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OfflineJabensis
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Registered: 02/14/07
Posts: 541
Loc: Copperhead Road
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Long live the strain
    #14003693 - 02/21/11 08:13 PM (13 years, 12 days ago)

So about 5 months ago I was teaching my friend the art of mycology.  I showed him how to make LC and then when it had grown out, I had him inoculate 3 jars of 50:50 rye:wheat spawn.  Immediately one of the spawn quarts sped ahead of its brothers and collonized the jar in a matter of days.

Being a 5 year practitioner, I knew this was something special, especially when its brothers would cover the same ground in triple the time.  I decided that this "A" culture was just too good to fruit immediately and potentially lose the genes.  Since then Ive done a few grain transfers of the A culture, trying different mixes of grains along the way. But never actually fruited it(I am currently waiting on a straw tub of this culture to pin and another bin around 80% coll).

Parallelling the straw tub, I also have 9 gallons of WBS collinizing.  Figured WBS was the only substrate I havent done, so it was about time.
WBS PREP
-soak 16 lbs of WBS for 18 hours with 6 cups gypsum
-boil mash for 10-30 minutes
load into jars 2/3 full with a quarter cup dry verm to soak up excess water
-Save the WBS overnight soak "broth" for use in culture plates later
-PC for 55 minutes

The stack of jars you see were inoculated with that old 50:50 mix 3 days ago.  You decide for yourself if you think its a fast collinizer.

Also, a pic of the incubator.

if your curious about the straw bin, ill post pics in about a week when the primordia develop.



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J

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Offlinei GrOw StUFF
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Re: Long live the strain [Re: Jabensis]
    #14003720 - 02/21/11 08:19 PM (13 years, 12 days ago)

Is it a strain or just multispore?.....In order to get a strain you must isolate on perti dishes


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The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.

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OfflineJabensis
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Re: Long live the strain [Re: i GrOw StUFF]
    #14007024 - 02/22/11 12:01 PM (13 years, 11 days ago)

I can not say for certain that this is a single culture because the first inoculation was done via Liquid Culture(LC).  im certain there are only a few different types of genes in the culture because all the subsequent transfers exhibit the same exact behavior;  tenacious growth and speedy recovery.

I suppose I will have to do some agar work before boasting single culture.


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J

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Offlinek00laid
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Re: Long live the strain [Re: Jabensis]
    #14007086 - 02/22/11 12:11 PM (13 years, 11 days ago)

as a 5 year practitioner i would have thought you'd have heard the ol' saying "sometimes the fastest colonizers are the poorest fruiters."


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AMU - AMU Q & A - MyVideo Teks!

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Offline3n1gm4
3N!9M4T!C
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Re: Long live the strain [Re: k00laid]
    #14007122 - 02/22/11 12:17 PM (13 years, 11 days ago)

Where did you get the LC, are you sure its not "A" strain of mold? Have you actually seen the fruits, smelled the colonized jar anything?


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http://www.shroomery.org/6257/Magic-Mushroom-Dosage-CalculatorLOL when you zoom in to try to read my sig pics you will get lost in the crystal forrest of ghanni!

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Offlinear1es
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Re: Long live the strain [Re: 3n1gm4]
    #14008080 - 02/22/11 03:07 PM (13 years, 11 days ago)

u sure its not contam?

did you give any of the jars a whiff ?


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":mushroom2:here is nothing lost or wasted in this life.” - Bhagavad Gita

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OfflineJabensis
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Re: Long live the strain [Re: ar1es]
    #14012793 - 02/23/11 10:23 AM (13 years, 10 days ago)

I give all my jars a good whiff to confirm the mycelium smell.

Also, I had never heard of the old saying "fast coll, poor fruiter"  I hope this is nothte case for my current culture.  good to know though so thanks for calling it to my attention.


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J

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