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InvisibleWilliam J. McGuire
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Registered: 01/28/11
Posts: 53
Germination? *DELETED*
    #13995578 - 02/20/11 01:02 PM (12 years, 11 months ago)

Post deleted by William J. McGuire

Reason for deletion: ..



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Offlinemmcakes
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Re: Germination? [Re: William J. McGuire]
    #13995716 - 02/20/11 01:38 PM (12 years, 11 months ago)

need more info.
type of jars
type of lids
grain preparation technique.
sterilization technique.
etc.


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OfflineTira
I'm a teapot


Registered: 11/20/10
Posts: 1,202
Loc: Turkey
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Re: Germination? [Re: mmcakes]
    #13995748 - 02/20/11 01:48 PM (12 years, 11 months ago)

tell us everything

Quote:

mmcakes said:
need more info.
type of jars
type of lids
grain preparation technique.
sterilization technique.





temperature
where do you keep them?
do they have filters? etc


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InvisibleWilliam J. McGuire
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Re: Germination? [Re: William J. McGuire]
    #13995936 - 02/20/11 02:28 PM (12 years, 11 months ago)

quart jars, rye berries, incubation temp 79 - 81 degrees. There in a tub in tub; tubs not translucent. Tyvek filters. Grains prepared by soak and then simmering for 30 minutes (so as to be on the dry side of wet). PC for 1 hour 15 psi. Let cool overnight; inoculated in glove box, 2cc - 2.5cc for each jar.


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Edited by William J. McGuire (02/20/11 02:30 PM)


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Invisiblevirus1824
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Re: Germination? [Re: William J. McGuire]
    #13995968 - 02/20/11 02:36 PM (12 years, 11 months ago)

I'd wait a bit longer. Did you shake after injecting the spores? if not. good!


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InvisibleWilliam J. McGuire
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Registered: 01/28/11
Posts: 53
Re: Germination? [Re: William J. McGuire]
    #13995969 - 02/20/11 02:36 PM (12 years, 11 months ago)

save this thread! This seems to be a common problem that is never addressed!


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InvisibleWilliam J. McGuire
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Re: Germination? [Re: virus1824]
    #13995976 - 02/20/11 02:37 PM (12 years, 11 months ago)

Oh I will, no question.


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Invisiblevirus1824
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Re: Germination? [Re: William J. McGuire]
    #13996003 - 02/20/11 02:42 PM (12 years, 11 months ago)

not that it matters right now but do you have a lid on your tub in tub setup?


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InvisibleWilliam J. McGuire
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Re: Germination? [Re: virus1824]
    #13996209 - 02/20/11 03:32 PM (12 years, 11 months ago)

Yes I have a lid on it.


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Invisiblevirus1824
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Registered: 09/25/05
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Re: Germination? [Re: William J. McGuire]
    #13996225 - 02/20/11 03:35 PM (12 years, 11 months ago)

don't. You're jars need gas exchange. You do not want a closed air environment.


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InvisibleWilliam J. McGuire
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Re: Germination? [Re: virus1824]
    #13996756 - 02/20/11 05:20 PM (12 years, 11 months ago)

But if there is no germination, what needs to be exchanging gas? Is that a trigger for germination?


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Offlinedr0409
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Re: Germination? [Re: William J. McGuire]
    #13996965 - 02/20/11 05:59 PM (12 years, 11 months ago)

IME rye jars can take up to 14 days some times longer to see growth from a spore syringe.  I wouldn't worry about it.  And scene you are incubating them (which is not needed) use a towel or blanket on top instead of the lid.  Hope it all works out for ya.


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OfflineTira
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Registered: 11/20/10
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Re: Germination? [Re: William J. McGuire]
    #13998381 - 02/20/11 09:50 PM (12 years, 11 months ago)

Quote:

William J. McGuire said:
But if there is no germination, what needs to be exchanging gas? Is that a trigger for germination?




yea its a trigger for germination. i had the same setup too. just open the lid of your tubs and fan with the lid 3-5 times a day WITHOUT OPENING THE LIDS OF THE JARS. my jars did good. you can check it out from the link of my first grow in my signature.


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


Extras: Filter Print Post Top
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