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Offlinekindkesey
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Can i incoulate a 2nd time??
    #1384381 - 03/17/03 02:40 PM (13 years, 8 months ago)

I have been succesful many times before. I,ve incoulated my jars almost 2 weeks ago and still nothing. I often see growth in 4-5 day with my incubator set at 86. I have also used this same batch of substate before. But the bad news is that my incubater temp reached 95 one night, i think i killed the spores. Can i inoculate them a 2nd time???


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OfflineTheeShadow2169
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Re: Can i incoulate a 2nd time?? [Re: kindkesey]
    #1384443 - 03/17/03 02:56 PM (13 years, 8 months ago)

You might want to sterilize the jars agian in the pressure cooker. Other then that they should be fine. Let me know how they turn out.

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OfflineExtravagantDream
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Re: Can i incoulate a 2nd time?? [Re: kindkesey]
    #1384449 - 03/17/03 02:57 PM (13 years, 8 months ago)

If you feel like it wont grow than go for it. Just remember to be sterile.


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OfflineExtravagantDream
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Re: Can i incoulate a 2nd time?? [Re: TheeShadow2169]
    #1384462 - 03/17/03 03:00 PM (13 years, 8 months ago)

i wouldnt really do that, if you're going to PC again, might as well be new substrate.


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Offlinekindkesey
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Re: Can i incoulate a 2nd time?? [Re: ExtravagantDream]
    #1384492 - 03/17/03 03:08 PM (13 years, 8 months ago)

I have opened a jar and took a wiff, it still smells good and grainy if i use new substrate i'll have to cook a new batch. Should i waste a sringe to try it? has anyone tryed this ?


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Edited by kindkesey (03/17/03 03:15 PM)


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Offlinemetalchimp
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Re: Can i incoulate a 2nd time?? [Re: kindkesey]
    #1384545 - 03/17/03 03:27 PM (13 years, 8 months ago)

if you really want to innoc again pc them again first!

better safe than sorry!


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OfflineBigJohnson
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Re: Can i incoulate a 2nd time?? [Re: metalchimp]
    #1384601 - 03/17/03 03:42 PM (13 years, 8 months ago)

Quote:

if you really want to innoc again pc them again first!

better safe than sorry!




I'll second that.


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OfflineExtravagantDream
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Re: Can i incoulate a 2nd time?? [Re: metalchimp]
    #1384648 - 03/17/03 03:56 PM (13 years, 8 months ago)

If it is grain, I would not PC again. Although I havnt tryed it, I can only imagine how it will turn out. When grains are over cooked they turn into a mush.


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Invisibledeanofmean
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Re: Can i incoulate a 2nd time?? [Re: kindkesey]
    #1385056 - 03/17/03 05:36 PM (13 years, 8 months ago)

95*F would not have killed cubensis spores . mycelium death occurs 106*f .

is this the same strain that you had success with before ? if the spores are old, or if not properly hydrated before inoculation, it sometimes takes longer to germinate .

also, depending on humidity, and how long the temp was at 95, the substrate may have dried out .

it may be best to start over, and if you have enough jars, give those another week before giving up on them .



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Invisiblegeorge castanzaM
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Re: Can i incoulate a 2nd time?? [Re: deanofmean]
    #1385631 - 03/17/03 07:59 PM (13 years, 8 months ago)

kramer says to pitch em and start over with new spores and substrate.he claims that when the jars don't start and grow agressivly that flushes are often disappointing if successful at all :shocked:



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Offlinekindkesey
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Re: Can i incoulate a 2nd time?? [Re: george castanza]
    #1387547 - 03/18/03 10:37 AM (13 years, 8 months ago)

Well this is what i'm going to do. I will pc 6 jars tonight and wait a few more day and if i still see anything in the other 6. If i dont, i'll incoulate them a 2nd time. I'll keep my fingers crossed and hope it works. If any one else has any more sugestions please give me your 2 cents!! thanks


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OfflineAmanita_Dreamer
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Re: Can i incoulate a 2nd time?? [Re: george castanza]
    #1401698 - 03/22/03 11:35 PM (13 years, 8 months ago)

Quote:

kramer says to pitch em and start over with new spores and substrate.he claims that when the jars don't start and grow agressivly that flushes are often disappointing if successful at all :shocked:

 



As much as I hate to admit it george is right...Why fuck around with a chance of contams???Just start over the odds are much better and much more worth your time. :grin: 


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*DELETED* [Re: kindkesey]
    #1401920 - 03/23/03 03:17 AM (13 years, 8 months ago)

Post deleted by Script

Reason for deletion: .



Edited by PJDIDDLE (05/09/10 01:07 AM)


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Offlinekindkesey
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Re: Can i incoulate a 2nd time?? [Re: PJDIDDLE]
    #1433052 - 04/06/03 08:45 AM (13 years, 8 months ago)

Well we did it !! All 12 jars have started to grow, The 6 that were pc a second time took off a little slower then the 6 we did nothing to. I have a strong feeling the syringe used the first time had very little or no spores in it!! (spore works) I have contacted the vendor and they wer happy to sent replacements.

thanks for your help, shroomies

stay kool


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Invisibletripndicular
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Re: Can i incoulate a 2nd time?? [Re: kindkesey]
    #1433150 - 04/06/03 11:19 AM (13 years, 8 months ago)

1 st off ..... 95*F will not "kill" spores or mycelium .
2nd .... why potentially waste more inoc materials , when you are not for certain that this batch is just being shy , or perhaps your jars are infected . So why not make up some new stuff in a few days and inoc it ?
Yes you could inoc a second time but why risk wating that material ?
Good Luck


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OfflineNirvana
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Re: Can i incoulate a 2nd time?? [Re: tripndicular]
    #1433504 - 04/06/03 03:11 PM (13 years, 8 months ago)

What negative temperature kills spores? 0 degrees celsius or is it into the minuses?

Heat Death - 106f
Cold Death - ????


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OfflineHowardo
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Re: Can i incoulate a 2nd time?? [Re: Nirvana]
    #1433515 - 04/06/03 03:17 PM (13 years, 8 months ago)

my friend innoculated a 2nd time after there was no growth. they busted out with thick whitey goodness. yeah, be sterile


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Amazon Shop for: ½ Pint Jars, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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