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Offlinecaricapapaya
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Re: Micro propagation at home. [Re: Burbles]
    #14068462 - 03/04/11 09:41 PM (12 years, 10 months ago)

yes, put sucrose in everything. I am sure there is a protocol for something that doesnt use it, but for the most part, use it.


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OfflineBurblesV
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Re: Micro propagation at home. [Re: caricapapaya]
    #14072118 - 03/05/11 06:06 PM (12 years, 10 months ago)

Here is some info on various OTC sources of plant hormones...

http://www.thelaboratory.org/talk/index.php?topic=33.0
Including extracting gibberlic acid from seeds, and a few other good places..


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InvisibleDoc_T
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Re: Micro propagation at home. [Re: Burbles]
    #14073259 - 03/05/11 10:25 PM (12 years, 10 months ago)

You're coming at this from the wrong end.
We don't links to how to make hormones.

We need to know which hormones, if any, are required for this to work.
THEN we can learn how to make them. You're confusing data with information, you've been doing it all through this thread.
There's several people that have posted really irrelevant links.


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You make it all possible. Doesn't it feel good?


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Invisiblecacharstar
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Re: Micro propagation at home. [Re: cacharstar]
    #14073331 - 03/05/11 10:42 PM (12 years, 10 months ago)

Quote:

cacharstar said:



I found this research done on the best adjustment of the ms medium for hemp but am having trouble understanding exactly how to carry out the mixing without a firm background in chem.. Perhaps we can help each other.:rasta: Here is the linkhttp://www.pakbs.org/pjbot/PDFs/41%282%29/PJB41%282%29603.pdf
.




--------------------
:irishtoast:


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OfflineBurblesV
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Re: Micro propagation at home. [Re: Doc_T]
    #14074659 - 03/06/11 08:46 AM (12 years, 10 months ago)

Quote:

Doc_T said:
You're coming at this from the wrong end.
We don't links to how to make hormones.

We need to know which hormones, if any, are required for this to work.
THEN we can learn how to make them. You're confusing data with information, you've been doing it all through this thread.
There's several people that have posted really irrelevant links.





I thought the goal of this thread was to do micropropagation using OTC materials?
Of course it is going to need hormones... unless you are simply trying to grow a plant in agar... growing a plant in agar is easy - not so with getting the right ratio of certain types of plant hormones to form undifferentiated plant cells, or to cause them to grow shoots and roots...


What is the goal of this thread?
I was simply providing things that are useful for micropropagation in general.

What sort of data are you wanting too see, if any?


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OfflineDjcorbetto
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Re: Micro propagation at home. [Re: Burbles]
    #14090846 - 03/09/11 04:20 AM (12 years, 10 months ago)

I think he may be trying to say that all this extra information is confusing the thread. I read this to see how his tissue culture is going because im interested in tissue culturing cannabis plants, so to me all the extra bits are rather confusing, every time anyone asks about tissue culturing i send them to get the plants from test tubes books as thats the best thing you can have. I wanted to learn from this thread the method that doc t is using to see if it will work and efficiently produce some good cultures and the recipe that he's using because Ive heard of some weird things being used in the agar but never miracle grow. Keep up the good work Doc T, I've got my baby good jars coming in the next couple of days so i can start looking at the right ingredients that i need. Good luck


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Invisiblelucas_southoz
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Re: Micro propagation at home. [Re: Djcorbetto]
    #14107022 - 03/12/11 01:12 AM (12 years, 10 months ago)

Much happening with em doc?


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Offlinehumawebdesign
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Re: Micro propagation at home. [Re: lucas_southoz]
    #14107334 - 03/12/11 03:22 AM (12 years, 10 months ago)

i got some nice idea from all you peoples post hope my iea will work if everything goes fine then i will post it here.


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OfflineDjcorbetto
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Re: Micro propagation at home. [Re: humawebdesign]
    #14107381 - 03/12/11 03:51 AM (12 years, 10 months ago)

yo should tell us while you are doing it, that way people can give you advice on what to do as you are doing so you have less chance to fail and you might produce better results. of course set up a new thread for it


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Invisiblecacharstar
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Re: Micro propagation at home. [Re: humawebdesign]
    #14107487 - 03/12/11 04:50 AM (12 years, 10 months ago)

Quote:

humawebdesign said:
i got some nice idea from all you peoples post hope my iea will work if everything goes fine then i will post it here.





Good luck.  Post a link back here if you start a thread...


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InvisibleDoc_T
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Re: Micro propagation at home. [Re: cacharstar]
    #14135654 - 03/17/11 08:11 AM (12 years, 10 months ago)

First attempt: not successful.
The one bit of leaf seems to be doing ok, maybe even growing. But it's not exactly thriving.
Meanwhile, some sort of contamination has happened, most likely bacterial.



Next attempt: cacharstar supplied some cactus samples I'll be trying.
Growth medium this time is half-strength MG water, plus sucrose.
Updates in a couple weeks I hope.


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OfflineDjcorbetto
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Re: Micro propagation at home. [Re: Doc_T]
    #14137983 - 03/17/11 05:20 PM (12 years, 10 months ago)

I took delivery of baby food jars yesterday so im procuring my supplies and then i will start cannabis tissue culture, i will let you know how it goes.


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InvisibleDoc_T
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Re: Micro propagation at home. [Re: Djcorbetto]
    #14137996 - 03/17/11 05:21 PM (12 years, 10 months ago)

Woot!


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You make it all possible. Doesn't it feel good?


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OfflineDjcorbetto
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Re: Micro propagation at home. [Re: Doc_T]
    #14142953 - 03/18/11 12:20 PM (12 years, 10 months ago)

Quote:

Doc_T said:
Woot!




My plan has been to tissue culture, rare strains of the cannabis plant, i was waiting for my friend to send me the baby food jars that he had collected for me. just need to buy the rest of the ingredients now.


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Offlinecaricapapaya
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Re: Micro propagation at home. [Re: Djcorbetto]
    #14143236 - 03/18/11 01:34 PM (12 years, 10 months ago)

yes, the contams are bacterial. I think you'd have better luck with microprop in the strict sense of multiplying by axillary buds.

I dont think you'd even need hormones.

let us know


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