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OfflineForager
Mycophile

Registered: 02/15/11
Posts: 440
Last seen: 3 years, 18 days
Storage of Cultures and Spawn
    #13985388 - 02/18/11 03:42 PM (12 years, 11 months ago)

Hello fellow Mycophiles,
I am looking for advice on storing spawn.  I am interested in what form of spawn is most easy to store.  I have reading in GGMM that culture slants are the most effective means of storing cultures.  Is it possible, however, to simply store grain or wood spawn in a fridge?  What temp would be optimal for storing spawn?  I am worried that my spawn may be ready for my woodlovers patch before the local climate will actually allow for the inoculation of the bed, so I thought it might be useful to learn a little about storing spawn.  Thanks!


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InvisibleDoc_T
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Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Storage of Cultures and Spawn [Re: Forager]
    #13985401 - 02/18/11 03:45 PM (12 years, 11 months ago)

Fridge should be fine for a few months.


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OfflineForager
Mycophile

Registered: 02/15/11
Posts: 440
Last seen: 3 years, 18 days
Re: Storage of Cultures and Spawn [Re: Doc_T]
    #13985760 - 02/18/11 05:15 PM (12 years, 11 months ago)

Cool, I was under the impression that fridge storage would be alright as it would not stop the growth, merely slow it.  I have even read that cultures can be stored in liquid nitrogen.  How is this possible without significantly damaging the tissue of the mycelium? I would think such a rapid freeze would cause expansion of the water in the cells to rupture the cells.


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Always looking for interesting active prints (especially Gymnopilus spp. and sclerotia producers) and cacti and poppy seeds.


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InvisibleDZ
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Registered: 01/25/09
Posts: 381
Re: Storage of Cultures and Spawn [Re: Forager]
    #13987544 - 02/18/11 10:47 PM (12 years, 11 months ago)

Iono about liquid nitrogen but I've read some about mycelium water. From what I've read it is supposed to store longer than culture slants. The process I've read about involves putting mycelium in sterilized distilled water. The water has no nutrients so it's believed the mycelium goes dormant.


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InvisibleDZ
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Registered: 01/25/09
Posts: 381
Re: Storage of Cultures and Spawn [Re: DZ]
    #13987552 - 02/18/11 10:49 PM (12 years, 11 months ago)

Oh yeah and I've stored WBS in the fridge for somewhere around 3-4 months and had very little problem. Out of 20+ jars only 1 or 2 went bad but this was most likely due to using a single layer of post office tyvek as a contam barrier and/or inoculation procedure.


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InvisibleDoc_T
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Re: Storage of Cultures and Spawn [Re: DZ]
    #13987655 - 02/18/11 11:06 PM (12 years, 11 months ago)

I've stored grain jars on a shelf for a couple months.


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You make it all possible. Doesn't it feel good?


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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
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Re: Storage of Cultures and Spawn [Re: Forager]
    #13987700 - 02/18/11 11:12 PM (12 years, 11 months ago)

Quote:

Forager said:
Hello fellow Mycophiles,
I am looking for advice on storing spawn.  I am interested in what form of spawn is most easy to store.  I have reading in GGMM that culture slants are the most effective means of storing cultures.




For hobby and small commercial growers, master culture slants are the way to go.
RR


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