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InvisibleJavadog
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Registered: 05/03/10
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Re: jokefox's mutli media slants [Re: Barakanaten]
    #13800335 - 01/17/11 12:51 AM (13 years, 1 month ago)

Very interesting JF.

Thank you for sharing,

JD


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Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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OfflineEN1GMA
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Re: jokefox's mutli media slants [Re: Javadog]
    #13800465 - 01/17/11 01:35 AM (13 years, 1 month ago)

I want those!
and then I want this

Ahh I love Jurassic park...


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InvisibleWise Toad
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Re: jokefox's mutli media slants [Re: EN1GMA]
    #13951816 - 02/12/11 03:10 PM (13 years, 21 days ago)

Finally got around to reading all this, nice writups JF. I have a few questions reguarding equipment and use. Iv never used a culture slant before so my first project, to store the isolates I plan on making, will be using yours. Probably a noob question but why layer? Once you layer and cover with tape how do you introduce your culture or was that the agar broth? I followed the link to those microcentrifuge tubes, are these the right ones; the main reason I ask is that the volume is listed as 1.5ml, while in your instructions the first step is to fill the tubes with 1.5cc which would fill that much up. When injecting your culture into these slants do you store one isolated culture on several of these tubes so that you have a lot of backups in case one fails or you want to be able to withdraw several tubes worth(or do you even widraw)? How long do these last. Sorry if its a lot of questions, im too confused today

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InvisibleDJYoshaBYD
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Re: jokefox's mutli media slants [Re: Wise Toad]
    #13951908 - 02/12/11 03:25 PM (13 years, 21 days ago)

very informative and concise.. do I smell sticky? lol

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Invisiblejokefox
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Re: jokefox's mutli media slants [Re: DJYoshaBYD]
    #13951950 - 02/12/11 03:33 PM (13 years, 21 days ago)

i layer so i dont cut into the agar, i just suck the stuff up instead
the little vials i had were 3cc i think

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InvisibleWise Toad
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Re: jokefox's mutli media slants [Re: jokefox]
    #13952019 - 02/12/11 03:47 PM (13 years, 21 days ago)

Oh, so the top layer stays liquid such that you can aspirate, which one contains the genetics? Ill have to look around for some of those vials.

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Invisiblejokefox
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Re: jokefox's mutli media slants [Re: Wise Toad]
    #13952046 - 02/12/11 03:56 PM (13 years, 21 days ago)

add the agar broth after the agar layer has some mycelium on it
or just use an lc to inocculate the agar broth layer

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InvisibleWise Toad
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Re: jokefox's mutli media slants [Re: jokefox]
    #13952091 - 02/12/11 04:11 PM (13 years, 21 days ago)

Interesting, i'll post again when I have some made

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