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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
Posts: 1,970
Loc: Scoping Woodchips
Last seen: 11 years, 7 days
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First Grow B+ -- PF Tek (pics)
#13989686 - 02/19/11 12:04 PM (12 years, 11 months ago) |
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So i had some fully colonized jars and i went to my friends house and stayed there for two nights and by the time i got home i had invitro pins growing in my pf jars. 
Im going to birth these as soon as possible. My questions are how should i go about birthing cakes with in vitro pins? Do i still do the 24 hour dunk and rool in dry verm?
And i lost my 1/4inch drill bit and i only can find 3/8...will this be bad if i drill all my holes in my SGFC with 3/8 drill bit? I will look for the 1/4 if so.
And i have to fill up the dunking water from my sink which is in the dirtiest environment in my house for growing mushrooms. There is stacks of potatoes and oranges that are covered in green mold on the average right by the sink.
I sprayed the area of the sink with 1:10 solution of bleach/water but there is lots of surfaces and towels and such that are good places for mold spores to stick to. I dont want to rinse my cakes off in that environment.
And rinsing the perlite with water. Im gonna have to do that over my dirty ass sink too. Im probly just gonna spray tons of bleach/water and wipe down And hope for the best
Any tips/advice would be great
Edited by OregonChronic (02/20/11 12:21 AM)
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DungeonMaster



Registered: 12/17/10
Posts: 436
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Re: First Pins Pinning In-Vitro! (pics) [Re: OregonChronic]
#13989723 - 02/19/11 12:11 PM (12 years, 11 months ago) |
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Go ahead and do the dunk & roll. It wouldn't damage the pins. In my experience, cleanliness isn't so important during the fruiting stage. Good luck!
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Boozie
I like beer.



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Re: First Pins Pinning In-Vitro! (pics) [Re: OregonChronic]
#13989734 - 02/19/11 12:12 PM (12 years, 11 months ago) |
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Yup, dunk and roll as usual, pins and all. They'll be fine.
My kitchen can get pretty dirty too, but I still do all my dunks/soaks right at the kitchen sink, with regular ol' tap water (I do clean it up a bit if there happens to be a larger than average mess though).
As for the drill bit, i'd think it would work okay. I've never used a SGFC before though, so you may want to wait for someone with a little more experience with 'em. 
edit: Reading comprehension ftw.
-------------------- "After silence, that which comes nearest to expressing the inexpressible is music." - Aldous Huxley
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Primal Call
Earth Mage



Registered: 09/05/10
Posts: 2,766
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Re: First Pins Pinning In-Vitro! (pics) [Re: DungeonMaster]
#13989748 - 02/19/11 12:15 PM (12 years, 11 months ago) |
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an 1/8" more probably wont make a huge difference. maybe just space them a tiny bit further from one another?
for the cleaning, get some air spray + the counter/sink wipedown and close any drafts off.
have fun! keep us updated!
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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
Posts: 1,970
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Re: First Pins Pinning In-Vitro! (pics) [Re: Primal Call]
#13989763 - 02/19/11 12:18 PM (12 years, 11 months ago) |
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Thats good to hear...it seems this pin is going to grow towards the perlite and raise the cake up from the bottom. I hope not.
We'll see how it goes 
Would it be better to use the 3/8 drill bit or the 1/8? Probly the 1/8 hah.
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Primal Call
Earth Mage



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Re: First Pins Pinning In-Vitro! (pics) [Re: OregonChronic]
#13989794 - 02/19/11 12:27 PM (12 years, 11 months ago) |
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with how important FAE is, I think the 3/8. just be sure to check its rH every day or two.
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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
Posts: 1,970
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Re: First Pins Pinning In-Vitro! (pics) [Re: Primal Call]
#13990185 - 02/19/11 02:03 PM (12 years, 11 months ago) |
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Alright i drilled holes on all 6 sides with the 3/8 
I will tap the cakes out of their jars today and dunk them to be rolled in verm tomorrow and birthed  
I will keep pics updated
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Edited by OregonChronic (02/20/11 03:02 AM)
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jacobensis
captain


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Re: First Pins Pinning In-Vitro! (pics) [Re: OregonChronic]
#13990238 - 02/19/11 02:15 PM (12 years, 11 months ago) |
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i used a 3/8`s when i was using a sgfc and everything went OK but i used a few less holes, as fare as your sink/kitchen goes, remember you obviously were clean enough to be successfull with your cakes so i wouldn't worry about that either. IMPORTANT: go to cigar shop and buy a cheep hydrometer! that stops ALL the GUESS work- you will be happy you did. remember to make spore prints.
-------------------- There are 2 types of people, mycophobes and mycophiles R.Wasson
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afrosheen
9Lives the cat



