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Amazon Shop: ½ Pint Jars, Wild Bird Seed

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OfflineYellowSubmarine
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INNOCULATION: failed?
    #1402682 - 03/23/03 09:44 AM (14 years, 2 months ago)

Hello all. I made my own spore syringe. Have done it in the past with success. I innoculated 6 birdseed quart jars with 5 CCs of spores each. I did that a week ago, put them in my incubator which is kept at a constant 86-88 degrees and there is no sign of anything at all.

what is up here?

thanks


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Offlineshirley knott
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Re: INNOCULATION: failed? [Re: YellowSubmarine]
    #1402756 - 03/23/03 10:22 AM (14 years, 2 months ago)

any time now!


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OfflineYellowSubmarine
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Re: INNOCULATION: failed? [Re: shirley knott]
    #1402830 - 03/23/03 10:52 AM (14 years, 2 months ago)

that doesn't really help me at all - normally when I inoculate I see things happening within three days and that is with only 1 - 2 ccs per jar. now i have 5 ccs, optimal temps and nothing going on --- by the way, right after i injected i shook the jars up to move everything around



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Offlineshirley knott
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Re: INNOCULATION: failed? [Re: YellowSubmarine]
    #1402843 - 03/23/03 10:58 AM (14 years, 2 months ago)

did the spore syringe have time to rehydrate before use?


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OfflineYellowSubmarine
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Re: INNOCULATION: failed? [Re: shirley knott]
    #1402854 - 03/23/03 11:02 AM (14 years, 2 months ago)

yeah they were in their syringes for about 30 hours before going into the jars -


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Offlineshirley knott
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Re: INNOCULATION: failed? [Re: YellowSubmarine]
    #1402865 - 03/23/03 11:06 AM (14 years, 2 months ago)

how lonng jars sterilised for?


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OfflineYellowSubmarine
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Re: INNOCULATION: failed? [Re: shirley knott]
    #1403043 - 03/23/03 12:20 PM (14 years, 2 months ago)

jars were sterilized, 12 - 16 hours innoculated..


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Offlinesi1988crx
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Re: INNOCULATION: failed? [Re: YellowSubmarine]
    #1407323 - 03/25/03 02:31 AM (14 years, 2 months ago)

You may have added too much water and thrown off the moisture content of the wbs. Also, growing mycelium puts off its own heat (a few degrees) so putting it in a incubator at 88 degrees might cook the insides of the jars.


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OfflineAnnoA
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Re: INNOCULATION: failed? [Re: YellowSubmarine]
    #1407331 - 03/25/03 02:38 AM (14 years, 2 months ago)

Inoculation
inoculated
NOT with double NN


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Amazon Shop: ½ Pint Jars, Wild Bird Seed

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