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OfflineTerry M
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Agar to PF transfer?
    #13990402 - 02/19/11 02:59 PM (12 years, 11 months ago)

I am getting started with agar, and currently have some plates from which I am trying to isolate a strain. I am following RR's DVD instructions. When I get to my many different culture strains to fruit out, I would like to use PF jars instead of grain jars. This seems a perfect use for half-pint cheap, fast-to-fruit PF cakes, vs. the same number of large and costly quart grain jars.

Surely I am not the only one who has thought of this. My question is, how do I do an agar to PF jar transfer? If I cut out a piece of colonized agar and open my sterile PF jar to drop it in, I have a top layer of pure verm to deal with.

What do people do? dump out the layer of verm, drop the agar piece onto the brown rice-covered verm, and recover with pure verm? Or maybe sterilize some PF jars without the top layer of verm, and add some sterile verm back after I have dropped in the agar piece? Either way, it seems like too much time for my jars to be uncovered. There's gotta be a good way to do this!


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InvisibleStains Blue
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Re: Agar to PF transfer? [Re: Terry M]
    #13990463 - 02/19/11 03:12 PM (12 years, 11 months ago)

I would think the easiest way to do this would be

Agar>LC>BRF Jar

Isolate on agar, then transfer to LC let that grow out, then use a syringe to transfer that to you PF Jars.


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Offlinedmonkey1
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Re: Agar to PF transfer? [Re: Stains Blue]
    #13990473 - 02/19/11 03:14 PM (12 years, 11 months ago)

eberbach LC is an easier LC if going from agar


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Re: Agar to PF transfer? [Re: dmonkey1]
    #13990478 - 02/19/11 03:15 PM (12 years, 11 months ago)

Yeah that too ^


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OfflineTerry M
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Re: Agar to PF transfer? [Re: Stains Blue]
    #13990597 - 02/19/11 03:38 PM (12 years, 11 months ago)

But then I'd have to make 20-30 LC jars. I have made a dozen quart-sized LC jars, and found that installing the self-healing injection ports and gas exchange filters is a fair amount of work.


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Edited by Terry M (02/19/11 03:40 PM)


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Re: Agar to PF transfer? [Re: Terry M]
    #13990640 - 02/19/11 03:46 PM (12 years, 11 months ago)

20-30 LC jars?  That will inoculate 100, 10 pound spawn bags.  You're doing PF pint jars.


You need to read more.


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OfflinePsilocyentist
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Re: Agar to PF transfer? [Re: Terry M]
    #13990803 - 02/19/11 04:13 PM (12 years, 11 months ago)

Quote:

Terry M said:
But then I'd have to make 20-30 LC jars. I have made a dozen quart-sized LC jars, and found that installing the self-healing injection ports and gas exchange filters is a fair amount of work.



:laugh2:


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Re: Agar to PF transfer? [Re: Psilocyentist]
    #13990810 - 02/19/11 04:14 PM (12 years, 11 months ago)

Quote:

Psilocyentist said:

:laugh2:




:awesomenod:


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OfflineTerry M
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Re: Agar to PF transfer? [Re: dmonkey1]
    #13990971 - 02/19/11 04:52 PM (12 years, 11 months ago)

Quote:

Psilocyentist said:
Quote:

Terry M said:
But then I'd have to make 20-30 LC jars. I have made a dozen quart-sized LC jars, and found that installing the self-healing injection ports and gas exchange filters is a fair amount of work.



:laugh2:



Quote:

dmonkey1 said:
20-30 LC jars?  That will inoculate 100, 10 pound spawn bags.  You're doing PF pint jars.


You need to read more.




Huh???
I am isolatng strains, as I said in my original post. This results in many different mycelia that need to be separately fruited in order to determine the best ones.


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OfflineRogerRabbitM
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Re: Agar to PF transfer? [Re: Terry M]
    #13990990 - 02/19/11 04:54 PM (12 years, 11 months ago)

Simply leave the verm filter off and cut tyvek sleeves or lab coats into filters.  Lift the lid and drop the wedge in.  Use a glovebox if you don't have a flowhood.
RR


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Re: Agar to PF transfer? [Re: Terry M]
    #13991002 - 02/19/11 04:57 PM (12 years, 11 months ago)

yep i would skip the LC


Edited by dmonkey1 (02/19/11 04:58 PM)


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OfflineTerry M
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Re: Agar to PF transfer? [Re: RogerRabbit]
    #13991005 - 02/19/11 04:57 PM (12 years, 11 months ago)

Thanks, RR!


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OfflineTerry M
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Re: Agar to PF transfer? [Re: Terry M]
    #13991414 - 02/19/11 06:22 PM (12 years, 11 months ago)

I just made up a batch of PF jars with Tyvek instead of the top layer of verm. Am PCing them now!
:thumbup:


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Invisibleandymc
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Re: Agar to PF transfer? [Re: Terry M]
    #13991457 - 02/19/11 06:28 PM (12 years, 11 months ago)

Quote:

Terry M said:
I just made up a batch of PF jars with Tyvek instead of the top layer of verm. Am PCing them now!
:thumbup:




If you were going to use a dry verm layer, then the LC would've been necessary, as you wouldn't have been able to get the agar wedge under there in any reasonable sort of way.

Personally, I like LCs.  Baby bottle tek FTW.


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OfflineTerry M
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Re: Agar to PF transfer? [Re: andymc]
    #13991602 - 02/19/11 06:51 PM (12 years, 11 months ago)

Quote:

andymc said:
If you were going to use a dry verm layer, then the LC would've been necessary, as you wouldn't have been able to get the agar wedge under there in any reasonable sort of way.

Personally, I like LCs.  Baby bottle tek FTW.



Yup. I use LCs whenever I possibly can. I've got 7 different mushroom LCs in the frig now.


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Offlinetotal
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Re: Agar to PF transfer? [Re: dmonkey1]
    #13991731 - 02/19/11 07:23 PM (12 years, 11 months ago)

Quote:

dmonkey1 said:
eberbach LC is an easier LC if going from agar



:awesomenod:

Home Made Eberbach


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OfflineTerry M
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Re: Agar to PF transfer? [Re: total]
    #14006115 - 02/22/11 07:04 AM (12 years, 11 months ago)

Quote:

total said:
Quote:

dmonkey1 said:
eberbach LC is an easier LC if going from agar



:awesomenod:

Home Made Eberbach



Got myself a Waring blender as soon as I saw the "Ghetto Eberbach!"
:thumbup:


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