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InvisibleExcessiveSamsara
Witnessing
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Registered: 02/21/11
Posts: 36
Questionable LC
    #14030069 - 02/26/11 11:58 AM (12 years, 10 months ago)

it's hard to see in my jars because i may have cooked the honey for too long.  i steamed the jars for 30 minutes, didn't think that would be too excessive.  the two lc's i just did yesterday i steamed for only 25.  i don't feel confident about sterility going below that.

the two that have been going for 8 days now, which i thought were about ready to go, i am now questioning.  i see mycelia growth around the visible spores in the liquid, but i cannot see much other white growth in other areas besides these.  the liquid is yellowish, not very clear, so i'm having trouble seeing what is really in there.

what are the chances i'd get no mycelia sucked up in a 10cc syringe?  i'm looking to inoculate a few pf tek jars today to let grow and see if they contaminate...testing the lc contam status.

should i give it a go?  or do you think i should let it incubate a couple more days until there is more, visible mycelium growth?


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Offlinecyranode
Stranger
Registered: 11/11/10
Posts: 49
Last seen: 9 years, 9 months
Re: Questionable LC [Re: ExcessiveSamsara]
    #14030085 - 02/26/11 12:01 PM (12 years, 10 months ago)

only way to see is to inoculate a jar


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Offlineesquaredx
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Registered: 10/24/09
Posts: 1,497
Loc: Dutch Country
Last seen: 2 years, 4 months
Re: Questionable LC [Re: cyranode]
    #14030250 - 02/26/11 12:34 PM (12 years, 10 months ago)

Give it a try. Swirl it around and then suck some up into your needle.
Goodluck


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InvisibleShr00mZ
Space Invader


Registered: 04/15/08
Posts: 1,454
Re: Questionable LC [Re: esquaredx]
    #14030268 - 02/26/11 12:38 PM (12 years, 10 months ago)

I didnt have my pressure cooker on me one day. So I boiled 2 LC jars for 30 minutes. They are perfectly sterile. Just use very sterile procedures when drawing it out of the jar and placing it in substrate.


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InvisibleExcessiveSamsara
Witnessing
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Registered: 02/21/11
Posts: 36
Re: Questionable LC [Re: Shr00mZ]
    #14030495 - 02/26/11 01:25 PM (12 years, 10 months ago)

i'm going to sterilize the substrate jars tonight and then inoculate from the lc tomorrow.  give it another night.  maybe i'll bring some water from lc up in the syringe and shoot it back in to mix it around more.  see if i get anything good back in the syringe.


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InvisibleDoc_T
Random Dude
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Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Questionable LC [Re: ExcessiveSamsara]
    #14031138 - 02/26/11 04:01 PM (12 years, 10 months ago)

7-14 days to see growth from spores.
Then another 7-14 for colonization.
LC jars take as long as any other jars.


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