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Mr. Mushrooms said:

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Alan Rockefeller said:

Measuring spores by hand introduces a lot of error.  Its better to use the Mr. Mushrooms method, where you hold a camera to the microscope reticle to photograph the spores and grid, then use a photo editor like The Gimp or Photoshop to count the number of pixels wide and long each spore is.  Then measure the grid, calculate the number of pixels per micrometer, and use math to turn the number of pixels into micrometers.  This eliminates lots of sources of error and gives you measurements that are exact to the nearest ten nanometers, more or less.  Probably less but that doesn't really matter, its cool to be able to say "this spore is 9.67 microns long".

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Hey, that was supposed to be our secret.    Seriously though, thanks for the credit.  I've used both methods but prefer mine for accuracy.

Also, after calibrating my way I merely hold a ruler up to the monitor and measure the spores, doing the math.  I can measure 30 spores in less than 5 minutes.  The standard way takes far longer.

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pepper said:
Do you have a link to your TEK where you use a camera and photo editor to measure spores? Thanks Mr. Mushrooms.

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