Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: OlympusMyco.com Olympus Myco Grow Bags   Kraken Kratom Kratom Capsules for Sale   Myyco.com Golden Teacher Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   North Spore Cultivation Supplies   Original Sensible Seeds High THC Strains   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Grow Bags   MagicBag.co All-In-One Bags That Don't Suck   Bridgetown Botanicals Bridgetown Botanicals

Jump to first unread post Pages: 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblehamloaf
Pork Block ®©™√
Male User Gallery


Registered: 12/23/09
Posts: 21,756
Loc: ation undisclosed.
Trusted Cultivator
OG Cultivator
Rattle Snake Venom.
    #13916002 - 02/06/11 01:46 PM (13 years, 3 months ago)

Is there any sort of processing of pure rattle snake venom from it's source that is needed, so that you can use the rattle snake venom in the break down of the hardened, exterior cellular wall of the spores for the intents of crossing strains of cubensis, specifically, that you know of?


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
Trusted Cultivator
OG Cultivator
Re: Rattle Snake Venom. [Re: hamloaf]
    #13916046 - 02/06/11 01:59 PM (13 years, 3 months ago)

It's heat sensitive, so needs to be milked into a sterile container under sterile flow hood conditions.  This is why lab grade venom is so expensive.  It's milked under sterile conditions and then freeze dried.

Thinking of heading down to the rattlesnake hunt in SW OK in the spring?
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
InvisibleCH HELL
Brain Sturgeon
Male User Gallery

Registered: 10/02/08
Posts: 6,610
Loc: mars Flag
Trusted Cultivator
Re: Rattle Snake Venom. [Re: RogerRabbit]
    #13916127 - 02/06/11 02:23 PM (13 years, 3 months ago)

If you have fresh spores the venom isn't always nessasary,  if you just want to "cross strains of cubensis".
CH

Extras: Filter Print Post Top
InvisibleN2DFlood
Fractalist
Male User Gallery


Registered: 11/10/10
Posts: 389
Re: Rattle Snake Venom. [Re: RogerRabbit]
    #13916171 - 02/06/11 02:31 PM (13 years, 3 months ago)

Quote:

RogerRabbit said:
It's heat sensitive, so needs to be milked into a sterile container under sterile flow hood conditions.  This is why lab grade venom is so expensive.  It's milked under sterile conditions and then freeze dried.

Thinking of heading down to the rattlesnake hunt in SW OK in the spring?
RR




Wow I've lived here on and off forever, never heard of a rattlesnake roundup, a little looking shows there are 5 of them between march and may...

How much per gram? hmm..
Look for my obituary this spring!

Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
Trusted Cultivator
OG Cultivator
Re: Rattle Snake Venom. [Re: N2DFlood]
    #13918001 - 02/06/11 09:01 PM (13 years, 3 months ago)

I used to live in Elk City, OK.  I owned a Harley dealership in the late 70s, early 80s.  We used to ride down every spring for the rattlesnake hunts.  There's always lots of beer and farmers market type stuff, plus it's fun to party with rednecks once in a while. :lol:
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
InvisibleNecco
Thread Killer
 User Gallery

Registered: 08/05/05
Posts: 608
Loc: west of a white house
Re: Rattle Snake Venom. [Re: RogerRabbit]
    #13919057 - 02/07/11 12:16 AM (13 years, 3 months ago)

Quote:

RogerRabbit said:
It's milked under sterile conditions and then freeze dried.




Just throwing this out there, but I believe collected venom could be sterilized by passing through a hydrophilic micron filter.

Any time you're paying for other people to risk their lives it will cost a fortune, as will buying something as (un)popular as venom, as will buying something that is only usually purchased by people with plenty of money.

Extras: Filter Print Post Top
Invisiblehamloaf
Pork Block ®©™√
Male User Gallery


Registered: 12/23/09
Posts: 21,756
Loc: ation undisclosed.
Trusted Cultivator
OG Cultivator
Re: Rattle Snake Venom. [Re: RogerRabbit]
    #13919736 - 02/07/11 06:40 AM (13 years, 3 months ago)

Quote:

RogerRabbit said:
It's heat sensitive, so needs to be milked into a sterile container under sterile flow hood conditions.  This is why lab grade venom is so expensive.  It's milked under sterile conditions and then freeze dried.

