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Wizzy
In Tha Hizzy


Registered: 11/09/10
Posts: 852
Loc: Void Center
Last seen: 11 years, 2 months
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Mycelium Transfer BRF Questions? (UPDATED! Colonizing!)
#13884027 - 01/31/11 07:25 PM (12 years, 11 months ago) |
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I poop in my hand and smear it on my chest. Call it Shitk's Vapo Rub
Edited by Wizzy (03/15/11 12:08 AM)
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
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Re: Mycelium Transfer BRF Questions? [Re: Wizzy]
#13884092 - 01/31/11 07:38 PM (12 years, 11 months ago) |
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Cake to cake transfers don't work.
Filtered gas exchange is required for all colonizing substrates. In brown rice flour cakes, the dry vermiculite barrier is the gas exchange filter.
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Smokey-hitz
Geocacher



Registered: 01/02/11
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Re: Mycelium Transfer BRF Questions? [Re: Wizzy]
#13884117 - 01/31/11 07:42 PM (12 years, 11 months ago) |
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no polyfill needed for brf
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Wizzy
In Tha Hizzy


Registered: 11/09/10
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Re: Mycelium Transfer BRF Questions? [Re: hamloaf]
#13884284 - 01/31/11 08:09 PM (12 years, 11 months ago) |
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I poop in my hand and smear it on my chest. Call it Shitk's Vapo Rub
Edited by Wizzy (03/15/11 12:08 AM)
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N2DFlood
Fractalist



Registered: 11/10/10
Posts: 389
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Re: Mycelium Transfer BRF Questions? [Re: Wizzy]
#13884402 - 01/31/11 08:31 PM (12 years, 11 months ago) |
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I've done cake to cake transfers but i use bags and not jars.
I would try it, especially if someone told me it couldn't be done...just on general principle.
If i could get a good enough piece of mycelium extracted clean, i'd bust it up with some sterile water and make a ghetto LC and inject that for possibly better inoculation. Works with grains quite well, cakes... mmm dunno.
One way to find out, IMO.
Just depends on your resources, backups, options, and resolve.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
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Re: Mycelium Transfer BRF Questions? [Re: Wizzy]
#13884464 - 01/31/11 08:44 PM (12 years, 11 months ago) |
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Quote:
Wizzy said:
Quote:
hamloaf said: Cake to cake transfers don't work.
Filtered gas exchange is required for all colonizing substrates. In brown rice flour cakes, the dry vermiculite barrier is the gas exchange filter.
Yeah, but for my cakes... there won't be a dry verm layer as obviously it will prevent the mycelium dropped on it from colonizing the substrate below. I have lids with polyfill and self-sealing silicone inoculation points, and you're saying this still won't work? I've heard of people having success with cake to cake transfers, I mean in theory shouldn't it work the same exact way as dropping an agar wedge in there? That doesn't work for BRF either?
You didn't mention you'll not be applying the dry vermiculite barrier. In that case you will definitely need a means of gas exchange to your brown rice flour cakes.
Nobodies saying you can't do a cake to cake transfer just that they generally don't work. If I was to be all compulsive about doing a cake to cake transfer, I'd drop a piece of colonized brown rice flour cake onto agar to have the option of transferring clean mycelium away from contaminates.
Unless you'r just looking to research, it's bad advice to just do something that's been proven more times then not to fail then to try mostly unproven methods in defiance of more aware and seasoned cultivators.
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skuzzy1232
:))



Registered: 05/31/10
Posts: 1,460
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Re: Mycelium Transfer BRF Questions? [Re: N2DFlood]
#13884471 - 01/31/11 08:45 PM (12 years, 11 months ago) |
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iyour right iv done this . on a cake that stalled i filled a bowl with water used a used needle and scraped while pulling the handle and your left with mycelium in the syrenge the more white in the needle the better it colonizes a little faster than spores. i hope this is what you meant it works.
i just had back surgery and am highly medicated right now so im alittle woosey
-------------------- http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463 Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .
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SomeGuy
I feel better now :)


Registered: 04/18/10
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Re: Mycelium Transfer BRF Questions? [Re: hamloaf]
#13884476 - 01/31/11 08:47 PM (12 years, 11 months ago) |
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N2DFlood
Fractalist



Registered: 11/10/10
Posts: 389
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Re: Mycelium Transfer BRF Questions? [Re: SomeGuy]
#13884550 - 01/31/11 09:01 PM (12 years, 11 months ago) |
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I'm sorry Hamloaf, it wasnt about defying anyone, especially a proven knowledgeable cultivator such as yourself. I imagine a lot of your knowledge comes from experience, research, and experimentation. I see where I implied defiance.. but it was aimed at the absolute, not at you personally.
It's more about learning from experience. If i tried and failed multiple times then i'd know...if i never tried... i'd always wonder.
Of course, i wouldn't try if it was say, my only cake, or if i could imagine a better way to accomplish the same goal.
The only guarantee is that if one does not try, it will certainly not work.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
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Re: Mycelium Transfer BRF Questions? [Re: SomeGuy]
#13884573 - 01/31/11 09:05 PM (12 years, 11 months ago) |
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I'm fully aware of the transferring of mycelium from a brown rice flour cake using water as the mycelial transfer medium.
Nowhere in the OP was anything mentioned of a mycelial transfer using water as the mycleial transfer medium. Nor was there any mention of the non utilization of a vermiculite barrier.
So i stand by my words that it would never be a good idea to transfer a piece of a cake onto another cake, like a grain to grain transfer. Just like how the OP implies in in his original post.
A better way to achieve a mycelial transfer using water as your transfer medium is agar's "GRAIN LC".
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Mycelium Transfer BRF Questions? [Re: N2DFlood]
#13884775 - 01/31/11 09:39 PM (12 years, 11 months ago) |
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There's better ways to accomplish the same goal.
-------------------- You make it all possible. Doesn't it feel good?
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Wizzy
In Tha Hizzy


