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Invisiblejokefox
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Agar broth, throw out your crap LCs * 5
    #13781184 - 01/13/11 03:53 PM (13 years, 17 days ago)

jokefox's Agar Broth

Agar broth is my media of choice for Liquid cultures, and live syringes.
Its more stable this way, and i find its less prone to contaminations,
also , theres no way for your sugar to burn.


This is your final product



Here it is after colonized,




here we go kids..


Gather your supplies, you will need.-
-5 jars/with lids (1 pint works well)
-Measuring glass
-Agar (MEA,PDA or whatever you use) enough for 500ML water
-Pressure cooker
-water- use tap water, (wasting money on fancy water is not necessary at all for this hobby)

Step one- making your agar,


measuring out 500ml water, put 250ml water in each of the 2 jars
then add your agar mix (dry)

Pressure cook for 30 minutes at 15psi

let your agar cool before handling.
(you will only need 1 jar for the next step ,
so go ahead and date and label the agar jar you will store for later use
and throw that bitch in your fridge

Step two- turning the agar , into liquid life

you will need the last 3 jars, 1 jar of your agar from step 1, measuring glass
and water

add 250ml of water into each jar,

then split the molten agar 3 ways,


(if you want a thicker LC, that can hold bubbles- only split the agar two ways)
for an extra boost, add a pinch of pollen to each jar
pressure cook for 13psi for 30 minutes, let cool


there you have it , nice golden AB (agar broth) ready for LC's , live syringes, or MMS (multi media slants)



Related teks
Peterthinks


Edited by jokefox (02/13/11 10:17 PM)


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Invisiblevirus1824
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Re: Agar broth, throw out your crap LCs [Re: jokefox]
    #13781204 - 01/13/11 03:56 PM (13 years, 17 days ago)

and what do u use to counter the solidification process which is the usual purpose of using agar?


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Offlineredline
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Re: Agar broth, throw out your crap LCs [Re: jokefox]
    #13781229 - 01/13/11 03:59 PM (13 years, 17 days ago)

Oh so this is the tek that I saw someone talking about in another thread. Looks sweet I might have to try that out when I make my first LC soon.


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Invisiblejokefox
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Re: Agar broth, throw out your crap LCs [Re: virus1824]
    #13781238 - 01/13/11 04:00 PM (13 years, 17 days ago)

Quote:

virus1824 said:
and what do u use to counter the solidification process which is the usual purpose of using agar?




if you read threw it ,
you will see how the agar is diluted then pc'ed a second time

that is what stops it from going hard again


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Invisiblesicknick
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Re: Agar broth, throw out your crap LCs [Re: jokefox]
    #13781250 - 01/13/11 04:02 PM (13 years, 17 days ago)

I hate trying to find light malt for my lc... I'm going to give this a try on my upcoming project..

You said to add a pinch of pollen for a boost? Why is that?


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Invisiblejokefox
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Re: Agar broth, throw out your crap LCs [Re: sicknick]
    #13781273 - 01/13/11 04:05 PM (13 years, 17 days ago)

Quote:

sicknick said:
I hate trying to find light malt for my lc... I'm going to give this a try on my upcoming project..

You said to add a pinch of pollen for a boost? Why is that?




ive read pollen helps and adds a really good amount of usables for the mycelia,
it works pre good , im just out at the moment
if you want your lc thicker, play with the ratios, you can get it so that the Agar broth
hold bubbles, at around 1/2 diluted
ive had great success up to 1/4 diluted,
but i love the 1/3 diluted ,


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Invisiblevirus1824
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Re: Agar broth, throw out your crap LCs [Re: jokefox]
    #13781295 - 01/13/11 04:09 PM (13 years, 17 days ago)

and why would i use agar and pc a second time when i can use the other ingredients except agar and have great results 2. What does the agar add in your opinion that's worth the extra trouble.


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Invisiblevirus1824
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Re: Agar broth, throw out your crap LCs [Re: sicknick]
    #13781308 - 01/13/11 04:10 PM (13 years, 17 days ago)

Quote:

sicknick said:
I hate trying to find light malt for my lc... I'm going to give this a try on my upcoming project..

