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InvisibleJavadog
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Infected dish: there just isn't a way....or is there?
    #14023453 - 02/25/11 12:20 AM (13 years, 9 days ago)

Hello,

I have a three section petri that has no bacterial contams showing,
and strong growth in all three sectors.

...and a cute little trich blob rocking in one sector as well.



In considering what to do with this dish I can only conclude to just
chuck it without ever trying to do a transfer.

I cannot open it in front of my flow hood....the trich is sporulating.

Also, I cannot open it anywhere else if I want to trust any transfers from it.

I suppose that I could open it somewhere else and drop some bleach onto
the trich, then move back in front of the hood.

This question is academic, as I will use the dish that these were transferred
from to make new transfers.

It just seems a shame.

Take care,

JD


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OfflineLarrythescaryrex
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Re: Infected dish: there just isn't a way....or is there? [Re: Javadog]
    #14023474 - 02/25/11 12:23 AM (13 years, 9 days ago)

I would open it in a still air glove box and remove a wedge from one of the other sections to a new dish.


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Offlinek00laid
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Re: Infected dish: there just isn't a way....or is there? [Re: Larrythescaryrex]
    #14023844 - 02/25/11 02:27 AM (13 years, 8 days ago)

^ still air box it.


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Invisiblehamloaf
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Re: Infected dish: there just isn't a way....or is there? [Re: Javadog]
    #14024160 - 02/25/11 06:46 AM (13 years, 8 days ago)

Use the hot agar pour.


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OfflineRogerRabbitM
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Re: Infected dish: there just isn't a way....or is there? [Re: Javadog]
    #14024273 - 02/25/11 07:51 AM (13 years, 8 days ago)

Looks more like cladisporium than trich.

Mold is to be expected on agar.  That's why we use it in the first place.  Simply run your flowhood for an hour or so and then shut it off and do your transfer of healthy mycelium to new plates.  Let it grow for two or three days and then grab mycelium again and transfer it to a third set of plates before the mold has a chance to grow.  This is the reason we use petri dishes.  Moldy and/or bacteria infested prints can easily be cleaned up on agar.
RR


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InvisibleJavadog
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Re: Infected dish: there just isn't a way....or is there? [Re: RogerRabbit]
    #14024546 - 02/25/11 09:28 AM (13 years, 8 days ago)

Well, I had forgotten my SAB....I have not used it in about eight months.

I think that I will try RR's approach, just as an experiment.

Thanks team!

JD


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Invisiblehamloaf
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Re: Infected dish: there just isn't a way....or is there? [Re: Javadog]
    #14029659 - 02/26/11 10:15 AM (13 years, 7 days ago)

:shocked:

Surprised to see RR prescribe open air procedures, especially with agar.  I didn't know you can do that.

Although work intensive, a hot agar pour, IMHO, is the safest way to transfer healthy mycelium from contaminates.  Using the hot agar pour, if you catch the mushroom mycelium before the contaminate busts through the surface of the second layer of agar, healthy mycelium can be transferred from contaminates safely in front of a flowhood.  What is the strain you have on the agar?



Take care and Good luck, JD.


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InvisibleJavadog
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Re: Infected dish: there just isn't a way....or is there? [Re: hamloaf]
    #14029679 - 02/26/11 10:23 AM (13 years, 7 days ago)

Thanks Hamloaf.

Would you believe....this is me, fighting on, with the Jalisco.

(PM for a dish, as fruits seem a ways off...LOL!)

Take care,

JD


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