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InvisibleCyclohexylamine
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Issues with WBS
    #13983349 - 02/18/11 07:53 AM (12 years, 11 months ago)

Having alot of issues with WBS jars being sterile.
I have a PC, it has gotten to the point where I have been PCing for 90 minutes, and then another 60.
And they still get contamination at spots where the needle never touched (so it is definitely due to not being sterilized).

Any ideas?

Edit: The jars are not too wet.


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Edited by Cyclohexylamine (02/18/11 07:54 AM)


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InvisibleDoc_T
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Re: Issues with WBS [Re: Cyclohexylamine]
    #13983355 - 02/18/11 07:56 AM (12 years, 11 months ago)

How do you prep your grain?
You tested your inoculant on another medium like agar or a brf cake?
Are you shooting spores or LC?
You said the contam is in a different location- you don't shake grain jars after you shoot?

pics?


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InvisibleCyclohexylamine
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Re: Issues with WBS [Re: Doc_T]
    #13983365 - 02/18/11 07:59 AM (12 years, 11 months ago)

Sorry should have given more info -
I prep the grains basically following your method doc :P
Shooting spores this time (with the 90 + 60), however LC before.
And nope only shake them up before inoculation, and then 25% in. Not after inoculation, I heard its not as good.


--------------------
Yes this is tymo - I just changed my name

Have you ever had a dream that you were so sure was real? What if you were unable to awake from that dream? How would you know the difference between that dream world and the real world?


There is NOTHING better than feeling that warm dissociative fuzz creeping up your body from IM K
Something abut that anaesthetic rush... :inlove:

Qualitative Research Chemical Effects and Experiences
The Wonderful World of Methoxetamine
The 3-Meo-PCP Chapters, Part One


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Re: Issues with WBS [Re: Cyclohexylamine]
    #13983368 - 02/18/11 08:01 AM (12 years, 11 months ago)

Your LC is bad. :thumbdown:
Before you worry about changing your grain prep, get a known-clean culture on agar.

(I prep grain like RR. There's a member named Doc34 with a famous grain tek that I don't use. Just to clarify.)


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InvisibleCyclohexylamine
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Re: Issues with WBS [Re: Doc_T]
    #13983377 - 02/18/11 08:04 AM (12 years, 11 months ago)

Oh cool mixed up the two docs :P
And all the ones that I use syringes on have the same problem. As well I left a few jars without being opened after PCing and they got contaminated as well.


--------------------
Yes this is tymo - I just changed my name

Have you ever had a dream that you were so sure was real? What if you were unable to awake from that dream? How would you know the difference between that dream world and the real world?


There is NOTHING better than feeling that warm dissociative fuzz creeping up your body from IM K
Something abut that anaesthetic rush... :inlove:

Qualitative Research Chemical Effects and Experiences
The Wonderful World of Methoxetamine
The 3-Meo-PCP Chapters, Part One


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Re: Issues with WBS [Re: Cyclohexylamine]
    #13983399 - 02/18/11 08:15 AM (12 years, 11 months ago)

Hm. If all jars contam the same way, even unshot control jars, then yeah, it's the grain, or the grain prep, or your PC is faulty.
Try RR's method. He uses rye, wbs is smaller and you don't soak or simmer as long. (I do four hours of soak, then simmer until al dente).
Be sure to use wire mesh strainers with wbs, not plastic colanders. Steam it dry, don't rinse after the boil.

But you need to make some agar dishes or brf cakes anyway, to verify your syringes and to verify your PC.
I'm still betting the contam is in your syringe.


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