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dmonkey1
Monkey Say...




Registered: 06/29/10
Posts: 1,326
Loc: 39°50′39″N 75°42′...
Last seen: 11 years, 30 days
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Dmonkey1's Bulk Block Growlog and Tek+ Pictorial
#13875187 - 01/30/11 05:29 AM (13 years, 1 month ago) |
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Hey guys, I'm going to be documenting a different method published by Spongiform (https://www.shroomery.org/forums/showflat.php/Number/12826882#12826882). This will be my first try at this method but using my prior knowledge to create my own take on spongi's idea.
I will be growing my strain which is an isolation between B+ and Texas. This is my first tek-like contribution to the community and before I start I would like to thank everyone for the help along the way. Even while I was still creeping around without an account
Materials: Large spawn bags with filter patch (In this tek I used both .2 and 5 micron filters) medium grade vermiculite stabilized rice bran bob red's 10 grain cereal
 gypsum spent coffee alcohol wipes sterile gauze alcohol lamp impulse sealer mixing bucket measuring cups heavy duty medical tape
 micropore tape
Step 1: Making the substrate Mix your dry ingredients first. 3 quarts of vermiculite and 1 quart of rice bran and 2 cups of different grains (I used BRF and Bob's red mills 10 grain) in your bucket  Mix your gypsum and coffee into water 1 quart of water + 2-3 tables spoons of coffee + 1/2 cup gypsum   Add water and mix very well   After a few minutes of stirring pour water onto dry mix and combine very well 
Finished substrate:

Part 2: Bags and sterilization: This part is very straight-forward. Load your bags and pressure cook
The substrate recipe above is for one large filter bag. Fill 'er up

for 5 micron bags, I seal them before sterilization
However for .2 micron bags you will have to prepare them for sealing directly after the sterilization process. To best prepare them...
align the edges and tape with medical tape
 Flip upside-down and push as much air out of the bag (too much air will make the bag explode)
 Loosely roll the flaps (enough to let air out during the PC process) and tape
 Load your pressure cooker (<3 to all the presto haters) Pressure cook for 2 hours (120 minutes) at 15 psi
 After the PC process. There is no need to remove the taped fap, seal directly underneath this will guarantee a sterile seal without a flowhood/Glove box
Part 3: inoculation I created an Eberbach syringe of my strain (but LC and other liquid media can be used)
 Open your sterile gauze and wipe the front of the bag with alcohol (70%)
 Then place a strip of heavy duty medical tape over the sanitized area and sanitize the tape
 Flame your needle and insert into the tape. Use 30-40 mL/cc per bag depending on media type. treat it like a spawn bag, amount added is dependent on the moisture content of your substrate before entering the PC. I sprayed in on the top, no penetration of substrate
 Seal the hole with micropore tape (or another piece of heavy duty medical tape)

Thanks for looking. I will post updates as it progresses
Quote:
dmonkey1 said: update.
growth has started! its been a day 

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Edited by dmonkey1 (01/30/11 09:08 AM)
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dmonkey1
Monkey Say...




Registered: 06/29/10
Posts: 1,326
Loc: 39°50′39″N 75°42′...
Last seen: 11 years, 30 days
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dmonkey1]
#13875189 - 01/30/11 05:30 AM (13 years, 1 month ago) |
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[[reserved for future updates]]
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higgledy-piggledy

Registered: 08/24/10
Posts: 966
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dmonkey1]
#13875228 - 01/30/11 05:51 AM (13 years, 1 month ago) |
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first of all, nice approach.
Quote:
dmonkey1 said: Finished substrate:

this looks a bit too wet imo; almost muddy.
Quote:
dmonkey1 said: Use 30-40 mL/cc per bag depending on media type.
that is really much. i think youre talking about 3-4 ml per bag, dont you?
im curious how this turns out.
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dmonkey1
Monkey Say...




Registered: 06/29/10
Posts: 1,326
Loc: 39°50′39″N 75°42′...
Last seen: 11 years, 30 days
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: higgledy-piggledy]
#13875235 - 01/30/11 05:55 AM (13 years, 1 month ago) |
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the water ratio actually puts it on the dry side. it may look muddy due to the lighting and the huge clump is from the man-handling 
maybe this is better pic?

30-40ml is correct since the substrate is on the dry-side.
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Edited by dmonkey1 (01/30/11 05:57 AM)
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FractalXplora
Grainiack




Registered: 02/11/06
Posts: 2,494
Loc: UK
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dmonkey1]
#13875379 - 01/30/11 07:17 AM (13 years, 1 month ago) |
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Quote:
30-40ml is correct since the substrate is on the dry-side.
agreed theres no way that bags gonna colonize with 3-4ml solution. 
good luck Dmonkey.
--------------------

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dmonkey1
Monkey Say...




