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 Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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 andymc
cocoa beetles from zanzibar
 
Registered: 11/25/09
Posts: 2,395
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 Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference?  [Re: tibberous]
#13820696 - 01/20/11 02:03 PM (13 years, 2 months ago) |
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I can see that you do understand how this all works, but remember that noobs are going to be reading/searching this thread years from now, and they may not come away with the right idea.
They may think "if flame sterilization is good, and alcohol sanitization is good, then combining them must be better". Most people want to do the best job they can, so it's easy to make those kinds of logical mistakes. Look at the way some people think "if 70% IPA is good, 90% must be better", but they're wrong.
Other people are lazy, and might read this and think simply alcohol sanitizing a syringe is good enough, but if you, tibberous, own flame sterilization gear (as you seem to) and use it (as you seem to) then you clearly know that alcohol isn't good enough on its own.
Sometimes, making sure people get the right information from this forum is tricky business.
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How I make spore prints
Trade List
My flow hood
If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper" -odds
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Base Icks
Registered: 03/19/10
Posts: 6,191
Loc: Shroomshire 
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: tibberous]
#13821585 - 01/20/11 04:30 PM (13 years, 2 months ago) |
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Quote:
tibberous said:
Quote:
andymc said:
Quote:
pockets le peu said:
besides that just keep the needle covered with an alcohol soaked nap and wipe between innoculations.
 NO. Don't touch your sterilized needle with anything, not even alcohol-soaked whatevers.
Save the alcohol for sanitizing your gloves. Just flame-sterilize for each jar. It's not difficult, so I don't understand why various people around here are so resistant to it. Is it laziness?
Same reason I said - because a hot needle will melt a huge hole in plastic bag or a tyvek lid. I happen to flame sterilize then wipe - it works fine. If you can use a hot needle, great - but you don't have too. It doesn't matter whether you incinerate the bacteria or kill it or just wipe it off with a rag - it's fine. I'll bet half the contams people think they are getting from the inoc aren't even from it - there either coming from bad syringes or not pressure cooking the jars right. I haven't had a contam before bulk since I started PC'ing 90 minutes (not even off agar or LC's)
Yeah a red hot lightsaber needle  is going to mess up inoculation ports and the like. Heres how you solve that riddle. After flaming the needle end to end red hot place it back in your GB/SAB ASAP. Once in there, squeeze out a drop of two of solution.
Problem solved. Needled cooled to way less than the boiling point of water.
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 TheCannabisSativa
Stranger
Registered: 09/20/10
Posts: 408
Last seen: 9 years, 1 month
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: Base Icks]
#13821992 - 01/20/11 05:55 PM (13 years, 2 months ago) |
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I always thought/heard it was important to use alcohol as the flame your sterilizing with but everybody is saying it dosnt matter right...?
I think I remember reading something about butane being a dirty flame but must not be that important.....I'll stick with alcohol though.
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andymc
cocoa beetles from zanzibar
Registered: 11/25/09
Posts: 2,395
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: TheCannabisSativa]
#13822085 - 01/20/11 06:18 PM (13 years, 2 months ago) |
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Quote:
TheCannabisSativa said:
I always thought/heard it was important to use alcohol as the flame your sterilizing with but everybody is saying it dosnt matter right...?
I think I remember reading something about butane being a dirty flame but must not be that important.....I'll stick with alcohol though.
This  me for a second, but you're asking about using an alcohol lamp to flame things, am I right?
Yes, absolutely use an alcohol lamp to sterilize, if you have one. Propange/butane work fine too. Just don't wipe rubbing alcohol on things after you flame-sterilize them 
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How I make spore prints
Trade List
My flow hood
If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper" -odds
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roaddog
turnip
Registered: 09/29/09
Posts: 2,288
Loc: third rock from the sun 
Last seen: 3 years, 11 months
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: andymc]
#13822149 - 01/20/11 06:30 PM (13 years, 2 months ago) |
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You know what I dont flame sterilize, anything, ever. I dont really use alcohol ether.Well I take that back, I might flame a stored syringe. But If I just made it, I inoculate and go.But I always tell new folks to flame, because It is a good procedure to follow. But Its hell on needle tips.
Edited by roaddog (01/20/11 06:39 PM)
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TheCannabisSativa
Stranger
Registered: 09/20/10
Posts: 408
Last seen: 9 years, 1 month
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: roaddog]
#13822952 - 01/20/11 08:53 PM (13 years, 2 months ago) |
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Quote:
This me for a second, but you're asking about using an alcohol lamp to flame things, am I right?
Yes, absolutely use an alcohol lamp to sterilize, if you have one. Propange/butane work fine too. Just don't wipe rubbing alcohol on things after you flame-sterilize them
Yes I'm talking about using alcohol to flame things, but I use an upside down glass that somebody changes/lights alcohol when I'm ready for each jar. I wasn't talking about using alcohol instead of or after flaming....I always wipe with alcohol after innoculting then flame the needle for the next jar.
sorry for the response delay...
