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Offlineexumpei
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Registered: 02/16/11
Posts: 4
Loc: Vancouver, BC Flag
Last seen: 12 years, 7 months
First attempt. Is this a contamination?
    #13976201 - 02/16/11 09:44 PM (12 years, 11 months ago)

I've got these yellow things in all jars that show mycelium growth. Are these contaminations? Should I toss all jars out and start again?



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OfflineOut GrowV
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Registered: 09/03/09
Posts: 917
Loc: midwest
Last seen: 3 months, 28 days
Re: First attempt. Is this a contamination? [Re: exumpei]
    #13976246 - 02/16/11 09:51 PM (12 years, 11 months ago)

Yes those are contaminates and the jars are shot. Try again and use proper sterile procedures and mason jars.


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OfflinexMADSCIENTISTx
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Registered: 10/12/10
Posts: 71
Last seen: 8 years, 10 days
Re: First attempt. Is this a contamination? [Re: Out Grow]
    #13977320 - 02/17/11 01:51 AM (12 years, 11 months ago)

from the looks of those jars pretty scary contams. definitely get rid of jars and look through your procedure to avoid this in the future. there are no better teachers than trial and error.


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OfflineGravy Jones
The Fat Girl

Registered: 01/05/11
Posts: 27
Loc: Greendale
Last seen: 7 years, 7 months
Re: First attempt. Is this a contamination? [Re: xMADSCIENTISTx]
    #13978198 - 02/17/11 10:13 AM (12 years, 11 months ago)

yeah your gonna need to drop some cash to get mason jars and all the proper EQ. you will be very lucky to get fruits if you don't don't fallow a tek to the T.


--------------------
"The sun's not yellow, it's chicken!"~Bob Dylan


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Offlineunitoo1
Bending Minds


Registered: 12/02/10
Posts: 95
Last seen: 3 years, 5 months
Re: First attempt. Is this a contamination? [Re: Gravy Jones]
    #13979238 - 02/17/11 02:54 PM (12 years, 11 months ago)

ya +1 on the mason jars. plus a pressure cooker. 15psi.


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Offlineegodeathflux
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Registered: 02/02/10
Posts: 3,854
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Re: First attempt. Is this a contamination? [Re: unitoo1] * 1
    #13979261 - 02/17/11 02:59 PM (12 years, 11 months ago)

You don't need mason jars for BRF/PF tek at all, just read through the tek you're using a couple more times and flame that needle! Drinking glasses with foil/verm layer work just fine for plenty of members..

What tek were you following OP, how did you inoculate your jars? The more info you provide the more we can help you out.. :peace:


--------------------

"Atrophic interludes weave through my life far too often, for me to fight the biggest enemies"




"Standing on the corner of 5th and Vermouth"



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Offlineexumpei
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Registered: 02/16/11
Posts: 4
Loc: Vancouver, BC Flag
Last seen: 12 years, 7 months
Re: First attempt. Is this a contamination? [Re: egodeathflux]
    #13982510 - 02/18/11 12:14 AM (12 years, 11 months ago)

I'm using PF Tek for Simple Minds. And I indeed did not flame that needle this time. The thing is, I had 0th time with same Tek, when there was no sign of germination or contamination in any jar after one month and that time I did sterilize needle with flame. I thought that may be I killed spores with high temperature, so this time I sterilized needle only with alcohol.


Edited by exumpei (02/18/11 12:16 AM)


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OfflineOut GrowV
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Registered: 09/03/09
Posts: 917
Loc: midwest
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Re: First attempt. Is this a contamination? [Re: exumpei]
    #13983475 - 02/18/11 08:35 AM (12 years, 11 months ago)

Alcohol can not sterilize the inside of the needle.


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