|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Zelse
Now with more Vitamin P!


Registered: 01/26/11
Posts: 205
|
Mini-fridge for storing cultures on agar.
#14015723 - 02/23/11 07:01 PM (13 years, 9 days ago) |
|
|
I'd like to start out by saying that I enjoy mycology strictly as a hobby, and have no desire to produce for profit (illegal or otherwise) so I don't need and amazing/expensive professional setup.
I've been toying with the idea of strain isolation/cloning, and I was wondering if cultures in a dish would keep for a while if stored in one of those 6-can mini fridges they sell. Also is there any way to store a master culture semi-permanently?
I tried the search function, but after 2 search terms and 6 total pages of browsing, no luck, so I figured I'd just ask.
Thanks in advance.
-------------------- Feed my will to feel this moment, urging me to cross the line. Reaching out to embrace the random. Reaching out to embrace whatever may come. Taking the Plunge: My First Trip The Tao te Ching
|
RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 12 days
|
Re: Mini-fridge for storing cultures on agar. [Re: Zelse] 1
#14017015 - 02/23/11 10:38 PM (13 years, 9 days ago) |
|
|
You want a fairly stable temperature for long term storage or the slants will develop condensation inside, ruining the cultures.
I have a $199 Sears mini-fridge and it works OK, but oscillates by a few degrees, so I keep the cultures in insulated lunch boxes, which are then placed in the fridge. The insulated lunch boxes keep the slants at a stable temperature. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
Zelse
Now with more Vitamin P!


Registered: 01/26/11
Posts: 205
|
Re: Mini-fridge for storing cultures on agar. [Re: RogerRabbit]
#14017469 - 02/24/11 12:00 AM (13 years, 9 days ago) |
|
|
Thanks a lot! I really appreciate the help. Great videos too.
-------------------- Feed my will to feel this moment, urging me to cross the line. Reaching out to embrace the random. Reaching out to embrace whatever may come. Taking the Plunge: My First Trip The Tao te Ching
|
MycoAu
5thKingdomCome


Registered: 07/18/07
Posts: 1,047
Last seen: 3 years, 2 months
|
Re: Mini-fridge for storing cultures on agar. [Re: Zelse]
#14019831 - 02/24/11 12:51 PM (13 years, 9 days ago) |
|
|
RR, what temperature do you set the fridge to? And aside from Volvariella species, are there any specific species that you exclude from storage in this manner?
|
Lennybernadino
Amazon grower


Registered: 09/16/09
Posts: 770
Loc: Iquitos, Peru
Last seen: 29 days, 2 hours
|
Re: Mini-fridge for storing cultures on agar. [Re: MycoAu]
#14020002 - 02/24/11 01:30 PM (13 years, 8 days ago) |
|
|
Great tip RR! That will really help. jEr
|
mycoelf
Agent Of Chaos



Registered: 06/26/09
Posts: 555
Loc: hyperspace
|
Re: Mini-fridge for storing cultures on agar. [Re: Lennybernadino]
#14021929 - 02/24/11 07:19 PM (13 years, 8 days ago) |
|
|
42 deg f
-------------------- Mycoelf Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable the goal of infinity becomes. Remember, cleanliness in next to goddessness
      
|
RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 12 days
|
Re: Mini-fridge for storing cultures on agar. [Re: mycoelf] 1
#14023288 - 02/24/11 11:45 PM (13 years, 8 days ago) |
|
|
I thought I replied to this earlier, but I see my post isn't here. I keep the cultures just above freezing, 1C to 2C. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
|