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barrier101
Newb Grower



Registered: 10/20/10
Posts: 181
Loc: Southwest
Last seen: 3 years, 7 months
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What am I doing wrong?
#13982084 - 02/17/11 11:09 PM (13 years, 15 days ago) |
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Okay. I'm on my second try with rye. I followed this tek to a T. It's been 10 days and I see nothing on most of the jars. 1 jar I spotted what I think might be trich. The funny thing is that it started growing right where I squirted in the spores. My first try I did the same tek and every single jar ended up getting what the community identified as trich. Did I get bad syringes? I even made filter lids with rtv silicone and tyvek to be more sterile. I don't know what the problem could be. Help!
Edit: Just checked the jars and about 6 of them have the same bluish growth. Why would I get trich 20 jars in a row?  Here are the pictures from before.
Edited by barrier101 (02/17/11 11:16 PM)
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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Re: What am I doing wrong? [Re: barrier101]
#13982219 - 02/17/11 11:28 PM (13 years, 15 days ago) |
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if you did a proper sterilization, then chances are bad syringe, or you were unsterile when innoculating.
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Traikun
Extermenieren!



Registered: 09/12/10
Posts: 722
Loc: BC, Canada
Last seen: 3 years, 23 days
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Re: What am I doing wrong? [Re: barrier101]
#13982237 - 02/17/11 11:31 PM (13 years, 15 days ago) |
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I have a syringe of chilean spores...I made an LC with it (which contaminated the jars I shot up), and I've so far made another 5 jars out of it, and they ALL contaminated. I did a couple other little tests with it, and so far everything has been 100% contam.
I've ruled out my sterile technique as the problem and attribute it to the spore syringe. If you have agar, shoot up some plates and see if you can't grow something other than trich, clean it up, and inoc with that. 
If you're sure of your sterile technique, it's likely your spores.
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New HooN
Showing the way



Registered: 01/15/11
Posts: 382
Loc: MI
Last seen: 11 years, 1 month
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Re: What am I doing wrong? [Re: Traikun]
#13982335 - 02/17/11 11:45 PM (13 years, 15 days ago) |
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bad syringes man sorry
--------------------
Ride it like u can fix it..... CR 500R
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barrier101
Newb Grower



Registered: 10/20/10
Posts: 181
Loc: Southwest
Last seen: 3 years, 7 months
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Re: What am I doing wrong? [Re: New HooN]
#13982626 - 02/18/11 12:40 AM (13 years, 15 days ago) |
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Flame sterilized the needle between jars in a glovebox sprayed with oust. What are the chances of 2 separate syringes ordered from ralphsters being contaminated?
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Larrythescaryrex
teardrop on the fire



Registered: 07/19/00
Posts: 11,004
Loc: further down the spiral
Last seen: 1 year, 2 months
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Re: What am I doing wrong? [Re: barrier101]
#13982672 - 02/18/11 12:51 AM (13 years, 15 days ago) |
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in the past ralphsters has been the best vendor in my opinion.
-------------------- RIP Acidic_Sloth Sunset_Mission said: "larry the scary rex verily scary when thoroughly vexed invoke the shadows and dust, cast a hex mercifully massacring memories masterfully relocate from Ur to 8th density and become a cosmic bully mulder and scully couldn't decipher his glyphs invoke the shadows and dust, smoke infernal spliffs" April 24th 2011
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Traikun
Extermenieren!



Registered: 09/12/10
Posts: 722
Loc: BC, Canada
Last seen: 3 years, 23 days
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Re: What am I doing wrong? [Re: barrier101]
#13983337 - 02/18/11 07:45 AM (13 years, 15 days ago) |
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Quote:
barrier101 said: Flame sterilized the needle between jars in a glovebox sprayed with oust. What are the chances of 2 separate syringes ordered from ralphsters being contaminated? 
Anything is possible. Since they get their prints from growers, if the print was contaminated - the syringe will be as well. My chilean syringe was received as "strain of the month" from ralphster Canada back in September.
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