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Offlinedmonkey1
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Dmonkey1's Bulk Block Growlog and Tek+ Pictorial
    #13875187 - 01/30/11 05:29 AM (13 years, 1 month ago)

Hey guys, I'm going to be documenting a different method published by Spongiform (https://www.shroomery.org/forums/showflat.php/Number/12826882#12826882).  This will be my first try at this method but using my prior knowledge to create my own take on spongi's idea.

I will be growing my strain which is an isolation between B+ and Texas.  This is my first tek-like contribution to the community and before I start I would like to thank everyone for the help along the way.  Even while I was still creeping around without an account
:monkeydance:


Materials:
Large spawn bags with filter patch (In this tek I used both .2 and 5 micron filters)
medium grade vermiculite
stabilized rice bran
bob red's 10 grain cereal

gypsum
spent coffee
alcohol wipes
sterile gauze
alcohol lamp
impulse sealer
mixing bucket
measuring cups
heavy duty medical tape

micropore tape

Step 1: Making the substrate

Mix your dry ingredients first.
  3 quarts of vermiculite and 1 quart of rice bran and 2 cups of different grains (I used BRF and Bob's red mills 10 grain)  in your bucket
 
Mix your gypsum and coffee into water
  1 quart of water + 2-3 tables spoons of coffee + 1/2 cup gypsum
 
 
  Add water and mix very well
 
 
  After a few minutes of stirring pour water onto dry mix and combine very well
 

Finished substrate:


Part 2: Bags and sterilization:
This part is very straight-forward.  Load your bags and pressure cook

The substrate recipe above is for one large filter bag.  Fill 'er up


for 5 micron bags, I seal them before sterilization

However for .2 micron bags you will have to prepare them for sealing directly after the sterilization process.  To best prepare them...

align the edges and tape with medical tape

Flip upside-down and push as much air out of the bag (too much air will make the bag explode)

Loosely roll the flaps (enough to let air out during the PC process) and tape

Load your pressure cooker (<3 to all the presto haters)
Pressure cook for 2 hours (120 minutes) at 15 psi

After the PC process. There is no need to remove the taped fap, seal directly underneath this will guarantee a sterile seal without a flowhood/Glove box

Part 3: inoculation
I created an Eberbach syringe of my strain (but LC and other liquid media can be used)

Open your sterile gauze and wipe the front of the bag with alcohol (70%)

Then place a strip of heavy duty medical tape over the sanitized area and sanitize the tape

Flame your needle and insert into the tape.  Use 30-40 mL/cc per bag depending on media type. treat it like a spawn bag, amount added is dependent on the moisture content of your substrate before entering the PC.  I sprayed in on the top, no penetration of substrate

Seal the hole with micropore tape (or another piece of heavy duty medical tape)



Thanks for looking.  I will post updates as it progresses

Quote:

dmonkey1 said:
update.

growth has started!  its been a day :smile:







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Edited by dmonkey1 (01/30/11 09:08 AM)

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Offlinedmonkey1
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dmonkey1]
    #13875189 - 01/30/11 05:30 AM (13 years, 1 month ago)

[[reserved for future updates]]


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Invisiblehiggledy-piggledy

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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dmonkey1]
    #13875228 - 01/30/11 05:51 AM (13 years, 1 month ago)

first of all, nice approach.

Quote:

dmonkey1 said:
Finished substrate:




this looks a bit too wet imo; almost muddy.

Quote:

dmonkey1 said:
Use 30-40 mL/cc per bag depending on media type.



that is really much. i think youre talking about 3-4 ml per bag, dont you?

im curious how this turns out.

:goodluck:

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Offlinedmonkey1
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: higgledy-piggledy]
    #13875235 - 01/30/11 05:55 AM (13 years, 1 month ago)

the water ratio actually puts it on the dry side.  it may look muddy due to the lighting and the huge clump is from the man-handling :smile:

maybe this is better pic?



30-40ml is correct since the substrate is on the dry-side.


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Edited by dmonkey1 (01/30/11 05:57 AM)

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InvisibleFractalXplora
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dmonkey1]
    #13875379 - 01/30/11 07:17 AM (13 years, 1 month ago)

Quote:

30-40ml is correct since the substrate is on the dry-side.




agreed theres no way that bags gonna colonize with 3-4ml solution. :nono:

good luck Dmonkey.