Thinking of heading down to the rattlesnake hunt in SW OK in the spring?
RR



Thanks for the information over the requirements of properly gathering laboratory grade rattle snake venom.  Now I'm picturing myself milking live snake venom in front of the flowhood.  How the heck do you think the snake is sterilized for entry into the laboratory?  I'm sure you don't do some shit like rub it down with an alcohol soaked paper towel or flame sterilize the son of a bitch.

Hunting rattle snakes in that part of the state and collecting laboratory grade venom is definitly on the agenda.  Got a few parteners who know and have family down in that section of the state.  Once I told them what laboratory grade rattle snake venom was worth, they suggested we go down and obtain some from private sources of known rattle snake nests.  What's in it for me is that I have a chance to add sterile, freeze dried, rattle snake venom into my arsenal of cultivation tools and additives.  With goals in sight to cross cubes using rattle snake venom to break down the hardened cellular wall of the cell.

However, not at any sort of collective gathering of general Oklahomans.  The rednecks and I have this thing about each other.  Seems like we always try to kill each other on sight.  :lol:


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
InvisibleN2DFlood
Fractalist
Male User Gallery


Registered: 11/10/10
Posts: 389
Re: Rattle Snake Venom. [Re: hamloaf]
    #13922245 - 02/07/11 05:19 PM (13 years, 3 months ago)

Most Okies would probably think mycology was the study of "dudes named Mike".  Country okies are generally friendly, just dont look or talk funny..heh, bring 6 point beer and make instant friends.  OK will be the last 3.2 state I'm sure.

I've seen snakes milked by biting the edge of a beaker through a film, i guess it thinks it's skin and they inject.  The snakes mouth would probably not have much contact with the inside of the glass.  At least, so it seems.

I would be seriously considering it were it not for the freeze drying and subzero storage requirements.  It is soooo almost doable.

Extras: Filter Print Post Top
Invisiblehamloaf
Pork Block ®©™√
Male User Gallery


Registered: 12/23/09
Posts: 21,756
Loc: ation undisclosed.
Trusted Cultivator
OG Cultivator
Re: Rattle Snake Venom. [Re: CH HELL]
    #13925935 - 02/08/11 06:07 AM (13 years, 3 months ago)

Quote:

CH HELL said:
If you have fresh spores the venom isn't always nessasary,  if you just want to "cross strains of cubensis".
CH



Thanks for joining my thread, CH.  How?


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
Trusted Cultivator
OG Cultivator
Re: Rattle Snake Venom. [Re: N2DFlood]
    #13926521 - 02/08/11 09:48 AM (13 years, 3 months ago)

Cubensis spores are generally compatible whether they came from the same cap or not.  Venom comes in handy with interspecies crosses, or if you have old spores which won't germinate, such as with Redboy.
RR

Quote:

N2DFlood said:
bring 6 point beer and make instant friends.  OK will be the last 3.2 state I'm sure.





I hadn't thought about that in fifteen years.  We'd get together on Saturday morning, roll a couple joints, and head to Gainsville for a few racks of Texas beer to stock the refrigerator with.  About the only other 'gladly forgotten' memory of OK is meeting someone and the first words out of their mouth is "have you accepted Jesus Christ as your personal Lord and savior"? :rolleyes:
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Invisiblelipa

Registered: 07/24/07
Posts: 2,684
Trusted Cultivator
Re: Rattle Snake Venom. [Re: RogerRabbit]
    #13926635 - 02/08/11 10:31 AM (13 years, 3 months ago)

Quote:

RogerRabbit said:
Cubensis spores are generally compatible whether they came from the same cap or not.  Venom comes in handy with interspecies crosses, or if you have old spores which won't germinate, such as with Redboy.
RR

Quote:

N2DFlood said:
bring 6 point beer and make instant friends.  OK will be the last 3.2 state I'm sure.





I hadn't thought about that in fifteen years.  We'd get together on Saturday morning, roll a couple joints, and head to Gainsville for a few racks of Texas beer to stock the refrigerator with.  About the only other 'gladly forgotten' memory of OK is meeting someone and the first words out of their mouth is "have you accepted Jesus Christ as your personal Lord and savior"? :rolleyes:
RR





This brings back memories for me HAHA! We did the same in NE OK except we would have to drive up through all the dry counties in Arkansas to the Missouri border where there is this huge gigantic warehouse of Alcohol and beer.:lol:

I can't stand bible thumpers! My freaking teachers would pull that crap on me in school.

