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OfflineHeadrom
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Registered: 02/09/11
Posts: 9
Last seen: 11 years, 9 months
contams after a month
    #14012041 - 02/23/11 05:38 AM (12 years, 11 months ago)

so this is my first post here

i just started this hobby, so please be gentle with me :wink:

i did 6 pint jars with pollyfil and rye, cloned some fruits from the box( after a short soak in peroxide) and all of them where colonising great for about a month, then green started showing in the little shallow thing on the rye, they got tossed

did the same thing again 3 rye and 3 popcorn, and now after a month the rye started to go green again in the same spot again, corn is still fine , but abit slower colonising

so my questions are, does the contam come from the rye itself or from me not pc'ing them enough ? i tried to follow sterile procedure in a glove box spryed down with disinfectant

headrom


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InvisibleSillicybin
Registered: 02/14/05
Posts: 2,134
Loc: Flag
Re: contams after a month [Re: Headrom]
    #14012074 - 02/23/11 06:06 AM (12 years, 11 months ago)

Do another run.  This time, PC a jar that you don't do anything with except watch.

If all the other jars contaminate, it's your inoculum.  If that jar contaminates, it's because you're not getting full sterilization.

As a side note, it's almost certain that the cloning/transfer is the problem.  Mold is easily killed by heat.  If it were a bacterial infection, it would be more likely that sterilization was the problem.


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OfflineHeadrom
Stranger
Registered: 02/09/11
Posts: 9
Last seen: 11 years, 9 months
Re: contams after a month [Re: Sillicybin]
    #14018255 - 02/24/11 04:26 AM (12 years, 11 months ago)

im pretty sure your right about the inoc, next run will include a bought spore syringe and a test jar , no more tossing peroxided mushroom chunks in jars :tongue: and thanks for the help !


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InvisibleSillicybin
Registered: 02/14/05
Posts: 2,134
Loc: Flag
Re: contams after a month [Re: Headrom]
    #14018505 - 02/24/11 06:43 AM (12 years, 11 months ago)

You're quite welcome.  Best of luck on future grows. :thumbup:


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