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OfflineMeneerCactus
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Petris .... how much?
    #1396446 - 03/20/03 10:02 PM (13 years, 10 months ago)

High All,

I am wondering:
When I start isolating what is a good number of petris to work with.
10? 20? 30? 40? etc. . .

Any suggestion would be very nice !


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"Millions of years of evolution just to .... light up a joint"


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OfflinePaid
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Re: Petris .... how much? [Re: MeneerCactus]
    #1396457 - 03/20/03 10:08 PM (13 years, 10 months ago)

i start 2 form spores then when germinated i move each one onto 4 so from 2 i get 8 but normally lose a few to contams so the more the better.Especaily in the early days and when working with wild prints.

When making up the plates i always make 500ml of agar and fill about 30 petris as if kept in a clean place they last a long time and you can just use them as you need them.
Good luck mate


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InvisibleRoadkill
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Re: Petris .... how much? [Re: MeneerCactus]
    #1396489 - 03/20/03 10:22 PM (13 years, 10 months ago)

Depends on what your intent is.

If you want to work with alot of strains and species you will need quite a few.

I use between 40 to 100 petri dishes...most of the time.

But I work with alot of new strains.


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Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineMeneerCactus
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Re: Petris .... how much? [Re: Roadkill]
    #1396505 - 03/20/03 10:43 PM (13 years, 10 months ago)

High Roadkill,

I want to work with one strain to begin. Wanna see how it works, etc.
So would 20 or 30 be a good option?


--------------------
"Millions of years of evolution just to .... light up a joint"


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OfflinePaid
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Re: Petris .... how much? [Re: MeneerCactus]
    #1396510 - 03/20/03 10:50 PM (13 years, 10 months ago)

For just one strain you only need about 10 or so and only 2 or 3 to start the spores on i.e without peroxide.Then youll need a few more with peroxide once the spores germinate to do transpheres of the cleanist or most rhizomorphic sections to fresh peroxidated plates.
So 2 or 3 with out peroxide and 7 or 8 with peroxide,but you wont need these till about a week or more after you start the spores.

Or thats what i do.


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InvisibleRoadkill
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Re: Petris .... how much? [Re: MeneerCactus]
    #1396529 - 03/20/03 11:11 PM (13 years, 10 months ago)

I always make up 20 at a time.

10 should be enough for 1 strain...if you already have a culture.
More is better if you are starting up from spores.

I like to work with 4 petri dishes when starting with spores at first.
Then transfer the most aggressive mycelium to new plates.
Then transfer several more times til I get some really nice growth.

I never use peroxide agar...I have a very low contam rate. *crosses fingers*

I do make and use anti-bacterial agar when working with wild prints.

I have been using MEA and PDYA the most...PDYA is my personal favorite.

Your gonna love working with agar Eric.

good luck!


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinezeronio
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Re: Petris .... how much? [Re: Paid]
    #1396535 - 03/20/03 11:14 PM (13 years, 10 months ago)

Yes I also do it like this. 10 usually is more then enough for one strain. Later you might need more if you get some nasty contamn.


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OfflinePaid
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Re: Petris .... how much? [Re: Roadkill]
    #1396536 - 03/20/03 11:14 PM (13 years, 10 months ago)

What anti-bacterial agar do you use?(as in what antibiotic do you use?)do you mix your own or get the premade add water stuff?as i think i need some anti-bacterial agar for wild prints two.


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InvisibleRoadkill
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Re: Petris .... how much? [Re: Paid]
    #1396545 - 03/20/03 11:30 PM (13 years, 10 months ago)

I recieved some Gentamicin sulfate in liquid form, in vials from a friend and have been using that in MEA.

I bought some Gentamycin sulfate in powder form from Sporeworks but I haven't tried it out yet.

I make my own PDYA up.




--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflinePaid
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Re: Petris .... how much? [Re: Roadkill]
    #1396556 - 03/20/03 11:49 PM (13 years, 10 months ago)

Cool thaks i'll try Gentamycin sulfate because that junky looking bacteria always seems to sneak into at least one plate when i use spores.