Extras: Filter Print Post Top
InvisibleCH HELL
Brain Sturgeon
Male User Gallery

Registered: 10/02/08
Posts: 6,610
Loc: mars Flag
Trusted Cultivator
Re: Rattle Snake Venom. [Re: hamloaf]
    #13938774 - 02/10/11 10:51 AM (13 years, 3 months ago)

Quote:

hamloaf said:
Quote:

CH HELL said:
If you have fresh spores the venom isn't always nessasary,  if you just want to "cross strains of cubensis".
CH



Thanks for joining my thread, CH.  How?





Sorry haven't been able to get online,  but it looks like RR has already answered the question.:thumbup:
CH

Quote:

Cubensis spores are generally compatible whether they came from the same cap or not.  Venom comes in handy with interspecies crosses, or if you have old spores which won't germinate, such as with Redboy.
RR





Extras: Filter Print Post Top
Invisibletr20josh
Boomer
Male User Gallery

Registered: 06/05/10
Posts: 494
Re: Rattle Snake Venom. [Re: N2DFlood]
    #13950321 - 02/12/11 09:56 AM (13 years, 3 months ago)

Quote:

N2DFlood said:
OK will be the last 3.2 state I'm sure.




God damn Oklahoma piss water.... It doesn't even taste good, and your stomach is full before you get a buzz.  I can't wait to get out of this state.

Extras: Filter Print Post Top
Offlineslartibartfast
Stranger
Male User Gallery

Registered: 05/13/08
Posts: 203
Last seen: 4 years, 3 months
Re: Rattle Snake Venom. [Re: tr20josh]
    #13961371 - 02/14/11 01:45 PM (13 years, 3 months ago)

Does it make any difference as to the type of venom? the only local rattlesnake is a little guy called the massasauga and i believe that the venom from this snake is more neurotoxic than heamotoxic and i wonder if it would be less useful in breaking down cell walls and other things.
Please tell me they don't still hunt these wonderful creatures anymore at least not on a large scale like rattlesnake roundups. Just don't understand why we can't leave such useful (not that i think something needs to be useful for us to leave it in peace) creatures alone is beyond me.
Slart


--------------------
Pressure cooking shit to kill shit so i can knock it up with my shit to grow shit.
Trades welcome

Extras: Filter Print Post Top
Invisiblelazy bones
Stranger
 User Gallery


Registered: 04/22/10
Posts: 250
Re: Rattle Snake Venom. [Re: hamloaf]
    #13967019 - 02/15/11 11:23 AM (13 years, 3 months ago)

Quote:

hamloaf said:
Quote:

RogerRabbit said:
It's heat sensitive, so needs to be milked into a sterile container under sterile flow hood conditions.  This is why lab grade venom is so expensive.  It's milked under sterile conditions and then freeze dried.

Thinking of heading down to the rattlesnake hunt in SW OK in the spring?
RR



Thanks for the information over the requirements of properly gathering laboratory grade rattle snake venom.  Now I'm picturing myself milking live snake venom in front of the flowhood.  How the heck do you think the snake is sterilized for entry into the laboratory?  I'm sure you don't do some shit like rub it down with an alcohol soaked paper towel or flame sterilize the son of a bitch.

Hunting rattle snakes in that part of the state and collecting laboratory grade venom is definitly on the agenda.  Got a few parteners who know and have family down in that section of the state.  Once I told them what laboratory grade rattle snake venom was worth, they suggested we go down and obtain some from private sources of known rattle snake nests.  What's in it for me is that I have a chance to add sterile, freeze dried, rattle snake venom into my arsenal of cultivation tools and additives.  With goals in sight to cross cubes using rattle snake venom to break down the hardened cellular wall of the cell.

However, not at any sort of collective gathering of general Oklahomans.  The rednecks and I have this thing about each other.  Seems like we always try to kill each other on sight.  :lol:





Keep in mind many of the rattlesnakes collected for these hunts are harvested by dumping gallons of gas down a hole and waiting for something to come out. The snakes are also collected up to a year in advance and not fed/taken care of well during that time. They used to get stuffed with sawdust to bump up their weight. IE- their health is not at its best and I'd bet their mouths/fang shield area is not clean like it is supposed to be. Normally, the outside of the snake is not cleaned, just held up to a rubber coated glass and milked.