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Iride1203
Human
Registered: 02/13/17
Posts: 57
Last seen: 3 years, 10 months
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 Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: TheCannabisSativa]
#24215226 - 04/03/17 12:34 PM (7 years, 14 days ago) |
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I just did some spore prints today and only used alcohol to wipe my scalpel before removing the stem. I forgot to flame sterilize, leading me to put in big letters on my SAB "Flame sterilize". Question is- are these spore prints now contaminated and shouldn't be used? I have more fruits on the way so I am able to go go another round with making a new print.
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Mad Season
hookers and blackjack
Registered: 09/16/12
Posts: 12,666
Loc: Canada 
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: Iride1203]
#24215257 - 04/03/17 12:44 PM (7 years, 14 days ago) |
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Expect every spore print to be contaminated, and as such agar is the best for germination. Also loops/swabs > scalpels..
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Iride1203
Human
Registered: 02/13/17
Posts: 57
Last seen: 3 years, 10 months
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: Mad Season]
#24215311 - 04/03/17 01:03 PM (7 years, 14 days ago) |
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Ok I kinda figured since the mushroom itself is exposed to the outside elements especially in a SGFC. I also just got finished reading the Magic Mushrooms growing guide by Quaesitor and it seems as if you can still take a uncontaminated square in a contaminated agar dish (early on) and transfer the healthy cube into a new agar dish? I'm soon going to get into the agar stuff and isolate my strain off a tissue sample of these spore prints I'm making into syringes to cut down transfers. So Thanks for feedback
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Iride1203
Human
Registered: 02/13/17
Posts: 57
Last seen: 3 years, 10 months
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: Iride1203]
#24215316 - 04/03/17 01:06 PM (7 years, 14 days ago) |
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And as to my first question you believe these prints will still be ok to use?
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Mad Season
hookers and blackjack
Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: Iride1203]
#24215592 - 04/03/17 03:04 PM (7 years, 14 days ago) |
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Yeah they will be ok to use.
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mrmazdarx9
Pffffttt
Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 6 months
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: Mad Season]
#24215639 - 04/03/17 03:16 PM (7 years, 14 days ago) |
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Hello 6 years ago 
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COCA GROWERS come here and share your knowledge
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 Click here for UK trades
need some supplies in the UK check Here or PM me
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Iride1203
Human
Registered: 02/13/17
Posts: 57
Last seen: 3 years, 10 months
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: mrmazdarx9]
#24215779 - 04/03/17 04:00 PM (7 years, 14 days ago) |
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Quote:
mrmazdarx9 said:
Hello 6 years ago
Why is that a bad thing? I'm new to the forum I just found the appropriate thread to best suit my question... Sorry if it's a pain in the ass for you.
Edited by Iride1203 (04/03/17 04:01 PM)
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mrmazdarx9
Pffffttt
Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 6 months
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: Iride1203]
#24215794 - 04/03/17 04:06 PM (7 years, 14 days ago) |
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Its annoying to everyone to bump shit old threads and any noobs coming along could read outdated info thinking its upto date. When using the search function correctly you would have answered your question. Instead of using it as a quick search function click on the search our forum button, which then allows you to shorten the search to more modern knowledge by reducing to less than 3 years old and click on the trusted cultivators button so you only get the info what the highly knowledgeable guys like mad season above.
--------------------
COCA GROWERS come here and share your knowledge
COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me
UK OTD uk members chat
UK supplies and trade OTD place to chat shit
Right Here
If you use "SWIM" you should DROWN
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Iride1203
Human
Registered: 02/13/17
Posts: 57
Last seen: 3 years, 10 months
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: mrmazdarx9]
#24215904 - 04/03/17 04:49 PM (7 years, 14 days ago) |
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Oh ok good info thanks. My bad
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mrmazdarx9
Pffffttt
Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 6 months
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: Iride1203]
#24215927 - 04/03/17 04:59 PM (7 years, 14 days ago) |
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No worries the search bar when used correctly is actually really good its just knowing how to use it thats the issue, the quick search thing brings stuff up from 15 years ago some of the shit it brings up is funny as fuck.
--------------------
COCA GROWERS come here and share your knowledge
COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me
UK OTD uk members chat
UK supplies and trade OTD place to chat shit
Right Here
If you use "SWIM" you should DROWN
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CapnZ
Dimensional explorer
Registered: 12/22/16
Posts: 1,420
Loc: In the Mystic
Last seen: 5 months, 30 days
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: Ekyldog]
#24215982 - 04/03/17 05:25 PM (7 years, 14 days ago) |
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Quote:
Ekyldog said:
Propane torches set on low flame work great for sterilizing as well.
I love my bunsen burner. 
Propane torch on low. Yeah baby I'll never use anything else. Just squeeze out a few drops of fluid between flames to cool the needle. It will steam a bit with the first drop or 2, but once you have liquid drops coming out you are good to go. And like hamloaf said there are billions of spores in there....
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Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...
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Iride1203
Human
Registered: 02/13/17
Posts: 57
Last seen: 3 years, 10 months
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Re: Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference? [Re: mrmazdarx9]
#24216439 - 04/03/17 08:46 PM (7 years, 14 days ago) |
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Quote:
mrmazdarx9 said:
No worries the search bar when used correctly is actually really good its just knowing how to use it thats the issue, the quick search thing brings stuff up from 15 years ago some of the shit it brings up is funny as fuck.
Yeah I just checked it out when you sent me that last message. Very efficient thanks man
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