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Invisiblefngbronco
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Registered: 09/26/10
Posts: 2,877
Losing the battle with bacrteria
    #14018052 - 02/24/11 02:32 AM (13 years, 9 days ago)

Every time I try making a grain jar I've got bacterial infections within a week. My oyster jar made it 75% before it stalled. I'm washing, soaking, simmering, pc'ing 15-20 psi for 60-90 minutes, but within a week they stink. I've got Tyvek lids on some and regular on others. Any advice would be greatly appreciated. There isn't excessive water most the time, but the top 1" dries out pretty signficantly as well.

Could I be cooking to hot or long? Are the grains not soaked through enough? I don't have many burst grains but they're kinda hard when they go in.


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I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

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Offlinechains


Registered: 01/31/11
Posts: 323
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Re: Losing the battle with bacrteria [Re: fngbronco]
    #14018242 - 02/24/11 04:14 AM (13 years, 9 days ago)

Ive been having a few probs with my popcorn jars lately as well. Gettin about 30% contam in my jars to Bacilus. I cant figure out why either! I soak and pc just like you...


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OfflineProfessorPinHead
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Re: Losing the battle with bacrteria [Re: fngbronco]
    #14018243 - 02/24/11 04:16 AM (13 years, 9 days ago)

This is to both you guys.

Are they wide mouth jars?

If so are the rings and lids used?

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Offlinechains


Registered: 01/31/11
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Re: Losing the battle with bacrteria [Re: ProfessorPinHead]
    #14018249 - 02/24/11 04:22 AM (13 years, 9 days ago)

Mine arent wide mouth and just have regular lids with a whole and polyfil for a filter.


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OfflineProfessorPinHead
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Re: Losing the battle with bacrteria [Re: chains]
    #14018284 - 02/24/11 04:47 AM (13 years, 9 days ago)

Have they been used a couple of times. Sometimes the rings get warped and it can leave small gaps and shit can get into the jars after pc'ing. If you hold the bad jar and run your finger in a circle around the rim of the lid and feel a warble in it then that is likely the issue.

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InvisibleDoc_T
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Re: Losing the battle with bacrteria [Re: ProfessorPinHead]
    #14018485 - 02/24/11 06:30 AM (13 years, 9 days ago)

Bad syringe. Agar it.


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InvisibleSillicybin
Registered: 02/14/05
Posts: 2,134
Loc: Flag
Re: Losing the battle with bacrteria [Re: Doc_T]
    #14018521 - 02/24/11 06:49 AM (13 years, 9 days ago)

Quote:

Doc_T said:
Bad syringe. Agar it.




This.  Also, make sure you're PCing for 90 minutes (you said 60 to 90, but go for 90.)  A lot of people get away with 60, but especially if you're not at sea level, the extra time can be necessary.

If you don't have any agar on hand, sterilize a test jar that you do not inoculate and see if it contaminates.  That will isolate your sterilization procedure from your inoculum so you know where your point of failure lies.

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OfflineThe_Outsider
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Re: Losing the battle with bacrteria [Re: Sillicybin]
    #14018613 - 02/24/11 07:35 AM (13 years, 9 days ago)

Quote:

Sillicybin said:
Quote:

Doc_T said:
Bad syringe. Agar it.




This.  Also, make sure you're PCing for 90 minutes (you said 60 to 90, but go for 90.)  A lot of people get away with 60, but especially if you're not at sea level, the extra time can be necessary.

If you don't have any agar on hand, sterilize a test jar that you do not inoculate and see if it contaminates.  That will isolate your sterilization procedure from your inoculum so you know where your point of failure lies.




yup 90 minutes. Its been said time and time again. 60 is flirting with disaster. Why chump yourself when you have already done all the work?


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Offlinetripdawg420
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Re: Losing the battle with bacrteria [Re: The_Outsider]
    #14018754 - 02/24/11 08:36 AM (13 years, 9 days ago)

http://www.shroomery.org/forums/showflat.php/Number/11525679 i have used this tek prolly 70 times perfect every time just use hot water thru the whole tek but it could be any thing just use sponsers at first for yur syringes so u can eliminate alot of shit that could go wrong then learn how they do it ya know well good luck


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How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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Invisibleconformist
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Registered: 07/29/10
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Re: Losing the battle with bacrteria [Re: fngbronco]
    #14018835 - 02/24/11 08:58 AM (13 years, 9 days ago)

How are you inoculating you grain? Are you shooting spores through a port in the lid or opening the lid to put them in? Are you putting a piece of colonized agar in the jar?

Are you using a flowhood or Still air Box of some sort while inoculating or are you doing it in open air?

Leave a jar untouched on your next batch and see if it gets contaminated.

I'm sure you'll find the problem.

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Offlinechains


Registered: 01/31/11
Posts: 323
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Re: Losing the battle with bacrteria [Re: ProfessorPinHead]
    #14018988 - 02/24/11 09:40 AM (13 years, 9 days ago)

Quote:

ProfessorPinHead said:
Have they been used a couple of times. Sometimes the rings get warped and it can leave small gaps and shit can get into the jars after pc'ing. If you hold the bad jar and run your finger in a circle around the rim of the lid and feel a warble in it then that is likely the issue.



:thumbup:

Yup think its my old jars that are the problem. Ive got contams from agar and from g2g but my aseptic technique's spot on so think its time for me to get new jars.