Registered: 06/29/10
Posts: 1,326
Loc: 39°50′39″N 75°42′...
Last seen: 11 years, 30 days
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: FractalXplora]
#13875591 - 01/30/11 09:08 AM (13 years, 1 month ago) |
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update.
growth has started! its been a day 
-------------------- Check out my Trade List!!
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gornyhuy
A Myth Intrepidly Met



Registered: 08/04/09
Posts: 2,933
Loc: USA
Last seen: 2 years, 3 months
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dmonkey1]
#13875617 - 01/30/11 09:20 AM (13 years, 1 month ago) |
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Very nice. I was just reading about spongiform's technique and bags and wondering if I should head in that direction.
I have clean isolates on slants and agar, but I'm not sure what the best most contam-free method for getting myc into the syringe. Could you explain how you prepared your Eberbach syringe?
Thanks!
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FractalXplora
Grainiack




Registered: 02/11/06
Posts: 2,494
Loc: UK
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: gornyhuy]
#13875863 - 01/30/11 10:46 AM (13 years, 1 month ago) |
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Im gonna have to have another crack at the spongibag tek, my 1st one failed on its green ass!
I like the sound of hitting 'em up with a quart of colonized spawn rather than the LC, seems like a safer bet.
Its a cool way to go/grow.
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N2DFlood
Fractalist



Registered: 11/10/10
Posts: 389
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: FractalXplora]
#13875973 - 01/30/11 11:18 AM (13 years, 1 month ago) |
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These blocks are awesome, the prep time/colonization times cant be beat IMO, since the bag can be rolled and a lot of LC can be used depending on the mix. They can be dunked/rolled/fruited in a FC or GH and they can also be broken and spawned to bulk. I love not messing with jars as much, saving them for LC.
IME so far they have been about as fast to colonize as grain jars but in 4 weeks I usually have a consolidated block. In 3 weeks can be ready to spawn to bulk. I haven't spawned one to bulk yet but I have a couple in the works and they are colonizing very very quickly.
I've been working with PE Blocks and just birthed one into an SGFC.
Thanks dmonkey1 for putting this together i love to see how various grain combos and "strains" work on these blocks.
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dmonkey1
Monkey Say...




Registered: 06/29/10
Posts: 1,326
Loc: 39°50′39″N 75°42′...
Last seen: 11 years, 30 days
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: gornyhuy]
#13878972 - 01/30/11 08:38 PM (13 years, 1 month ago) |
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Quote:
gornyhuy said: Very nice. I was just reading about spongiform's technique and bags and wondering if I should head in that direction.
I have clean isolates on slants and agar, but I'm not sure what the best most contam-free method for getting myc into the syringe. Could you explain how you prepared your Eberbach syringe?
Thanks! 
I start from a spore print and start on MEA. I will make about 20 (3-part) dishes which creates about 60 inoculations. I take the top %10 with very rizomorphic and fast colonization qualities. I then isolate each to a 15x100 MEA dish. I let those grow out and proceed to do it again or make an eberbach LC
To make the eberbach LC, I wait until the dish is 80% colonized (no touching the edges). I then sterilize 200-500mL of water for 25 minutes at 15 psi. I then cut the colonized agar into the blender mechanism (all done under the hood). Blend in 3 1/2 second bursts and then place the blend into a sterilized 500-1000mL Erlenmeyer flask with a sterilized stirbar and let the blend mix without heat (Warning, most stir-plates will produce heat when left on for more than a few hours). I place half of an empty petridish to separate from heat. Let stir for 24-48 hours at a very very slow speed. This will make them want to colonized really badly and make it so that once they start combining they will explode with colonization speed. Check out my update It was taken less than 24 hours after inoculation.
I guess Ill write a pictorial when I get a chance
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gornyhuy
A Myth Intrepidly Met



Registered: 08/04/09
Posts: 2,933
Loc: USA
Last seen: 2 years, 3 months
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dmonkey1]
#13881398 - 01/31/11 11:49 AM (13 years, 1 month ago) |
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Quote:
dmonkey1 said:
Quote:
gornyhuy said: Very nice. I was just reading about spongiform's technique and bags and wondering if I should head in that direction.
I have clean isolates on slants and agar, but I'm not sure what the best most contam-free method for getting myc into the syringe. Could you explain how you prepared your Eberbach syringe?
Thanks! 
I start from a spore print and start on MEA. I will make about 20 (3-part) dishes which creates about 60 inoculations. I take the top %10 with very rizomorphic and fast colonization qualities. I then isolate each to a 15x100 MEA dish. I let those grow out and proceed to do it again or make an eberbach LC
To make the eberbach LC, I wait until the dish is 80% colonized (no touching the edges). I then sterilize 200-500mL of water for 25 minutes at 15 psi. I then cut the colonized agar into the blender mechanism (all done under the hood). Blend in 3 1/2 second bursts and then place the blend into a sterilized 500-1000mL Erlenmeyer flask with a sterilized stirbar and let the blend mix without heat (Warning, most stir-plates will produce heat when left on for more than a few hours). I place half of an empty petridish to separate from heat. Let stir for 24-48 hours at a very very slow speed. This will make them want to colonized really badly and make it so that once they start combining they will explode with colonization speed. Check out my update It was taken less than 24 hours after inoculation.
I guess Ill write a pictorial when I get a chance
Thanks! I'll have to try a ghetto version of this in a glove box with my home built stir plate.
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dmonkey1
Monkey Say...