I'd recommend hooking up with a good venomous snake keeper willing to help you in this endeavor. Or if you really feel like risking it to milk a snake yourself, take a trip to a state where collecting a few snakes is legal. Collect a few, milk them, then return them to where you found them.

One last thing- if you are serious about milking them yourself, I'd really suggest trying first and getting some experience it on a species that won't kill you if you screw up. Copperheads immediately come to mind. Holding a venomous snake can be a bit more challenging that you would imagine, and their ability to move the fangs to inject something is incredible. If by chance you get struck by a rattlesnake there are some pretty serious complications that can arise, anaphylaxis due to the antivenom is one the biggest.

Sorry to rant, but I just wanted to share my experiences.

Extras: Filter Print Post Top
Invisiblehamloaf
Pork Block ®©™√
Male User Gallery


Registered: 12/23/09
Posts: 21,756
Loc: ation undisclosed.
Trusted Cultivator
OG Cultivator
Re: Rattle Snake Venom. [Re: lazy bones]
    #14018261 - 02/24/11 04:31 AM (13 years, 2 months ago)

What's up lazybones.  Don't sweat it on the rant my man.  Thanks for your words of caution and wisdom but a country boy can survive.  In no way shape or form do I plan on dumping gasoline down rattle snake holes to coax out rattle snakes snakes for the intended purpose of milking their venom.  Got a few friends who's families own land down in those sections of the state and know right where to go to find dens of these snakes.  My intentions are to cause the least amount of harm and stress to the creature as possible, given the unique circumstances as into the intended purposes of hunting the creatures. 

As precautionary measures to safety,  All rattle snake handling gear is to be purchased before hand as well as a few books about the subject are to be read.  Was also thinking of dropping the laboratorie's room temperature to 50 degrees causing a cold blooded reptile to become into a state paralysis for the sterile milking of the rattle snale venom.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
Trusted Cultivator
OG Cultivator
Re: Rattle Snake Venom. [Re: hamloaf]
    #14019030 - 02/24/11 09:49 AM (13 years, 2 months ago)

Quote:

Keep in mind many of the rattlesnakes collected for these hunts are harvested by dumping gallons of gas down a hole and waiting for something to come out. The snakes are also collected up to a year in advance and not fed/taken care of well during that time.




They sure as hell didn't do that when I was down there.  You have to sign up, get on a school bus, and they drive you to their designated location where you have to hunt in the presence of the judges.  It would be damned hard to carry a can of gas or a bag of snakes onto that bus. :shrug:
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Offlineshroomie_glen
RedHotPussyLiquor
Male User Gallery


Registered: 03/01/06
Posts: 4,296
Loc: Narf Carolina Flag
Last seen: 11 years, 6 months
Re: Rattle Snake Venom. [Re: RogerRabbit]
    #14038671 - 02/27/11 09:06 PM (13 years, 2 months ago)

I would have never thought that rattlesnake venom was that damn expensive.

I'm in the wrong goddamned business.


--------------------

No. No, man. Shit, no man.  I believe you'd get your ass kicked sayin' somethin' like that man.

Extras: Filter Print Post Top
Invisiblehamloaf
Pork Block ®©™√
Male User Gallery


Registered: 12/23/09
Posts: 21,756
Loc: ation undisclosed.
Trusted Cultivator
OG Cultivator
Re: Rattle Snake Venom. [Re: shroomie_glen]
    #14041353 - 02/28/11 11:33 AM (13 years, 2 months ago)

I'm not sure by what volume, but labrotory grade rattle snake venom is worth more than a kelo of cocaine.  The last price I heard a kelo of cocaine going for was 15,000 dollars, conservatively.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
Trusted Cultivator
OG Cultivator
Re: Rattle Snake Venom. [Re: hamloaf]
    #14043354 - 02/28/11 04:36 PM (13 years, 2 months ago)

Sigma had it for around $300/gram last time I bought it.  That big can had a little tiny vial in it, which held the gram.
RR



--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Offlinealbertz
Stranger
Registered: 07/10/10
Posts: 17
Last seen: 10 years, 3 months
Re: Rattle Snake Venom. [Re: RogerRabbit]
    #14046229 - 02/28/11 11:49 PM (13 years, 2 months ago)

you can try cobra venom also, buy Cobroxin at any drugstore, like 20 dollars for a bottle..and there is a prescription strength also...

Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
Trusted Cultivator
OG Cultivator
Re: Rattle Snake Venom. [Re: albertz]
    #14047063 - 03/01/11 06:11 AM (13 years, 2 months ago)

Cobroxin isn't nearly potent enough to work.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
InvisiblePinback
Stranger
 User Gallery

Registered: 07/20/02
Posts: 836
Loc: Europe
Re: Rattle Snake Venom. [Re: RogerRabbit]
    #14048396 - 03/01/11 12:57 PM (13 years, 2 months ago)

Have the results in the Holliday paper ever been verified with some sort of genetic analysis? I can only find one reference to the method in the primary literature, and it is that paper.
Considering the doubts regarding Hollidays Dictyophora paper, I think this is a valid question.

Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
Trusted Cultivator
OG Cultivator
Re: Rattle Snake Venom. [Re: Pinback] * 1
    #14051183 - 03/01/11 09:08 PM (13 years, 2 months ago)

Ask him.

In my case, it enabled monokaryotic mycelium to crawl across 20 year old spores that refused to germinate, and grab a mate.  As soon as the monokaryotic mycelium reached the area of spores, two strains of rhizomorphic dikaryotic mycelium emerged.  The same experiment without snake venom had failed several times, as had every other procedure I tried, such as pressure hydration, heat, ultrasonic vibration, and a few others.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Offlineshroomie_glen
RedHotPussyLiquor
Male User Gallery


Registered: 03/01/06
Posts: 4,296
Loc: Narf Carolina Flag
Last seen: 11 years, 6 months
Re: Rattle Snake Venom. [Re: RogerRabbit]
    #14051207 - 03/01/11 09:11 PM (13 years, 2 months ago)

RR you perpetually blow my mind.
And I mean this in the gayest of ways:
I love you dude.


--------------------

No. No, man. Shit, no man.  I believe you'd get your ass kicked sayin' somethin' like that man.

Extras: Filter Print Post Top
InvisiblePinback
Stranger
 User Gallery

Registered: 07/20/02
Posts: 836
Loc: Europe
Re: Rattle Snake Venom. [Re: RogerRabbit]
    #14052266 - 03/02/11 12:03 AM (13 years, 2 months ago)

Well, mating compatible monokaryons is not really a good way to test if the method is valid. If the spores are non-viable, why would a proteolytic toxin bring them back to life? I know the standard answer is "because they weaken the cell wall", but neither this, nor why the mycelium would combine with the nuclei (why assume the genetic material would be intact if the toxin can break down a cell wall?) instead of consuming them has been shown.

No evidence that shows that the method works; sketchy theory as to why it would work; earlier dubious publications. These kinds of things make my warning bells go off...

Extras: Filter Print Post Top
Invisiblelipa

Registered: 07/24/07
Posts: 2,684
Trusted Cultivator
Re: Rattle Snake Venom. [Re: Pinback]
    #14060539 - 03/03/11 02:43 PM (13 years, 2 months ago)

Maybe because the enzyme breaking down the cell wall doesn't work on the nucleus. In some commercial applications they use Novozyme -234 Which is basically glucanase and chitinase. Why would these effect the nucleus? I am also curious as to how the nuclei made it into the vicinity of the spores in RR method. I am not saying it didn't work but there has to be some kind of attractant that made them come together.  Usually they us PEG from what I have read. They use it to lower th electronegativity of the protoplasts because they usually carry a negative charge and repel each other. Maybe the Snake venom does this as well

RR - When you made the agar up for this was it soft or firm? Does this matter?

Lipa

Edited by lipa (03/03/11 02:45 PM)

Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
Trusted Cultivator
OG Cultivator
Re: Rattle Snake Venom. [Re: Pinback]
    #14060862 - 03/03/11 03:41 PM (13 years, 2 months ago)

Quote:

Pinback said:
No evidence that shows that the method works; sketchy theory as to why it would work; earlier dubious publications. These kinds of things make my warning bells go off...




No problem.  It's much easier to stick to tried and true methods already proved.  I've offered pictures of the original plates, and the cross was a red-spored cubensis, but not nearly as red spored as the original Redboy spores. This is in line with expectations, since the monokaryotic mycelium was isolated from a normal-colored spore print.  This is anecdotal evidence, but it's the evidence there is.  Furthermore, once the process was complete, the experiment was repeated with monokaryotic mycelium from the cross, which resulted in spores much closer to the original Redboy.

