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Offlinesiko887
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Cloning vs. Isolating from MS
    #14025317 - 02/25/11 12:45 PM (12 years, 11 months ago)

So I'm about to start some agar work soon; as soon as my petri dishes arrive in the mail. I was wondering which route I should go. Should I clone a nice looking fruit to agar than make an lc after I have a clean culture. Or should I create a culture from MS on agar than than noc up test jars to see how the strain fruits. It just seems like a hit and miss thing when doing it from MS. As for cloning I'm under the impression that the cloned fruits will share the same attributes as its parent fruit did. Also is there any other benefits to either one that I don't know about?


Correct me if I'm wrong and feel free to leave your opinion. Would like to get a large amount of opinions.

Thanks in advance.


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Re: Cloning vs. Isolating from MS [Re: siko887]
    #14025514 - 02/25/11 01:30 PM (12 years, 11 months ago)

Isolating from spores gives you a broader pool of candidate gene lines, increasing your odds of finding something extraordinary.
Tissue clones will generally contain multiple substrains, and it's possible one or more of those is a star too.

Well-domesticated lines have pretty homogenous spore genetics, there's nowhere near as much variation as with a wild specimen.

My advice: do both.


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