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InvisibleBigBlackNinja
7hoe7

Registered: 02/25/03
Posts: 163
Loc: CA
Sterile Tissue Cloning Procedure
    #1391628 - 03/19/03 02:07 PM (14 years, 5 months ago)

i was wondering how successful anyone has been doing the Steril Tissue Cloning Procedure . has anyone been successeful cloning from the supermarket? i want to grow Portabellas and was wondering if you guys could give me tips on how i can clone these. also i have a coffee grinder but it is very old and dirty, is there anything i could subsitute for a coffee grinder, maybe a mortar and pestle (sp) or would smashing the tissue ruin it?

can you shroomers give me any advice on doing this? maybe a link or two on cultivating portabellas too?

anything is apriciated, thank you


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"your gunna have a bankroll so big its gunna look like your pockets got the mumps" -the mack


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Offlinekingkc
enthusiast
Registered: 04/25/00
Posts: 577
Last seen: 20 hours, 11 minutes
Re: Sterile Tissue Cloning Procedure [Re: BigBlackNinja]
    #1392029 - 03/19/03 04:16 PM (14 years, 5 months ago)

Just get the Portabella in your work area after you rinse it real well and dab it dry. Break the cap in half exposing the white insides.
Using a freshly flamed knife cut some of the tissue out and rapidly drop into a fresh plate or jar of grain.
There you have it.
Just be sure you follow protocol.
peace.


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Invisiblewhiterasta
Day careobserver
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Registered: 04/09/02
Posts: 1,780
Loc: Oregon
Re: Sterile Tissue Cloning Procedure [Re: BigBlackNinja]
    #1392048 - 03/19/03 04:22 PM (14 years, 5 months ago)

I have had recent success in cloning a wild A.campestris from a neighbors barn.I chose an unopened button and soaked it whole over nite in H2O2.I also prepared a jar of maple syrup/water liquid culture and covered the bottom of the jar with broken glass.I P/C'ed the liquid a nd the next day when it was cool I carefully sectioned the button in half with a heat sterilized knife,I sprayed the cut with H2O2 and carefully cut a small chunk from just above the gill line.I dipped this into peroxide and quickly dropped it into the liquid culture jar and sealed it.As soon as the peroxide bubbles disperse shake vigorously to cut up the chunk with the broken glass then let sit @ the correct temp(in the 80's for portos I believe) In a couple days you should see myc growing in the liquid jar.This can be drawn into a syringe and used to inoculate grain spawn.Good Luck  :wink:WR


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To old for this place


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InvisibleBigBlackNinja
7hoe7

Registered: 02/25/03
Posts: 163
Loc: CA
Re: Sterile Tissue Cloning Procedure [Re: whiterasta]
    #1392208 - 03/19/03 05:10 PM (14 years, 5 months ago)

thank you guys, any tek procedure suggestions or suggestions on the substrate to use? im a new grower just trying to learn as much as i can.
and is there anything i can put besides just grain that the mycelium will like to grow on more then just plain grain?


--------------------
"your gunna have a bankroll so big its gunna look like your pockets got the mumps" -the mack


Edited by BigBlackNinja (03/19/03 05:12 PM)


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Anonymous

Re: Sterile Tissue Cloning Procedure [Re: BigBlackNinja]
    #1392324 - 03/19/03 05:45 PM (14 years, 5 months ago)

So you can just make a paste of BRF at the bottom of a canning jar and pc at 15 pounds for 30 minute and instead of doing the blender thing can you just put the sterilized jar in a glove box and a mushroom and with a sterile knife cut open the stem take out the middle tissue of the shroom and add it to the jar. And dont add the h2o2. Will this work?


Edited by Anonymous (03/19/03 07:16 PM)


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Anonymous

Re: Sterile Tissue Cloning Procedure [Re: fee]
    #1392466 - 03/19/03 06:28 PM (14 years, 5 months ago)

Does anyone know?


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InvisibleBigBlackNinja
7hoe7

Registered: 02/25/03
Posts: 163
Loc: CA
Re: Sterile Tissue Cloning Procedure [Re: fee]
    #1392610 - 03/19/03 07:12 PM (14 years, 5 months ago)

sounds like people have been successful, but after i get mycelium, i don't know what to grow the shrooms on! what substrate do these shrooms like?


--------------------
"your gunna have a bankroll so big its gunna look like your pockets got the mumps" -the mack


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Anonymous

Re: Sterile Tissue Cloning Procedure [Re: BigBlackNinja]
    #1392629 - 03/19/03 07:17 PM (14 years, 5 months ago)

Can anyone answer my question


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OfflineAnnoA
Experimenter
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Registered: 06/18/99
Posts: 24,123
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Last seen: 1 month, 22 days
Re: Sterile Tissue Cloning Procedure [Re: fee]
    #1393046 - 03/19/03 09:33 PM (14 years, 5 months ago)

>Can anyone answer my question

If you don?t get any contaminations from the air or the tools, yes, it will work.


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Anonymous

Re: Sterile Tissue Cloning Procedure [Re: Anno]
    #1395088 - 03/20/03 11:54 AM (14 years, 5 months ago)

Thank you Anno I needed that


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