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OfflinePaid
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Honey water tek question.
    #1394502 - 03/20/03 08:01 AM (14 years, 1 month ago)

I'm about to start some honey water to try to find a better way to do liquid inoculations and was just wondering if the h2o2 level would be the same as it is in agar?
Also wondering if i can start it from an agar weadge?or should i just peel the surface mycelium from the agar instead of the whole weadge?

I'm trying this as all the mycelium syringes ive made from agar seem to have little to no mycelium in so take a long time to then colanise the fruiting medium.Does any one have any tips of a better way to get the mycelium from the agar into a syringe apart from squirting water onto the agar scraping it and resucking it up?
Thanks in advance for any replys :-)


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InvisiblePsychoslut
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Re: Honey water tek question. [Re: Paid]
    #1394537 - 03/20/03 08:12 AM (14 years, 1 month ago)

add dextrose to your honey water, it helps sooooo much they will be ready for syringes in 5 days, also when you make the water, in like 2 days, you will notice floatys, that is wax and other impurities from the honey not a contam!!!! just multi spore inoc some jars. i made cambo jars with dextrose, 5 hours after innoc i could see growth, small growth growing from chunks of spores in the water, its really amazing.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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OfflineNiGGy
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Re: Honey water tek question. [Re: Psychoslut]
    #1394590 - 03/20/03 08:29 AM (14 years, 1 month ago)

can you make dextrose water? put a table spoon of dextrose in a pint jar. pressure cook. then innoculate? would that work as good?


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OfflineBruiser
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Re: Honey water tek question. [Re: Paid]
    #1394614 - 03/20/03 08:36 AM (14 years, 1 month ago)

Use corn syrup (dextrose) and water. Easier to see, less contams than honey. Works very well.

You can use a whole agar wedge. You needn't scrape the mycelium off. You can also use a piece of a mushroom to clone this way.
-Bruiser


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InvisiblePsychoslut
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Re: Honey water tek question. [Re: Bruiser]
    #1394645 - 03/20/03 08:42 AM (14 years, 1 month ago)

my freind enjoys using dextrose and honey at the same time, for the nutrition of the dextrose, and the anti-bacterial properties of honey, also of coarse h2o2


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Invisibledeanofmean
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Re: Honey water tek question. [Re: Paid]
    #1394659 - 03/20/03 08:46 AM (14 years, 1 month ago)

use about 1cc of 3% h2o2 per 8oz. honey water .
are you still using 20% h2o2 ? the ratio should be about 30:1 .
i don't see why you can't put agar in it . i do it with cardboard . works grate :laugh:
and, yeah i think dextrose works better . 


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Re: Honey water tek question. [Re: deanofmean]
    #1394700 - 03/20/03 08:58 AM (14 years, 1 month ago)

Cool thanks all i went  with the honey + detrose + peroxide and yep i still got the 17%  peroxide as i bought 5 liters lol so i think ill have it a few years yet :tongue:
I added .88ml to 500 ml of spring water which is the amount i do for agar.
I also did the whole agar weadge and added a couple of bits of glass to help cut it up when shaken.Wish me luck cos this is some Psilocybe semilanceata i finnaly got to germinate on agar so its my most important strain yet :grin:

5 shrooms each :tongue: to those i hadnt already rated :smile:


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Invisiblegeorge castanzaM
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Re: Honey water tek question. [Re: Bruiser]
    #1395106 - 03/20/03 12:00 PM (14 years, 1 month ago)

Quote:

Use corn syrup (dextrose) and water. Easier to see, less contams than honey. Works very well.
@4% ?


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Offlinedenarse
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Re: Honey water tek question. [Re: george castanza]
    #1395396 - 03/20/03 02:09 PM (14 years, 1 month ago)

can i innoculate dextrose water with a print? just scrape the spores through the hole?


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Re: Honey water tek question. [Re: denarse]
    #1396364 - 03/20/03 09:16 PM (14 years, 1 month ago)

Yes you can start spores or clones in dextrose water but if starting spores leave out the peroxide untill you see visable mycelium growth.


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Re: Honey water tek question. [Re: Paid]
    #1396385 - 03/20/03 09:27 PM (14 years, 1 month ago)

Una posted a great idea a while back. This is how dude uses it: Sterilize a half pint with a small amount of pre-soaked grain. Innoculate this with your wedge, spore syringe whatever. After colonised, he squirts a 30cc syringe of sterile H2O plus a little less than 1cc of H2O2 into the tape-covered hole in the lid. Shake jar and suck this back into the syringe.


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Re: Honey water tek question. [Re: Olgualion]
    #1396392 - 03/20/03 09:31 PM (14 years, 1 month ago)

did that work??? sounds easy enough


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Re: Honey water tek question. [Re: maddog151]
    #1396397 - 03/20/03 09:34 PM (14 years, 1 month ago)

most certainly does! It's dude's main technique for getting liquid cultures.


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Re: Honey water tek question. [Re: Olgualion]
    #1396444 - 03/20/03 10:00 PM (14 years, 1 month ago)

Sounds good.


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Re: Honey water tek question. [Re: Paid]
    #1396809 - 03/21/03 03:39 AM (14 years, 1 month ago)

andi if you really wan't Honey-water, just squirt a cc into a honey-water jar. Dude had been using h.w. for a few years, and started using the other method, since it is seems to yield less contamination. Depending upon the circumstances, h.w. is still used though.


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