Registered: 11/09/10
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Re: Mycelium Transfer BRF Questions? [Re: hamloaf]
#13884822 - 01/31/11 09:46 PM (12 years, 11 months ago) |
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Quote:
hamloaf said: Nor was there any mention of the non utilization of a vermiculite barrier.
Yeah I did, I said:
Quote:
Wizzy said: Yeah, but for my cakes... there won't be a dry verm layer
But look Hamloaf dude I know you know what you're talking about, you seem like you're smart as hell from all your other posts on here dude so if you say it won't work I'm willing to accept that it will probably result in failure but now you're starting to sound like maybe there's a chance it COULD work if I don't use a verm layer and let the glass jars breathe through polyfill? Assuming I do everything in my sterilization procedures and everything else correctly?
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Mycelium Transfer BRF Questions? [Re: Wizzy]
#13884826 - 01/31/11 09:47 PM (12 years, 11 months ago) |
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It's not impossible, just unlikely and impractical and a waste of time running down the wrong path.
-------------------- You make it all possible. Doesn't it feel good?
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N2DFlood
Fractalist



Registered: 11/10/10
Posts: 389
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Re: Mycelium Transfer BRF Questions? [Re: Doc_T]
#13884880 - 01/31/11 09:56 PM (12 years, 11 months ago) |
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the "grain LC" which is actually neither in this case, is probly your best bet.
If a tissue transfer did work i imagine it would be very slow.
Even better, moving forward, keep a true and tested LC on hand, they store easily, they're easy to make, and if you have a PC they're your best bet for avoiding this situation in the future with risky transfers and imbalancing water content in your jars.
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Wizzy
In Tha Hizzy


Registered: 11/09/10
Posts: 852
Loc: Void Center
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Re: Mycelium Transfer BRF Questions? [Re: N2DFlood]
#13906562 - 02/04/11 05:55 PM (12 years, 11 months ago) |
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I poop in my hand and smear it on my chest. Call it Shitk's Vapo Rub
Edited by Wizzy (03/15/11 12:08 AM)
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
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Re: Mycelium Transfer BRF Questions? [Re: Wizzy]
#13906635 - 02/04/11 06:08 PM (12 years, 11 months ago) |
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Still not clear on what exactly it is that you did. Did you cut away a piece of colonized cake away and transfer the piece of colonized cake onto a freshly sterilized receiving cake or did you transfer the mycleium from the mother cake into receiving jars using sterile water and syringes as your transfer medium?
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Wizzy
In Tha Hizzy


Registered: 11/09/10
Posts: 852
Loc: Void Center
Last seen: 11 years, 2 months
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Re: Mycelium Transfer BRF Questions? [Re: hamloaf]
#13906790 - 02/04/11 06:34 PM (12 years, 11 months ago) |
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I poop in my hand and smear it on my chest. Call it Shitk's Vapo Rub
Edited by Wizzy (03/15/11 12:09 AM)
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Mycelium Transfer BRF Questions? [Re: Wizzy]
#13906863 - 02/04/11 06:52 PM (12 years, 11 months ago) |
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Very cool and interesting, indeed. I'm glad this is working so far. Keep up the good work. I apologize for sounding so damning.
You're not out of the woods yet. Give them jars another 3-4 more days to come back contaminate free before calling your procedure 100% viable. 5 days is up to how long any invader spore present in your substrate or mother spawn'll take to germinate and rear it's ugly head.
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Wizzy
In Tha Hizzy


Registered: 11/09/10
Posts: 852
Loc: Void Center
Last seen: 11 years, 2 months
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Re: Mycelium Transfer BRF Questions? [Re: hamloaf]
#13906873 - 02/04/11 06:53 PM (12 years, 11 months ago) |
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Quote:
hamloaf said: Very cool and interesting, indeed. I'm glad this is working so far. Keep up the good work. I apologize for sounding so damning.
You're not out of the woods yet. Give them jars another 3-4 more days to come back contaminate free before calling your procedure 100% viable. 5 days is up to how long any invader spore present in your substrate or mother spawn'll take to germinate and rear it's ugly head.
Sweet man thanks for the heads up! I'll let you know what happens bro
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Wizzy
In Tha Hizzy


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Posts: 852
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Re: Mycelium Transfer BRF Questions? [Re: Wizzy]
#13930907 - 02/08/11 11:18 PM (12 years, 11 months ago) |
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I poop in my hand and smear it on my chest. Call it Shitk's Vapo Rub
Edited by Wizzy (03/15/11 12:09 AM)
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