:popcorn:


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Offlinedmonkey1
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: FractalXplora]
    #13875591 - 01/30/11 09:08 AM (13 years, 1 month ago)

update.

growth has started!  its been a day :smile:



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Offlinegornyhuy
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dmonkey1]
    #13875617 - 01/30/11 09:20 AM (13 years, 1 month ago)

Very nice. I was just reading about spongiform's technique and bags and wondering if I should head in that direction. 

I have clean isolates on slants and agar, but I'm not sure what the best most contam-free method for getting myc into the syringe.  Could you explain how you prepared your Eberbach syringe?

Thanks!  :1up:


--------------------
:mushroom2:Bulk Grow Substrate Calculator Spreadsheet - Download :heartpump: Thread:mushroom2:
*Downloaded over 3,500 times!* Also try the DMT Changa Mix Calculator

Stonesun's Amazing Sclerotia/Stones Guide:mushroom2:Vasodilator List:mushroom2:Niacinamide - reduce trip anxiety


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InvisibleFractalXplora
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: gornyhuy]
    #13875863 - 01/30/11 10:46 AM (13 years, 1 month ago)

Im gonna have to have  another crack at the spongibag tek, my 1st one failed on its green ass!

I like the sound of hitting 'em up with a quart of colonized spawn rather than the LC, seems like a safer bet.

Its a cool way to go/grow.


--------------------

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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: FractalXplora]
    #13875973 - 01/30/11 11:18 AM (13 years, 1 month ago)

These blocks are awesome, the prep time/colonization times cant be beat IMO, since the bag can be rolled and a lot of LC can be used depending on the mix.  They can be dunked/rolled/fruited in a FC or GH and they can also be broken and spawned to bulk.
I love not messing with jars as much, saving them for LC.

IME so far they have been about as fast to colonize as grain jars but in 4 weeks I usually have a consolidated block.  In 3 weeks can be ready to spawn to bulk.  I haven't spawned one to bulk yet but I have a couple in the works and they are colonizing very very quickly.

I've been working with PE Blocks and just birthed one into an SGFC.

Thanks dmonkey1 for putting this together i love to see how various grain combos and "strains" work on these blocks.



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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: gornyhuy]
    #13878972 - 01/30/11 08:38 PM (13 years, 1 month ago)

Quote:

gornyhuy said:
Very nice. I was just reading about spongiform's technique and bags and wondering if I should head in that direction. 

I have clean isolates on slants and agar, but I'm not sure what the best most contam-free method for getting myc into the syringe.  Could you explain how you prepared your Eberbach syringe?

Thanks!  :1up:




I start from a spore print and start on MEA. I will make about 20 (3-part) dishes which creates about 60 inoculations. I take the top %10 with very rizomorphic and fast colonization qualities.  I then isolate each to a 15x100 MEA dish.  I let those grow out and proceed to do it again or make an eberbach LC

To make the eberbach LC, I wait until the dish is 80% colonized (no touching the edges).  I then sterilize 200-500mL of water for 25 minutes at 15 psi.  I then cut the colonized agar into the blender mechanism (all done under the hood).  Blend in 3 1/2 second bursts and then place the blend into a sterilized  500-1000mL Erlenmeyer flask with a sterilized stirbar and let the blend mix without heat (Warning, most stir-plates will produce heat when left on for more than a few hours).  I place half of an empty petridish to separate from heat.  Let stir for 24-48 hours at a very very slow speed.  This will make them want to colonized really badly and make it so that once they start combining they will explode with colonization speed.  Check out my update :smile:  It was taken less than 24 hours after inoculation.

I guess Ill write a pictorial when I get a chance


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Offlinegornyhuy
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dmonkey1]
    #13881398 - 01/31/11 11:49 AM (13 years, 1 month ago)

Quote:

dmonkey1 said:
Quote:

gornyhuy said:
Very nice. I was just reading about spongiform's technique and bags and wondering if I should head in that direction. 

I have clean isolates on slants and agar, but I'm not sure what the best most contam-free method for getting myc into the syringe.  Could you explain how you prepared your Eberbach syringe?