The only evidence I have to offer is that I made dozens of attempts to germinate the old Redboy spores with no success.  After having already tried everything I knew to do, I bought the snake venom, and used it in agar.  The result was as I said above.  Perhaps the monokaryotic mycelium digested the spores and somehow got the genetic material that way.  I don't know.  Fungi and how they work their processes are still a mystery to us, certainly to me at least.  I'm still learning, and experimenting in an effort to learn more.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Invisiblecyanara
jedi in training
Male


Registered: 12/22/09
Posts: 1,205
Loc: your grow closet
Re: Rattle Snake Venom. [Re: RogerRabbit]
    #14070229 - 03/05/11 10:23 AM (13 years, 2 months ago)

looks like we need to take a trip to Idaho boys.. go get our own Venom.. from McCall to Grand Gene sawtooth area it is chalk full of those things.

Extras: Filter Print Post Top
Invisiblefngbronco
Monkey Man
Male


Registered: 09/26/10
Posts: 2,877
Re: Rattle Snake Venom. [Re: cyanara]
    #14087010 - 03/08/11 02:33 PM (13 years, 2 months ago)

You guys want rattlers I'll just swing by the old pen here in Boise. I'd totally be down to try and milk one of those suckers! Is the venom processed in any way prior to use?


--------------------
I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek

Extras: Filter Print Post Top
Invisiblefungitobewith
Fungi To Be With
 User Gallery


Registered: 07/15/07
Posts: 754
Loc: Rolling Stone, PA/Some Pl... Flag
Re: Rattle Snake Venom. [Re: fngbronco]
    #14139281 - 03/17/11 08:29 PM (13 years, 2 months ago)

Look at some of these prices :eek: some are not to bad but damn.
http://www.venomsa.com/prices_list/index.htm

I think I would prefer to leave the snake milking up the the professionals, this site sells all types of venom, with the cheapest being, Monocle Cobra @ $100.00 a gram

Do you really need to go out and milk a wild snake? The time effort and money spent seems to me to be a waste? Planning a trip like that...costly :thumbdown: in my opinion.

RR how long have you had that same container of venom?


--------------------

Check out my
My Trade List  :opensign:

Check out my
Available Strain List  :opensign:


I want your old coins, :pm: me if you have something you want to trade.



Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
Trusted Cultivator
OG Cultivator
Re: Rattle Snake Venom. [Re: fungitobewith]
    #14140088 - 03/17/11 10:23 PM (13 years, 2 months ago)

It was all used up long ago.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Invisiblemycoelf
Agent Of Chaos
Male User Gallery


Registered: 06/26/09
Posts: 555
Loc: hyperspace Flag
Re: Rattle Snake Venom. [Re: RogerRabbit]
    #14140124 - 03/17/11 10:29 PM (13 years, 2 months ago)

:bow2:


--------------------
Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:

Extras: Filter Print Post Top
Invisiblehamloaf
Pork Block ®©™√
Male User Gallery


Registered: 12/23/09
Posts: 21,756
Loc: ation undisclosed.
Trusted Cultivator
OG Cultivator
Re: Rattle Snake Venom. [Re: mycoelf]
    #15456286 - 12/02/11 08:16 PM (12 years, 5 months ago)

Yeahso, to anybody who gave a rat's behind about this thread, I just wanted to report, NOTHING culminated out of this.  My buddy got me all hyped to go hunt rattle snakes and obtain their venom for a profit so, I made this thread, obtained the information needed to be proceedin' and, haven't heard back from him since. 

If I was more bad-ass, I'd go hunting the snakes by myself but, I think it wise to use the buddy system when trying to capture live rattle snakes.  When I get into a more comfortable lifestyle, I'll go at this again.  Most likely in my hometown of San Diego where there's no shortage of rattle-snakes or people syco enough to hunt some rattleres (my brother comes to mind).


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
InvisibleJavadog
Continuing along
Male User Gallery


Registered: 05/03/10
Posts: 7,385
Loc: USA Flag
Trusted Cultivator
Re: Rattle Snake Venom. [Re: hamloaf]
    #15457164 - 12/02/11 11:36 PM (12 years, 5 months ago)

I know my way around reptiles bro. 

