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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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InvisibleQuebecShroomer
Shroom Hunter

Registered: 03/16/03
Posts: 41
Loc: In a BIG Amanita
Sterilisation Problem
    #1382596 - 03/17/03 05:09 AM (14 years, 14 days ago)

Contaminant are my worst ennemy.
I'Ve read several hour on shroom cultivation, but never found the best and costless sterile technique to kill these F"!*&?%$ bacteria. I try to grow shroom from last summer to now without sucess. What can I do?


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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Sterilisation Problem [Re: QuebecShroomer]
    #1382615 - 03/17/03 05:15 AM (14 years, 14 days ago)

You need to provide more details. What process are you using? What exactly is the problem that you are having?


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Sterilisation Problem [Re: dog]
    #1382633 - 03/17/03 05:21 AM (14 years, 14 days ago)

Dog is correct , we need a little more info to "try" to help . There are so many variables when it comes to contams .
At which stage of the game are you get the ickies ?
But ask yourself one important question ............... Have I truly taken all measures with in my abilities to keep lab clean ?
Good Luck


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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InvisibleQuebecShroomer
Shroom Hunter

Registered: 03/16/03
Posts: 41
Loc: In a BIG Amanita
Re: Sterilisation Problem [Re: dog]
    #1382637 - 03/17/03 05:23 AM (14 years, 14 days ago)

I use the BRF & verm. subtrat. For sterilisation, I use an old Presto , let the jars in for about an hour. When it cool down, I inoculate them with this tek: 3 holes in lid
with aluminium foil over it, them with my syringe inoculate them by the 3 holes.
I can see mycelium growing about 5 to 6 day after.
The problem that I always have, is that after 2 weeks , green mold are showing up and take control over mycelium.
How can I avoid that?


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OfflinePooPs
What's thisfor???

Registered: 03/05/03
Posts: 4,510
Loc: Stirrin up the pile!
Last seen: 7 years, 11 months
Re: Sterilisation Problem [Re: QuebecShroomer]
    #1382643 - 03/17/03 05:29 AM (14 years, 14 days ago)

When you fill your jars. do you wipe down the last 1/2 inch of the jar lid ( the thread part. ) then fill the jar with dry verm.

Then PC in the presto, and inject, wipe down your needle between each injection with alc, or flame. keep trying. you will get it.


--------------------


-----------
Sniff, Sniff... What's that smell???... ohhhhh.!!
------------------


Pot Free for another : nevermind.. never made it..


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Offlinebluesky
mushroom cowboy

Registered: 09/04/02
Posts: 561
Last seen: 13 years, 8 months
Re: Sterilisation Problem [Re: QuebecShroomer]
    #1382645 - 03/17/03 05:29 AM (14 years, 14 days ago)

You do flame the needle right?


--------------------
You're my blue sky, you're my sunny day,
Lord you know it makes me high when you turn your love my way. Turn your love my waaaaaay, Yea.
-Richard (Dickey) Betts


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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Sterilisation Problem [Re: QuebecShroomer]
    #1382647 - 03/17/03 05:29 AM (14 years, 14 days ago)

If you are PC'ing your jars for an hour at 15 PSI then we can rule out substrate contamination. Therefore the contamination is occuring either during inoculation or incubation.

After inoculation do you seal your jars? My friend recommends taping the needle holes and leaving the jar sealed until 50%-60% colonization.

Do you make your own syringes?


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Sterilisation Problem [Re: QuebecShroomer]
    #1382652 - 03/17/03 05:31 AM (14 years, 14 days ago)

Clean the area you store them in real good , use some lysol or pinesol cleaner and wipe down all surfaces . Then do you have those holes in the lids sealed up real good ? Then each and every time you go to touch those jars , make sure you clean your hands real good with antibac soap , do not forget to scrub under finger nails .
Are you sure you are achieving right temp and pressure in the cooker , this could also be why you get "delayed" contams . If you did not kill any and all nasties in yoor mix , they can come to life down the road when conditions are just right for them . Maybe you could add 15 mins to cook time .
You could also try wearing a mask when  you handle your jars .
Rule of thumb ................."CLEANLINESS IS NEXT TO MUSHINESS !"
Good Luck
"May The Mushroom Goddess Bless All Your Endeavors !"  :cool: 


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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InvisibleQuebecShroomer
Shroom Hunter

Registered: 03/16/03
Posts: 41
Loc: In a BIG Amanita
Re: Sterilisation Problem [Re: PooPs]
    #1382654 - 03/17/03 05:32 AM (14 years, 14 days ago)

What do you mean by `` Wipe down``?

(sorry for this question, I'm a French speaker that try to do his best to understand english)


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OfflineJssMthrFcknChrst
Son of the LordGod Almighty

Registered: 10/12/02
Posts: 446
Loc: Vatican City
Last seen: 12 years, 6 months
Re: Sterilisation Problem [Re: tripndicular]
    #1382657 - 03/17/03 05:34 AM (14 years, 14 days ago)

Haven't you seen the lysol commercials. No matter how many bottles of pinesol you use, it won't get as clean as lysol because pinesol doesn't disinfect.


