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Shop: North Spore Cultivation Supplies, Injection Grain Bag   Mushroom-Hut Liquid Cultures

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Offlinemycocozm


Registered: 05/13/10
Posts: 181
Last seen: 3 years, 25 days
LC question
    #13981847 - 02/17/11 10:36 PM (12 years, 11 months ago)

1) How long should I let my liquid culture stir; how long for it to recover before using it for inoculation?


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Invisiblebiologys
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Re: LC question [Re: mycocozm]
    #13981853 - 02/17/11 10:37 PM (12 years, 11 months ago)

stir long enough to break up well enough to suck up in syringe..you can use it as soon as you get it in your syringe...no recovery time needed.


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Offlinemycocozm


Registered: 05/13/10
Posts: 181
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Re: LC question [Re: biologys]
    #13982279 - 02/17/11 11:37 PM (12 years, 11 months ago)

Thanks, but I'm not using a syringe I'm just free pouring.  Shouldn't change anything right?


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Invisiblebiologys
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Re: LC question [Re: mycocozm]
    #13982312 - 02/17/11 11:42 PM (12 years, 11 months ago)

good luck, dont pour too much and mess up your moisture....you're also taking much more of a risk of contam's in your jars and your LC by doing it that way...


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OfflineNew HooN
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Registered: 01/15/11
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Re: LC question [Re: mycocozm]
    #13982318 - 02/17/11 11:42 PM (12 years, 11 months ago)

i wouldnt free pore anything syringes are easy to come buy and very cheep...... just b shurr not to change your moisture level if u pore to much things will go bad


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Offlinemycocozm


Registered: 05/13/10
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Re: LC question [Re: New HooN]
    #13983673 - 02/18/11 09:36 AM (12 years, 11 months ago)

Thanks guys, but I got the lammy flow hood and careful is my middle name.  For serious.


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InvisibleDoc_T
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Re: LC question [Re: mycocozm]
    #13983696 - 02/18/11 09:41 AM (12 years, 11 months ago)

I stir for 15 mins once a day, and give LC jars as long as any other jars to colonize, 10-14 days.

Cockiness aside, don't think that equipment is a substitute for experience.
I don't have a flowhood, but I'd use syringes either way.


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Offlinemycocozm


Registered: 05/13/10
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Re: LC question [Re: Doc_T]
    #13983707 - 02/18/11 09:43 AM (12 years, 11 months ago)

Doc, do you stir them for 15 mins for just one day, or 15/day for several days?  And do you let the culture recover before syringing into your grain?


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Re: LC question [Re: mycocozm]
    #13983726 - 02/18/11 09:49 AM (12 years, 11 months ago)

Stir plate is on a timer, stirs once a day.
I usually shoot a couple test jars from each syringe right when I harvest.


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Offlinemycocozm


Registered: 05/13/10
Posts: 181
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Re: LC question [Re: Doc_T]
    #13983734 - 02/18/11 09:52 AM (12 years, 11 months ago)

Thanks Doc, but again, my primary question is unanswered.  The question is:

For HOW MANY DAYS do you let your jars stir (for 15 mins each day), before transferring? :thumbup: Thanks again.


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OfflineJoeker
"I know that I know nothing."


Registered: 01/01/11
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Re: LC question [Re: mycocozm]
    #13983795 - 02/18/11 10:07 AM (12 years, 11 months ago)

Edit: Doc answered your question again, no need for my interpretation now :thumbup:.


Edited by Joeker (02/18/11 10:23 AM)


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InvisibleDoc_T
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Re: LC question [Re: mycocozm]
    #13983800 - 02/18/11 10:08 AM (12 years, 11 months ago)

Already answered upthread, 10-14 days until I harvest. I shoot my first jars right then.
I tend to use LC to start grain masters for g2g. Then each jar is a test jar for itself.


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Offlinemycocozm


Registered: 05/13/10
Posts: 181
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Re: LC question [Re: Doc_T]
    #13984303 - 02/18/11 12:04 PM (12 years, 11 months ago)

Touche. Well kick ass then.  I'm gonna go take over the world (of beauty bark)


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Shop: North Spore Cultivation Supplies, Injection Grain Bag   Mushroom-Hut Liquid Cultures


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