Registered: 03/06/10
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Re: First Pins Pinning In-Vitro! (pics) [Re: jacobensis]
#13990287 - 02/19/11 02:27 PM (12 years, 11 months ago) |
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Cheap hygrometer is here: http://www.bargainhumidors.com/bh/ProductInfo.aspx?productid=ACC-ANALOG
Tons of people have bought from them, they're adjustable and cheap.
3/8" holes may be too large. Remember kids, a 1/4" hole has 4x the area that a 1/8" hole does. If you went 3/8" every 2 inches on all six sides, you may want to tape up half of the holes. With a good hygrometer you can watch it and adjust as needed.
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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
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Last seen: 11 years, 7 days
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Re: First Pins Pinning In-Vitro! (pics) [Re: afrosheen]
#13990294 - 02/19/11 02:30 PM (12 years, 11 months ago) |
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Ya i made a good amount of holes but i didnt go all the way through with the drill bit so its a bit smaller than 3/8"
I'll see how the humidity goes
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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
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Re: First Pins Pinning In-Vitro! (pics) [Re: OregonChronic]
#13996222 - 02/20/11 03:34 PM (12 years, 11 months ago) |
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Alright its been 24 hours coming 3pm and im about take the cakes out and roll in dry verm..Im gonna sterilize the verm in the oven at 350F for how long? And should i have an aluminum foil cover in what i sterilize it in?
I dont want to roll the cakes in the verm in my kitchen because my kitchen is really dirty, and i have to soak perlite and fill a SGFC with it and also wash the SGFC in the same sink area which is dirty.
Im doin it in 30mins tho
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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
Posts: 1,970
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Re: First Pins Pinning In-Vitro! (pics) [Re: OregonChronic]
#13996559 - 02/20/11 04:36 PM (12 years, 11 months ago) |
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Should i clean my FC with bleach/water or just dish soap and water?
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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
Posts: 1,970
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Last seen: 11 years, 7 days
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Re: First Pins Pinning In-Vitro! (pics) [Re: OregonChronic]
#13996740 - 02/20/11 05:16 PM (12 years, 11 months ago) |
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Does it matter that they are still in the water I spaced and im sterilizing the dry verm in my oven at 350F right now and it will be cooled down hopefully soon. That'll be about 2 hours overdunked.
Should i clean the FC with bleach/water or dish soap/water?
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afrosheen
9Lives the cat



Registered: 03/06/10
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Re: First Pins Pinning In-Vitro! (pics) [Re: OregonChronic]
#13998010 - 02/20/11 08:56 PM (12 years, 11 months ago) |
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Soap and water is fine. Fully colonized cakes are supposed to be really tough and hard to contam. I never even baked the verm before.
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i GrOw StUFF
Stranger

Registered: 01/14/11
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Re: First Pins Pinning In-Vitro! (pics) [Re: afrosheen]
#13998060 - 02/20/11 09:04 PM (12 years, 11 months ago) |
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I would clean it with more than soap and water before I use it.....Maybe with alcohol or bleach water just to be safe (but I am an anal mofo when it comes to cleaning)....Always bake your dry verm too before you roll
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
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Re: First Pins Pinning In-Vitro! (pics) [Re: i GrOw StUFF]
#14000484 - 02/21/11 10:40 AM (12 years, 11 months ago) |
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It might be just me but my cakes seem kinda dry I dont know i'll just keep fanning/misting 3-4-5 times a day and will see how it goes.
I just birthed another 2 cakes lastnight at 10pm and i will roll those in dry verm later today. Should i only dunk for 12 hours this time?
Exactly how much should i be fanning and misting? How hard of a fanning and how hard of a misting?
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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
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Re: First Pins Pinning In-Vitro! (pics) [Re: OregonChronic]
#14001715 - 02/21/11 02:08 PM (12 years, 11 months ago) |
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Should i dunk these cakes for 12-16 hours this time or a full 24? The room i have the FC in is really really dirty and i mean dirty.
It was a room that someone kinda failed to grow in and there is still alot of soil like pounds of soil in tubs just sitting in the room with clothes and shit and dust and hair every where.
But thats the only place to fruit other than the closet im incubating in. But closets have shitty air flow.
Here are some pics of my FC and the sad environment it is in. Remember i had nothing to do with this room and i think it is sad.
My FC with the 2 cakes dunking on the left in that white container, and the mister on the right

And the sad environment i have my FC in

It really is uncleanable within 1 day so i said f it.
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: First Pins Pinning In-Vitro! (pics) [Re: OregonChronic]
#14001742 - 02/21/11 02:11 PM (12 years, 11 months ago) |
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"I remember my first bier"

hahaha
good stuff.. cant wait to see the mushies
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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
Posts: 1,970
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Last seen: 11 years, 7 days
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Re: First Pins Pinning In-Vitro! (pics) [Re: DJYoshaBYD]
#14001787 - 02/21/11 02:17 PM (12 years, 11 months ago) |
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Does it matter that my perlite is really uneven? I will be putting 2 more cakes in there and one of them is going on that corner with less perlite.
Will that effect its growth?
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: First Pins Pinning In-Vitro! (pics) [Re: OregonChronic]
#14001827 - 02/21/11 02:22 PM (12 years, 11 months ago) |
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you need a uniform layer of perlite, and it shouldnt be puddled up on the bottom.. i mean, a tad is ok, but not alot..
and yeah.. in order to get even distribution, it should be fairly level.. no perfect.. but it shouldnt have any major dips..
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