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OfflineRogerRabbitM
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Re: Losing the battle with bacrteria [Re: conformist]
    #14018996 - 02/24/11 09:42 AM (13 years, 9 days ago)

If the top 1" of grains are drying out, then you don't have a proper lid.  A properly made grain jar won't dry out for six to nine months.  Tyvek is a piss-poor filter at best for jars, and should never be used as a 'lid'.  Post Office tyvek is even worse than coverall tyvek, but neither hold up like synthetic filter disks.

The most common cause of bacteria in grain jars is the breath and/or dirty fingernails of the cultivator.  I suspect you're failing to use gloves and a surgical(not dust) mask.
RR


--------------------
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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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InvisibleDoc_T
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Re: Losing the battle with bacrteria [Re: chains]
    #14019014 - 02/24/11 09:45 AM (13 years, 9 days ago)

Quote:

chains said:
Yup think its my old jars that are the problem. Ive got contams from agar and from g2g but my aseptic technique's spot on so think its time for me to get new jars.




New lids, like RR said. There's nothing wrong with your jars.
(I like polyfil better than tyvek. Haven't tried the filter disks yet.)


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Offlineskwerlee
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Registered: 02/22/11
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Re: Losing the battle with bacrteria [Re: Doc_T]
    #14019033 - 02/24/11 09:50 AM (13 years, 9 days ago)

Just a quick thought.. after you put your sub into the jar, before you put your verm barrier on the top. Are you wiping all your edges clean? I have heard of contam happening this way.

better luck in the future mate!

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Offlinesiko887
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Re: Losing the battle with bacrteria [Re: skwerlee]
    #14019120 - 02/24/11 10:09 AM (13 years, 9 days ago)

Quote:

skwerlee said:
Just a quick thought.. after you put your sub into the jar, before you put your verm barrier on the top. Are you wiping all your edges clean? I have heard of contam happening this way.

better luck in the future mate!



These are grain jars. What are you talking about with a dry verm layer? Grain jars have a completely different filter system that normally use polyfill, tyvek or filter discs. There should be no vermiculite introduced at all during this stage.


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Invisiblefngbronco
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Re: Losing the battle with bacrteria [Re: skwerlee]
    #14019161 - 02/24/11 10:21 AM (13 years, 9 days ago)

It's been happening on both my widemouth and regular mouth quart jars. No gloves or mask but tons of alcohol and flame sterilizing. My pf style jars colonize better but lately they've been stalling with contams as well. I'm using sponsor cultures. The Tyvek will be destroyed and plastic lids will be purchased. I have 7 filter disks and tons of polyfil as well. How large of a hole would you recommend in a plastic lid and what size and sealant for an injection port? Ally liquid cultures and agar have been getting bacteria as well. The agar is all done in a glovebox that's been sprayed and sealed until use. I was using cattle insemination gloves but have Tyvek sleeves en route. The bacteria colonies look like opaque spots of slime on agar. This has happened on all my grain attempts whether it be WBS or rye or tertricalle (sp?). I do have N95 masks and a box of nitriles I will start wearing when innoculating jars, but most don't even make it that far.


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I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek

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InvisibleDoc_T
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Re: Losing the battle with bacrteria [Re: fngbronco]
    #14019167 - 02/24/11 10:22 AM (13 years, 9 days ago)

I use 1/4" holes in lids, seems to work ok.


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OfflineRogerRabbitM
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Re: Losing the battle with bacrteria [Re: fngbronco]
    #14019244 - 02/24/11 10:40 AM (13 years, 9 days ago)

Quote:

fngbronco said:
It's been happening on both my widemouth and regular mouth quart jars. No gloves or mask but tons of alcohol and flame sterilizing.



Bingo.  Bacteria problem solved.  You should be using alcohol and flame sterilization, a flow hood, etc., but gloves and mask are extremely important.

Quote:

fngbronco said:The Tyvek will be destroyed and plastic lids will be purchased. I have 7 filter disks and tons of polyfil as well.




Plastic lids suck.  Get metal lids, drill four 1/16" to 1/8" holes in each lid.  Place the lid down, then the filter disk, and then screw the ring on top.  This keeps your filter disk dry.  If you insist on using a syringe, lift the lid and filter up and squirt the spores directly in, obviously in a glovebox or preferably in front of a laminar flow hood.

Quote:

fngbronco said:I do have N95 masks and a box of nitriles.




If they're N95 dust masks, they're useless.  They're designed to protect the wearer from contaminants entering his respiratory system.  They do nothing to filter the bad breath of the wearer.  Surgical masks on the other hand are designed to stop 99% of the exhaled bacteria from the doctor, thus protecting the patient.  This is what we should be using.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Invisiblefngbronco
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Re: Losing the battle with bacrteria [Re: RogerRabbit]
    #14019272 - 02/24/11 10:47 AM (13 years, 9 days ago)

They're the medical N95 masks. I will start to go under the lid rather than through it from here on out. How should I inoculate ones with polyfil (no money for a while) and how tight should it be placed in the holes?


--------------------
I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek

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Invisibleheeroyuy00
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Registered: 09/27/09
Posts: 299
Re: Losing the battle with bacrteria [Re: fngbronco]
    #14019429 - 02/24/11 11:24 AM (13 years, 9 days ago)

i used that heat gasket injection port tek with tyvek and ever since i switched to wbs jars ive been 100%

even when i have extra spawn i close the lid up and it stays clean some how .. i dont know

but rr is probably right get some new jars and a better filter



and sea level is a factor? ... im below sea level whats that mean ?
also bad needle


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Good Links

Edited by heeroyuy00 (02/24/11 11:26 AM)

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