When I was a kid I bet my brother $5 that I would become a herpatologist.

He got no money, as he did not become a marine either.

We do have a Costa Rican Rosy Boa though....little fucker bites...a RB that bites!

;0)

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

Extras: Filter Print Post Top
Offlinemarkalbob
Stranger....than most
Male User Gallery
Registered: 03/12/10
Posts: 233
Loc: Madison, WI
Last seen: 3 years, 4 months
Re: Rattle Snake Venom. [Re: Javadog]
    #15471665 - 12/05/11 08:49 PM (12 years, 5 months ago)

would lyticase work as well? 

We used to use that to "crack" yeast and bust up the cell wall.....it would be a shit-ton cheaper than rattlesnake venom


--------------------
Trade list Updated

Haves:  Shiitake West Wind and Snow Cap, Blue and pearl oyster, store-cloned brown beech mushroom, Field and Forest Maitake, Nameko, 2 wild-cloned shaggymane cultures, H. erniaceus and americanum, and SRA.

Wants:  Blewitt, stone-producer print
PM if you'd like to swap

Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 2 months
Trusted Cultivator
OG Cultivator
Re: Rattle Snake Venom. [Re: markalbob]
    #15473423 - 12/06/11 07:32 AM (12 years, 5 months ago)

Experiment and find out.  That's the only way we learn.  I'm sure there's plenty of agents which will work, it's just a matter of trial and error to find the right ones.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Offlinemarkalbob
Stranger....than most
Male User Gallery
Registered: 03/12/10
Posts: 233
Loc: Madison, WI
Last seen: 3 years, 4 months
Re: Rattle Snake Venom. [Re: RogerRabbit]
    #15473918 - 12/06/11 10:05 AM (12 years, 5 months ago)

I'm not hybridizing, or at that stage yet....if you are, it may be worth looking at.  lyticase was for breaking yeast cell walls, which I'm guessing are fairly similar to spore walls, so it seems a good candidate.  I'd also guess it is maybe 1/5 the cost of venom, or less even.


--------------------
Trade list Updated

Haves:  Shiitake West Wind and Snow Cap, Blue and pearl oyster, store-cloned brown beech mushroom, Field and Forest Maitake, Nameko, 2 wild-cloned shaggymane cultures, H. erniaceus and americanum, and SRA.

Wants:  Blewitt, stone-producer print
PM if you'd like to swap

Extras: Filter Print Post Top
Offlineglideher
Stranger
Registered: 04/06/18
Posts: 9
Last seen: 2 years, 10 months
Re: Rattle Snake Venom. [Re: markalbob]
    #27386287 - 07/13/21 05:53 PM (2 years, 10 months ago)

is rattle snake venom same as cobra venom strain?


--------------------
dlg gliders

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: OlympusMyco.com Olympus Myco Grow Bags   Kraken Kratom Kratom Capsules for Sale   Myyco.com Golden Teacher Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   North Spore Cultivation Supplies   Original Sensible Seeds High THC Strains   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Grow Bags   MagicBag.co All-In-One Bags That Don't Suck   Bridgetown Botanicals Bridgetown Botanicals


Similar ThreadsPosterViewsRepliesLast post
* Rattle snake venom dosage Solipsis 608 3 02/23/20 10:25 AM
by murderlabz
* questions regarding PE6, rattle snake venom, and home attempts. serotonin storm 902 3 08/28/12 08:09 PM
by weetsie
* Snake Venom Into Hybrids
( 1 2 3 4 5 all )
dumbfounded1600 23,869 86 03/01/11 09:21 PM
by CH HELL
* Snake venom hybridization method debunked (?)
( 1 2 all )
Pinback 4,103 25 11/19/18 10:11 PM
by Peter65199
* Snake venom agar tek
( 1 2 all )
Mycostotle 2,281 37 09/27/20 01:19 PM
by Solipsis
* Snake Venom *vs* Chitinase 24sevenZed 470 3 05/05/20 11:00 AM
by 24sevenZed
* Snake venom for "stabilization"? Solipsis 436 2 07/22/20 08:24 AM
by Doc9151
* Alternative to snake venom in hybridizing.... Justamaninafish 2,467 4 08/21/14 09:01 AM
by Mr. Alien

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
11,203 topic views. 1 members, 2 guests and 5 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.04 seconds spending 0.008 seconds on 14 queries.