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Offlinekushking420
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Registered: 02/24/11
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inoculated 6 days ago sour smell
    #14019424 - 02/24/11 11:23 AM (12 years, 11 months ago)

Ok, so first of all I would like to say that I'm new to this, but I have been doing alot of research. I'll explain everything from the beginning. I've inoculated 6 six widemouth half pint BRF/verm jars six days ago with Mexi-Cub- Psilocybe Cubensis spores ordered from a reliable site, now when I put my nose up to the holes it has a very nasty sour smell. The jars were also ordered off the internet and guaranteed sterile upon arrival. They came wrapped in alluminum foil with microtape over the 4 holes.

I was pretty sure to be sterile, i wore a mask, made sure i was in a no draft area, AC was off windows shut, I disinfected the table I used, poured alcohol all over my hands and arms multiple times throughout the procedure. The needle was sterile for the first jar, but after the first jar I just flame sterilized the tip. It turned black, but I didn't wipe it off. I dont know maybe this is the reason. no sign of colonization really.

Any ideas? hope they're not contamed

also some of the jars may have like 2-3 CC is them because the syinge would get stuck then I'd have to push harder causing alot to come out at once.


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InvisibleEvilMushroom666
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Re: inoculated 6 days ago sour smell [Re: kushking420]
    #14019440 - 02/24/11 11:26 AM (12 years, 11 months ago)

In my experience sour smells, reminiscent of dirty socks, rotting fruit,
sweet funk, etc in BRF cakes within the first week usually means you
have bacterial contaminates.

1CC is more then enough per jar, any more can throw off the water
content of your jars resulting in bacterial and other contaminant
growths.

Also was this "reliable" site a Shroomery sponsor?


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Offlinekushking420
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Re: inoculated 6 days ago sour smell [Re: EvilMushroom666]
    #14019513 - 02/24/11 11:43 AM (12 years, 11 months ago)

No I don't think so...I dont know whether I'm allowed to say this but the spores were ordered off of spores101 the jars were from ebay.......


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Offlinekushking420
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Re: inoculated 6 days ago sour smell [Re: EvilMushroom666]
    #14019525 - 02/24/11 11:44 AM (12 years, 11 months ago)

also, just to let you know they don't smell unless I put my nose up to it. One of the jars doesn't even smell. I would have never known if I didn't put my nose up to it. but it really does smell nasty I'm going to post pictures soon.


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InvisibleEvilMushroom666
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Re: inoculated 6 days ago sour smell [Re: kushking420]
    #14019549 - 02/24/11 11:49 AM (12 years, 11 months ago)

If it smells as nasty as you state, give it another day or two and
the area that you keep your jars in will stink. Spores 101 is notorious
for selling bunk syringes as well as plain water syringes, google
spores101 reviews, or hit the search engine.

Next time get your spores from a Sponsor, and make your own BRF jars.

http://www.mushroomvideos.com/BRF-Pf-Tek

I personally would toss out any jars that had a funky smell coming
from them. 100% of the time in my experience it is bacterial contamination.


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Offlinekushking420
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Re: inoculated 6 days ago sour smell [Re: EvilMushroom666]
    #14019597 - 02/24/11 12:02 PM (12 years, 11 months ago)

Ok thanks for your feedback. Here are some pictures, let me know what you think. Keep in mind it is 6 days from inoc and has been kept in room temp which varies from 68-76 degrees. but is usually around 73.







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Offlinemagnumzero
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Re: inoculated 6 days ago sour smell [Re: kushking420]
    #14019636 - 02/24/11 12:13 PM (12 years, 11 months ago)

Bacteria. Toss and order from sporeworks, hawkseye, or ralphsters. Also, making your own jars is really easy.

I  know why your syringe got stuck. Verm can clog the neddle. It takes practice but eventually you'll figure out how to work the syringe delicately.


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Don't use incubators. An incubator is for contamination as brothels are for STDs. Sure you get sex, but is it worth it? :shrug:


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Offlinekushking420
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Re: inoculated 6 days ago sour smell [Re: magnumzero]
    #14019708 - 02/24/11 12:28 PM (12 years, 11 months ago)

alright I guess I will throw them out and properly start over...how should I go about doing this......Can I just throw out the mixture and wash the jars, or do I have to kill the bacteria before opening the jars........i want to use these jars for a future grow. thank you


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Offlinetibberous
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Re: inoculated 6 days ago sour smell [Re: magnumzero]
    #14019774 - 02/24/11 12:40 PM (12 years, 11 months ago)

Not sure I'd trust the eBay jars...

And spores101 has never sent me a bad syringe, while some sponsor vendors have. Ever vendor is going to send out a bad syringe once in a while - could be that.

You'd have to REALLY fuck up the inoculation to contaminate EVERY jar.

Nothing you can do with them at this point but wait. While your waiting, I'd order a couple more syringes, get a PC and make some jars myself.