--------------------



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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Sterilisation Problem [Re: JssMthrFcknChrst]
    #1382665 - 03/17/03 05:38 AM (14 years, 14 days ago)

My bottle says it "disinfects" , personally I use a product called "dispatch" , is a hospital grade disinfectant , the shit will kill just about anything , even spores . But for the average Joe .....either, ither, or will do the trick . Use whatever you can , but try to provide the cleanest enviro possible , your mushies will love you for it !


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Edited by tripndicular (03/17/03 05:39 AM)


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Offlinebluesky
mushroom cowboy

Registered: 09/04/02
Posts: 561
Last seen: 13 years, 8 months
Re: Sterilisation Problem [Re: JssMthrFcknChrst]
    #1382667 - 03/17/03 05:38 AM (14 years, 14 days ago)

LOL!!!!! I told my GF that Lysol was better than Pinesol, but she said that they were the some thing.


--------------------
You're my blue sky, you're my sunny day,
Lord you know it makes me high when you turn your love my way. Turn your love my waaaaaay, Yea.
-Richard (Dickey) Betts


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Offlinebluesky
mushroom cowboy

Registered: 09/04/02
Posts: 561
Last seen: 13 years, 8 months
Re: Sterilisation Problem [Re: tripndicular]
    #1382670 - 03/17/03 05:40 AM (14 years, 14 days ago)

WOW! Where do you get this stuff?


--------------------
You're my blue sky, you're my sunny day,
Lord you know it makes me high when you turn your love my way. Turn your love my waaaaaay, Yea.
-Richard (Dickey) Betts


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OfflinePooPs
What's thisfor???

Registered: 03/05/03
Posts: 4,510
Loc: Stirrin up the pile!
Last seen: 7 years, 11 months
Re: Sterilisation Problem [Re: JssMthrFcknChrst]
    #1382673 - 03/17/03 05:40 AM (14 years, 14 days ago)

Utilise tous les precautions possibles.

Essuie l'eguille entre chaque injections avec de l'alcool, encore mieux, utilise une flamme et chauffe l'eguille,

lave tes mains avant de toucher tes pots.

Esque tu as un incubateur? mon francais n'est pas trop bon, mais je peux t'aider un peu si tu veux.



--------------------


-----------
Sniff, Sniff... What's that smell???... ohhhhh.!!
------------------


Pot Free for another : nevermind.. never made it..


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InvisibleQuebecShroomer
Shroom Hunter

Registered: 03/16/03
Posts: 41
Loc: In a BIG Amanita
Re: Sterilisation Problem [Re: bluesky]
    #1382675 - 03/17/03 05:41 AM (14 years, 14 days ago)

What kind of incubator can i use to be sure that no bacteria can introduce my precious jars?


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InvisibleQuebecShroomer
Shroom Hunter

Registered: 03/16/03
Posts: 41
Loc: In a BIG Amanita
Re: Sterilisation Problem [Re: QuebecShroomer]
    #1382680 - 03/17/03 05:44 AM (14 years, 14 days ago)

I do not yet have a 100% effective incubator.

Merci poops d'essayer de m'aider, vos conseils me sont vraiment utile.


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Offlinebluesky
mushroom cowboy

Registered: 09/04/02
Posts: 561
Last seen: 13 years, 8 months
Re: Sterilisation Problem [Re: QuebecShroomer]
    #1382681 - 03/17/03 05:44 AM (14 years, 14 days ago)

An aquarium. One that you can put fish in. They are water-tight! :grin:


--------------------
You're my blue sky, you're my sunny day,
Lord you know it makes me high when you turn your love my way. Turn your love my waaaaaay, Yea.
-Richard (Dickey) Betts


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OfflinePooPs
What's thisfor???

Registered: 03/05/03
Posts: 4,510
Loc: Stirrin up the pile!
Last seen: 7 years, 11 months
Re: Sterilisation Problem [Re: QuebecShroomer]
    #1382683 - 03/17/03 05:45 AM (14 years, 14 days ago)

2 contenants rubbermaid, lave les avec du javex, couvre avec une couverture.

ca devrait suffire.


--------------------


-----------
Sniff, Sniff... What's that smell???... ohhhhh.!!
------------------


Pot Free for another : nevermind.. never made it..


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Sterilisation Problem [Re: bluesky]
    #1382686 - 03/17/03 05:46 AM (14 years, 14 days ago)

Conections my man ....connections . Have a friend in the med industry that provides me with all kinds of neat shit I use for mushie projects . Pre sterilized water is my fave , free autoclav , surgical garments ie caps , masks , gowns , gloves . Got to have connections !


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinebluesky
mushroom cowboy

Registered: 09/04/02
Posts: 561
Last seen: 13 years, 8 months
Re: Sterilisation Problem [Re: tripndicular]
    #1382691 - 03/17/03 05:49 AM (14 years, 14 days ago)

mmmmmmmmm........pre-steralized water..........(drool)


--------------------
You're my blue sky, you're my sunny day,
Lord you know it makes me high when you turn your love my way. Turn your love my waaaaaay, Yea.
-Richard (Dickey) Betts


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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