Thanks!  :1up:




I start from a spore print and start on MEA. I will make about 20 (3-part) dishes which creates about 60 inoculations. I take the top %10 with very rizomorphic and fast colonization qualities.  I then isolate each to a 15x100 MEA dish.  I let those grow out and proceed to do it again or make an eberbach LC

To make the eberbach LC, I wait until the dish is 80% colonized (no touching the edges).  I then sterilize 200-500mL of water for 25 minutes at 15 psi.  I then cut the colonized agar into the blender mechanism (all done under the hood).  Blend in 3 1/2 second bursts and then place the blend into a sterilized  500-1000mL Erlenmeyer flask with a sterilized stirbar and let the blend mix without heat (Warning, most stir-plates will produce heat when left on for more than a few hours).  I place half of an empty petridish to separate from heat.  Let stir for 24-48 hours at a very very slow speed.  This will make them want to colonized really badly and make it so that once they start combining they will explode with colonization speed.  Check out my update :smile:  It was taken less than 24 hours after inoculation.

I guess Ill write a pictorial when I get a chance




Thanks!  I'll have to try a ghetto version of this in a glove box with my home built stir plate.


--------------------
:mushroom2:Bulk Grow Substrate Calculator Spreadsheet - Download :heartpump: Thread:mushroom2:
*Downloaded over 3,500 times!* Also try the DMT Changa Mix Calculator

Stonesun's Amazing Sclerotia/Stones Guide:mushroom2:Vasodilator List:mushroom2:Niacinamide - reduce trip anxiety


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Offlinedmonkey1
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: gornyhuy]
    #13884210 - 01/31/11 07:57 PM (13 years, 1 month ago)

:dancer:


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InvisibleJavadog
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dmonkey1]
    #13884334 - 01/31/11 08:17 PM (13 years, 1 month ago)

Good work DM!

I hope that the experiments works out.

I liked the tricks-with-tape on the bag before PCing it.

Take care,

JD


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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: Javadog]
    #14006966 - 02/22/11 11:50 AM (13 years, 11 days ago)

Any updates on these blocks dmonkey?


--------------------

.
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: freeskierpj]
    #14008187 - 02/22/11 03:27 PM (13 years, 11 days ago)

Good Luck!  Will be waiting for updates :smile:

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Offlinedmonkey1
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dr0409]
    #14010066 - 02/22/11 09:15 PM (13 years, 10 days ago)

I had to move everything out of my lab due to a scare/scam from a member.  So they're in a cold place right now, still recovering from the cold shock (tons of condensation).  I'm gonna leave them there until they fully colonize, if they dont contam first.


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Edited by dmonkey1 (02/22/11 09:17 PM)

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Offlinefreeskierpj
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dmonkey1]
    #14010193 - 02/22/11 09:36 PM (13 years, 10 days ago)

Quote:

dmonkey1 said:
I had to move everything out of my lab due to a scare/scam from a member.  So they're in a cold place right now, still recovering from the cold shock (tons of condensation).  I'm gonna leave them there until they fully colonize, if they dont contam first.




Ah yea I forgot about you moving everything. It's a shame you lost the money you did and may have lost these bags as well if they do contam just cause some jackass wanted to scam the shroomery...


--------------------

.
-ProfessorPinHead
:amanita2::pinkshroom::supershroom::pinkshroom::amanita2:
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: freeskierpj]
    #14010212 - 02/22/11 09:38 PM (13 years, 10 days ago)

Karma is a bitch and that dude has a LOT of bad Karma.

I know it does not help in the moment, but know that the guy will at
some point or another receive a cosmic butt fuck. Here is to hoping!

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Offlinefreeskierpj
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: EvilMushroom666]
    #14010593 - 02/22/11 10:39 PM (13 years, 10 days ago)

Quote:

EvilMushroom666 said:
Karma is a bitch and that dude has a LOT of bad Karma.

I know it does not help in the moment, but know that the guy will at
some point or another receive a cosmic butt fuck. Here is to hoping!



:foshizzle: There's no way he doesn't have some bad shit coming his way...At the very least we know he is banned from the Marketplace and can't do it again here on the shroomery...He should be banned from the forums entirely but what can ya do...


--------------------

.
-ProfessorPinHead
:amanita2::pinkshroom::supershroom::pinkshroom::amanita2:
My Mycological Excursions
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Offlineiglweb
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: freeskierpj]
    #14010680 - 02/22/11 10:56 PM (13 years, 10 days ago)

Wow this is a sweet Tek...  I might add this to my weekly growing projects!

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