You could go to walmart right now, spend $40 on a PC, $10 on jars, $5 on brown rice, $2 on aluminum foil and $2 on stickers and have 12 GOOD jars, and most the shit you'd need for next time. That's $60 + spores, and a few hours making them.


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Offlinetibberous
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Re: inoculated 6 days ago sour smell [Re: tibberous]
    #14019814 - 02/24/11 12:47 PM (12 years, 11 months ago)

!!!

Don't throw them out!

Shit, it's only 6 days - some might be fine. Give it a couple weeks unless they visibly show mold or stink so damn bad you can't take it.

There might just be a couple bad jars, or maybe none are bad and your just smelling wet brown rice flour.

That's why it's better to have a lot of jars going - it decreases the amount of time you can obsess over and fuck with any single jar :smile:


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Offlinemagnumzero
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Re: inoculated 6 days ago sour smell [Re: tibberous]
    #14019825 - 02/24/11 12:49 PM (12 years, 11 months ago)

"And spores101 has never sent me a bad syringe, while some sponsor vendors have. "
Thing is, sponsors will replace contaminated syringes. Other vendors may not.

And he doesn't need a pressure cooker for PF tek. Skip the PC for now, as long as you have a large pot you can steam sterilize in. Save your money for a high-quality PC. I spent a good amount on my AA921 and it was WELL WORTH IT.

Oh my god, I can only imagine the headaches and time and even money that extra $$ investment saved me..


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Don't use incubators. An incubator is for contamination as brothels are for STDs. Sure you get sex, but is it worth it? :shrug:


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Offlinemagnumzero
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Re: inoculated 6 days ago sour smell [Re: magnumzero]
    #14019828 - 02/24/11 12:50 PM (12 years, 11 months ago)

Tib--you don't see orange splotches in his jars at the inoc points?
It looks like a contam.


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Trade List!
Don't use incubators. An incubator is for contamination as brothels are for STDs. Sure you get sex, but is it worth it? :shrug:


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InvisibleEvilMushroom666
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Re: inoculated 6 days ago sour smell [Re: magnumzero]
    #14019871 - 02/24/11 12:59 PM (12 years, 11 months ago)

If it smells as bad as OP has stated I guarantee it to be bacterial
contaminates. I had a lot of experience with bacterial contaminates
and BRF jars for oysters when I first got into growing edibles, if it
smells foul, sweet, like dirty socks, body odor, rotting fruit, etc,
it almost 100% without a doubt is bacteria.


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Offlinekushking420
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Re: inoculated 6 days ago sour smell [Re: EvilMushroom666]
    #14020064 - 02/24/11 01:43 PM (12 years, 11 months ago)

thanks for help guys....i mean the smell kind of reminds me of a salty sour kind of smell......but like i said before i would have never know. i had to put my nose right up to the holes in the lids to smell it. you can see in the pictures above...the areas where i inoculated are kind of orangish.


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Offlinekushking420
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Re: inoculated 6 days ago sour smell [Re: kushking420]
    #14020080 - 02/24/11 01:46 PM (12 years, 11 months ago)

im going to leave them for a few more days and let you know what happens. Ill post new pics in a couple of days.


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Offlinemagnumzero
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Re: inoculated 6 days ago sour smell [Re: kushking420]
    #14020092 - 02/24/11 01:50 PM (12 years, 11 months ago)

Anyway, after they contaminate some more steam sterilize them before opening outdoors.


--------------------

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Don't use incubators. An incubator is for contamination as brothels are for STDs. Sure you get sex, but is it worth it? :shrug:


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Invisiblevirus1824
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Re: inoculated 6 days ago sour smell [Re: magnumzero]
    #14020100 - 02/24/11 01:52 PM (12 years, 11 months ago)

sour smell=throw. You can wait a few days but im quite sure it'l end up in throw since it wont go away.

good luck


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Offlinetibberous
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Re: inoculated 6 days ago sour smell [Re: magnumzero]
    #14020634 - 02/24/11 03:38 PM (12 years, 11 months ago)

Quote:

magnumzero said:
Thing is, sponsors will replace contaminated syringes. Other vendors may not.





Wow, really? I didn't know that.

I'm not arguing that the jars aren't shot, just saying you don't really gain anything by racing to toss them out (it isn't like green/black mold that's going to spread / hurt you)

If they still look bad in a couple days, toss em.


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InvisibleRafiikii
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Re: inoculated 6 days ago sour smell [Re: tibberous]
    #14020664 - 02/24/11 03:46 PM (12 years, 11 months ago)

gnarsty


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Offlinemagnumzero
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Re: inoculated 6 days ago sour smell [Re: Rafiikii]
    #14020944 - 02/24/11 04:43 PM (12 years, 11 months ago)

Quote:


Wow, really? I didn't know that.




Yeah, the trick is telling them it's contaminated without revealing that you did more than look at it under your microscope.


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Don't use incubators. An incubator is for contamination as brothels are for STDs. Sure you get sex, but is it worth it? :shrug:


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