Registered: 06/29/10
Posts: 1,326
Loc: 39°50′39″N 75°42′...
Last seen: 11 years, 30 days
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: gornyhuy]
#13884210 - 01/31/11 07:57 PM (13 years, 1 month ago) |
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dmonkey1]
#13884334 - 01/31/11 08:17 PM (13 years, 1 month ago) |
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Good work DM!
I hope that the experiments works out.
I liked the tricks-with-tape on the bag before PCing it.
Take care,
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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freeskierpj
Just trying to learn some shit



Registered: 10/12/10
Posts: 1,671
Loc: Up, up and away.
Last seen: 2 years, 8 months
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: Javadog]
#14006966 - 02/22/11 11:50 AM (13 years, 11 days ago) |
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Any updates on these blocks dmonkey?
--------------------
. -ProfessorPinHead
    My Mycological Excursions Anything posted on these forums is strictly for fun and completely fictional.
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dr0409
Stranger



Registered: 08/21/09
Posts: 100
Loc: Alaska
Last seen: 9 years, 11 months
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: freeskierpj]
#14008187 - 02/22/11 03:27 PM (13 years, 11 days ago) |
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Good Luck! Will be waiting for updates
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dmonkey1
Monkey Say...




Registered: 06/29/10
Posts: 1,326
Loc: 39°50′39″N 75°42′...
Last seen: 11 years, 30 days
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dr0409]
#14010066 - 02/22/11 09:15 PM (13 years, 10 days ago) |
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I had to move everything out of my lab due to a scare/scam from a member. So they're in a cold place right now, still recovering from the cold shock (tons of condensation). I'm gonna leave them there until they fully colonize, if they dont contam first.
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Edited by dmonkey1 (02/22/11 09:17 PM)
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freeskierpj
Just trying to learn some shit



Registered: 10/12/10
Posts: 1,671
Loc: Up, up and away.
Last seen: 2 years, 8 months
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: dmonkey1]
#14010193 - 02/22/11 09:36 PM (13 years, 10 days ago) |
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Quote:
dmonkey1 said: I had to move everything out of my lab due to a scare/scam from a member. So they're in a cold place right now, still recovering from the cold shock (tons of condensation). I'm gonna leave them there until they fully colonize, if they dont contam first.
Ah yea I forgot about you moving everything. It's a shame you lost the money you did and may have lost these bags as well if they do contam just cause some jackass wanted to scam the shroomery...
--------------------
. -ProfessorPinHead
    My Mycological Excursions Anything posted on these forums is strictly for fun and completely fictional.
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: freeskierpj]
#14010212 - 02/22/11 09:38 PM (13 years, 10 days ago) |
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Karma is a bitch and that dude has a LOT of bad Karma.
I know it does not help in the moment, but know that the guy will at some point or another receive a cosmic butt fuck. Here is to hoping!
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freeskierpj
Just trying to learn some shit



Registered: 10/12/10
Posts: 1,671
Loc: Up, up and away.
Last seen: 2 years, 8 months
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: EvilMushroom666]
#14010593 - 02/22/11 10:39 PM (13 years, 10 days ago) |
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Quote:
EvilMushroom666 said: Karma is a bitch and that dude has a LOT of bad Karma.
I know it does not help in the moment, but know that the guy will at some point or another receive a cosmic butt fuck. Here is to hoping!
There's no way he doesn't have some bad shit coming his way...At the very least we know he is banned from the Marketplace and can't do it again here on the shroomery...He should be banned from the forums entirely but what can ya do...
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. -ProfessorPinHead
    My Mycological Excursions Anything posted on these forums is strictly for fun and completely fictional.
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iglweb
Stranger

Registered: 01/23/11
Posts: 16
Last seen: 11 years, 11 months
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Re: Dmonkey1's Bulk Block Growlog and Tek+ Pictorial [Re: freeskierpj]
#14010680 - 02/22/11 10:56 PM (13 years, 10 days ago) |
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Wow this is a sweet Tek... I might add this to my weekly growing projects!
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