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OfflineLurkingWizards
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grain 2 grain: how long/far do YOU stretch your spawn?
    #14012842 - 02/23/11 10:34 AM (12 years, 11 months ago)

I'd like to gather some data from the community, possibly turn this topic into a pole...unless it's already been done, lol

Just some ideas on 'grain to grain'(g2g) first...

We could have a question on the poll concerning the different ratios used to transfer spawn... (for example: i use 1 pint to 7-8 quarts... so ~ 1:15 )
A. I do not preform grain to grain transfers
B. I use ratios in the range of 1:4-1:8
C. I use ratios in the range of 1:8-1:15
D. I use ratios in the range of 1:15 or more

something like that for starters...


my goal would be to expand the 'basic' concept/idea... getting the most out of your spawn.

Whether you start with MS, LC, isolates is not important for this particular exercise... maybe in the future, we can focus on the differences between the starting points-"master-jars"-using this term loosely to define the first colonized jar used to begin the transfer of spawn exponentially...

I guess a 'master-jar' (when transferred) would 'create' the equivalent of 'first-generation-spawn'... either from MS or an LC inoculation or agar piece/isolate, whichever is the original jar used to start the grain transfer = 'master-jar'.

'First-generation.' spawned to 'second-gen.'  Then, the 'second-gen.'(loose term) spawn could then be transferred again creating 'third-gen.'(loose term) and so on...(feel free to correct my term usage, just trying to convey the message here)


So, next question... how many times do you 'perpetuate' your spawn?...
Master-jar~->1st(Gen.)~->2nd(Gen.)~->3rd(Gen.)?.... that sort of idea, for example: I will keep transferring for as many 'generations' as possible, usually lasting about 2-3 months before the spawn is noticeably slower at colonizing to 100%.

and if I G2g every 10-14 days, the '7th-generation' is when I start to notice the decrease in 'mycelial-vigor'...

using this concept I only start over from 'new-master-jars' every 10 weeks or so.  Every time I make tubs, I keep one colonized jar for transferring to 'perpetuate' the spawn... two weeks later, repeat... then prepare 'new-masters' every 10 weeks...


I guess all I'm really trying to ask is if other people run into these types of questions or ideas, or if they expand their spawn in a similar way using 'mycelial-momentum', lol, something like that... I thought a pole would be interesting, but it would be difficult to construct one that isn't too confusing. :picard:

I'll get back to this later because my brains hurts now, but post your thoughts and experiences with G2G transfers and 'getting to most from your spawn'...

to be continued...


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InvisibleDoc_T
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Re: grain 2 grain: how long/far do YOU stretch your spawn? [Re: LurkingWizards]
    #14013154 - 02/23/11 12:03 PM (12 years, 11 months ago)

I use 1/4 to 1/2 mL of LC to start pint jars. If they are clean, I do g2g into 6-7 quart jars.

Is that what you were asking?


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OfflineLurkingWizards
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Re: grain 2 grain: how long/far do YOU stretch your spawn? [Re: Doc_T]
    #14013234 - 02/23/11 12:23 PM (12 years, 11 months ago)

Quote:

Doc_T said:
I use 1/4 to 1/2 mL of LC to start pint jars. If they are clean, I do g2g into 6-7 quart jars.

Is that what you were asking?





yes, but I was also wondering, if those 6-7 quart jars are only made into tubs or if the spawn is then expanded further?

...asking if you 'perpetuate' the spawn further, and if so, how many g2g transfers?

because I use the 6 quarts to make tubs then g2g that last jar to 6-7 more quart jars... then repeat for 'generations' before returning to the starting point(LC).  I start from LC every 10 weeks or so, but keep that spawn generating from g2g'n every 2 weeks...until I start noticing that the jars are colonizing slower(3 weeks) to get 100%...

does that help?  I make it sound more confusing sometimes than what I mean, lol.


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Re: grain 2 grain: how long/far do YOU stretch your spawn? [Re: LurkingWizards]
    #14013253 - 02/23/11 12:26 PM (12 years, 11 months ago)

Oh. No, I don't cascade it like that, but you certainly can for a few times.
No reason you can't end up with several hundred jars from one master.


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OfflineLurkingWizards
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Re: grain 2 grain: how long/far do YOU stretch your spawn? [Re: Doc_T]
    #14013410 - 02/23/11 12:59 PM (12 years, 11 months ago)

I don't really expand it exponentially though... like into 100's of jars... but, maybe one day if I obtain the time and space :smile2:

I meant that I just keep a constant 10-14 day cycle of making tubs from jars, but I always keep one jar back for g2g into another set of jars for re-colonization... repeating this cycle 6-8 times over 2-3 months before returning to the original LC in 'stasis'.

Most of the time I only use the LC once or twice before I start a new project or re-isolate from a fruit-body and make a 'newer-LC-isolate' or whatever's happening atm...

I was mainly wanting to get some input from this community about this same concept...

because I can rely on the consistent amount of time it takes to colonize my jars from g2g transfers better than LC... when I start a 'master-jar', it can take up to a month to fully colonize(usually 18-24 days for me, but very inconsistent). Where as the g2g is always 10-14 days for up to 6+ generations.

I like being able to rely on the g2g method for a few generations because all the jars colonize at the same rate... this may just be something that happens to me. :shrug:

Don't get me wrong, there's almost nothing sweeter than a fresh master-jar from isolate that colonizes wicked-fast... but why not perpetuate that spawn for as long as possible using that 'mycelial-momentum' to our advantage?  That LC will last longer too, right?

thanks for the response, I appreciate the feedback. :murray:


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Re: grain 2 grain: how long/far do YOU stretch your spawn? [Re: LurkingWizards]
    #14013431 - 02/23/11 01:03 PM (12 years, 11 months ago)

So you're fine for 6-8 'generations', then you start to see decline?
Then that's your answer. It's probably partly genetic, like human longevity.


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Re: grain 2 grain: how long/far do YOU stretch your spawn? [Re: LurkingWizards]
    #21011165 - 12/22/14 07:13 AM (9 years, 1 month ago)

My biggest question i been asking is how do you make a new "master jar"? Do you have to do it with one of the mushrooms like take that cut a stem off let that colonize than make a jar from that, would that be back to generation 0 or would that be the current grow generation plus one? Thanks for the help -kushroom


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