And you never use peroxide hu....i'll keep my fingers crossed for you as well though you dont need the luck lol


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InvisibleChampion des Champignons
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Re: Petris .... how much? [Re: MeneerCactus]
    #1396829 - 03/21/03 03:49 AM (13 years, 10 months ago)

you can never have enough petris IME, especially once you get a good collection of strains going.
I have about 100 plates at the moment, 50 or more with stuff growing on them and almost as many empty ones, just in case:smirk:


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hmmm........


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InvisibleOlgualion
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Re: Petris .... how much? [Re: Champion des Champignons]
    #1396853 - 03/21/03 04:08 AM (13 years, 10 months ago)

I hear that!

My buddy has been getting 3 or 4 sleeves of plastic ones at a time; decided to do a thorough search for quantity and better prices.  He found a place that sells 25 sleeves of 20 for $57 after shipping!  Thats $2.28 per sleeve.  I can't wait till he gets them!

He has a few glass plates.  There was some guy on Ebay who was selling cases of 72 for like $80, but I haven't seen him in months :frown: 


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Study the past...
See the future...


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Invisiblepsyphon
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Re: Petris .... how much? [Re: Olgualion]
    #1397421 - 03/21/03 07:30 AM (13 years, 10 months ago)

Has anyone tried using jars? I was thinking of using wide form 4oz mason jars so that would be 12. They seem cheaper than glass plates and they can be pre-poured so it seems like it might be a good idea for a beginner. Any ideas on agar to water ratios for individual jars?

Thanks


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"The real voyage of discovery consists not in seeking new landscapes but in having new eyes."
- Marcel Proust

I wish you all ceaselessly flowing moments of happiness.


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OfflineBaby_Hitler
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Re: Petris .... how much? [Re: psyphon]
    #1397961 - 03/21/03 10:34 AM (13 years, 10 months ago)

I'm about to try it with some wide mouth half pint jars.


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InvisibleOlgualion
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Re: Petris .... how much? [Re: psyphon]
    #1397996 - 03/21/03 10:49 AM (13 years, 10 months ago)

Yes, this has been done, it 'should' work, so long as you follow the same procedures as you would with petris.

If you must pour the plates/ jars before sterilisation; I would make up a normal sized batch, then pour the agar, then sterilize. There will be alot of condensation! I couldn't tell you ratios for single jars though...

Also, take the ring part of your jar off. Use only the top and cover it with Al foil.


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See the future...


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OfflineMeneerCactus
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Re: Petris .... how much? [Re: Olgualion]
    #1398065 - 03/21/03 11:19 AM (13 years, 10 months ago)

Thanks for all you reply's :smile:


--------------------
"Millions of years of evolution just to .... light up a joint"


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InvisibleMushMushi
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Re: Petris .... how much? [Re: MeneerCactus]
    #1398092 - 03/21/03 11:36 AM (13 years, 10 months ago)

I've used many small jars, and even now, I think it's easier to work with them than with plastic petris.
I'm trying to buy a few glass petri dishes, but they are very expensive. I never had any problems with condensations.
You simply prepare the agar, pour it in the jars, pressure cook them, and voila!


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Offlinecomario2
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Re: Petris .... how much? [Re: MeneerCactus]
    #1398387 - 03/21/03 01:49 PM (13 years, 10 months ago)

i do 500 ml of mea, with 30 petris+ or -. i find it allows me to experiment withouot being excessive


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comario


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OfflineCultyVader
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Re: Petris .... how much? [Re: comario2]
    #1398567 - 03/21/03 02:59 PM (13 years, 10 months ago)

WHere do you guys get your agar? Any place where i can find it easily and cheaply?
Edit: I mean Agar-agar to make mea etc...


Edited by CultyVader (03/21/03 03:00 PM)


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InvisibleOlgualion
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Re: Petris .... how much? [Re: CultyVader]
    #1398706 - 03/21/03 04:08 PM (13 years, 10 months ago)

you can purchase agar-agar at health food stores. It either comes in powdered form or this 'crystal like' form (i'm sure there's a better description than that..). Then follow a recipe for PDA/ PDYA or MEA or DFA. All of which can probably be found somewhere on this site. My buddy has only used PD(Y)A and MEA of which he prefers PDA. Joshua has some beautiful cultures on DFA which he swears by. Dude will probably made up a batch of that type soon.


--------------------
Study the past...
See the future...


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Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Petri Dish, Potato Dextrose

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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