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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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Infected dish: there just isn't a way....or is there?
#14023453 - 02/25/11 12:20 AM (13 years, 9 days ago) |
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Hello,
I have a three section petri that has no bacterial contams showing, and strong growth in all three sectors.
...and a cute little trich blob rocking in one sector as well.

In considering what to do with this dish I can only conclude to just chuck it without ever trying to do a transfer.
I cannot open it in front of my flow hood....the trich is sporulating.
Also, I cannot open it anywhere else if I want to trust any transfers from it.
I suppose that I could open it somewhere else and drop some bleach onto the trich, then move back in front of the hood.
This question is academic, as I will use the dish that these were transferred from to make new transfers.
It just seems a shame.
Take care,
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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Larrythescaryrex
teardrop on the fire



Registered: 07/19/00
Posts: 11,004
Loc: further down the spiral
Last seen: 1 year, 2 months
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Re: Infected dish: there just isn't a way....or is there? [Re: Javadog]
#14023474 - 02/25/11 12:23 AM (13 years, 9 days ago) |
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I would open it in a still air glove box and remove a wedge from one of the other sections to a new dish.
-------------------- RIP Acidic_Sloth Sunset_Mission said: "larry the scary rex verily scary when thoroughly vexed invoke the shadows and dust, cast a hex mercifully massacring memories masterfully relocate from Ur to 8th density and become a cosmic bully mulder and scully couldn't decipher his glyphs invoke the shadows and dust, smoke infernal spliffs" April 24th 2011
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k00laid
NEMO


Registered: 05/03/10
Posts: 19,636
Last seen: 7 months, 6 days
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Re: Infected dish: there just isn't a way....or is there? [Re: Larrythescaryrex]
#14023844 - 02/25/11 02:27 AM (13 years, 8 days ago) |
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^ still air box it.
-------------------- AMU - AMU Q & A - MyVideo Teks!
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hamloaf
Pork Block



Registered: 12/23/09
Posts: 20,584
Loc: Oklahoma.
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Re: Infected dish: there just isn't a way....or is there? [Re: Javadog]
#14024160 - 02/25/11 06:46 AM (13 years, 8 days ago) |
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Use the hot agar pour.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 12 days
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Re: Infected dish: there just isn't a way....or is there? [Re: Javadog]
#14024273 - 02/25/11 07:51 AM (13 years, 8 days ago) |
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Looks more like cladisporium than trich.
Mold is to be expected on agar. That's why we use it in the first place. Simply run your flowhood for an hour or so and then shut it off and do your transfer of healthy mycelium to new plates. Let it grow for two or three days and then grab mycelium again and transfer it to a third set of plates before the mold has a chance to grow. This is the reason we use petri dishes. Moldy and/or bacteria infested prints can easily be cleaned up on agar. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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Re: Infected dish: there just isn't a way....or is there? [Re: RogerRabbit]
#14024546 - 02/25/11 09:28 AM (13 years, 8 days ago) |
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Well, I had forgotten my SAB....I have not used it in about eight months.
I think that I will try RR's approach, just as an experiment.
Thanks team!
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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hamloaf
Pork Block



Registered: 12/23/09
Posts: 20,584
Loc: Oklahoma.
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Re: Infected dish: there just isn't a way....or is there? [Re: Javadog]
#14029659 - 02/26/11 10:15 AM (13 years, 7 days ago) |
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Surprised to see RR prescribe open air procedures, especially with agar. I didn't know you can do that.
Although work intensive, a hot agar pour, IMHO, is the safest way to transfer healthy mycelium from contaminates. Using the hot agar pour, if you catch the mushroom mycelium before the contaminate busts through the surface of the second layer of agar, healthy mycelium can be transferred from contaminates safely in front of a flowhood. What is the strain you have on the agar?
Take care and Good luck, JD.
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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Re: Infected dish: there just isn't a way....or is there? [Re: hamloaf]
#14029679 - 02/26/11 10:23 AM (13 years, 7 days ago) |
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Thanks Hamloaf.
Would you believe....this is me, fighting on, with the Jalisco.
(PM for a dish, as fruits seem a ways off...LOL!)
Take care,
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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