood or glovebox if you work next to a flame, as the upward moving air will prevent thing from landing in your dish. Just work quickly and cleanly and everything will be okay

You'll be better off with PDA or MEA methinks...

Option b is not as viable because:
1) you wont get reliable/sufficient cells for growing by just injecting and sucking up again
2) you can't distinguish contaminant growth in a liquid culture


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OfflineCrono
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Registered: 08/09/10
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Re: best or easiest way to clone some reishi? [Re: ABC]
    #14039493 - 02/27/11 10:58 PM (12 years, 10 months ago)

Thank you for your response.  Makes total sense. 

Edit, gotcha, malt extract and potato dextrose (ie corn sugar) agar.  Seem money friendly to me.

Ok, maybe better luck in front of the oven then.  I just hope I get some to take, they were so pretty the first day picked, and I don't have a camera atm.  I'll update if the news is good.  If not I'll just forget about it and start something new.


--------------------
“Not being tense but ready.
Not thinking but not dreaming.
Not being set but flexible.
Liberation from the uneasy sense of confinement.
It is being wholly and quietly alive, aware and alert, ready for whatever may come.”  - Bruce Lee



Edited by Crono (02/27/11 11:02 PM)


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InvisibleMonkeyKnifeFight
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Registered: 06/08/10
Posts: 772
Re: best or easiest way to clone some reishi? [Re: Crono]
    #14041412 - 02/28/11 11:50 AM (12 years, 10 months ago)

I'd consider building a simple glove box.  I've never heard of working in front of an oven but it doesn't sound ideal.  A simple glove box will let you do all kinds of stuff like grain jars, filter bags etc.


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