You said to add a pinch of pollen for a boost? Why is that?




Try finding a specialty shop that sells stuff with brewing beer. That's where i find my light malt extract for 10 bucks a kilogram


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Invisiblesicknick
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Re: Agar broth, throw out your crap LCs [Re: jokefox]
    #13781316 - 01/13/11 04:11 PM (13 years, 17 days ago)

Quote:

jokefox said:
Quote:

sicknick said:
I hate trying to find light malt for my lc... I'm going to give this a try on my upcoming project..

You said to add a pinch of pollen for a boost? Why is that?




ive read pollen helps and adds a really good amount of usables for the mycelia,
it works pre good , im just out at the moment
if you want your lc thicker, play with the ratios, you can get it so that the Agar broth
hold bubbles, at around 1/2 diluted
ive had great success up to 1/4 diluted,
but i love the 1/3 diluted ,




Nice! I've never heard that before.. ill definitly give this a go..
I've kinda steered clear of LCs before but I like this, nice job :thumbup:


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Invisiblejokefox
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Re: Agar broth, throw out your crap LCs [Re: virus1824]
    #13781324 - 01/13/11 04:12 PM (13 years, 17 days ago)

Quote:

virus1824 said:
and why would i use agar and pc a second time when i can use the other ingredients except agar and have great results 2. What does the agar add in your opinion that's worth the extra trouble.





it adds body

when you use this for grains , instead of soaking in like water,
it will sit more on the top
witch will allow for a faster colonization, since the mycelium will be able to "glide"
over the surface you created


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InvisibleWise Toad
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Re: Agar broth, throw out your crap LCs [Re: jokefox]
    #13781366 - 01/13/11 04:17 PM (13 years, 17 days ago)

Now I can get rid of my crap honey and just use multipurpose PDA. Nice writeup:rockon:


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Offlineacidonion
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Re: Agar broth, throw out your crap LCs [Re: jokefox]
    #13781385 - 01/13/11 04:20 PM (13 years, 17 days ago)

I have yet to do a LC, I'm currently on my first grow, jars are colonizing. I have been thinking about doing a LC for my next grow, deff worth a shot IMO.


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Invisiblejokefox
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Re: Agar broth, throw out your crap LCs [Re: acidonion]
    #13781397 - 01/13/11 04:21 PM (13 years, 17 days ago)

Thanks for the love guys :todcasil:


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InvisibleOzzy
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Re: Agar broth, throw out your crap LCs [Re: jokefox]
    #13781468 - 01/13/11 04:31 PM (13 years, 17 days ago)

Nice writeup Jokey:awethumb:

I for some reason wasn't able to get lc's to go(only tried once though).

I will definitely give this a shot

thread has been saved for future reference.

Thanks


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Invisiblejokefox
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Re: Agar broth, throw out your crap LCs [Re: Ozzy] * 1
    #13781552 - 01/13/11 04:44 PM (13 years, 17 days ago)

just like you say man
Quote:

EXTERRMINATE!!!!!  EXTERRMINATE!!!!!  EXTERRMINATE!!!!!




and this is what i got,

i love it, ive aways had horrible time with honey
and with this, its just easy ,

i also like to replace water in spore syringes in it, just make sure you test the batch


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InvisibleShadOWCrack
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Re: Agar broth, throw out your crap LCs [Re: Ozzy]
    #13781609 - 01/13/11 04:55 PM (13 years, 17 days ago)

Word!!

this may be a noob ? but, what are the bubbles for??


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Invisiblejokefox
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Re: Agar broth, throw out your crap LCs [Re: ShadOWCrack]
    #13781624 - 01/13/11 04:57 PM (13 years, 17 days ago)

GE inside the core of the liquid

better thicker growth

usually you will get a matt of myelium on top floating

when you get the bubbles, you get chucks of mycelium threw out the liquid


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Re: Agar broth, throw out your crap LCs [Re: jokefox]
    #13781671 - 01/13/11 05:04 PM (13 years, 17 days ago)

Ohhhh i thought i fucked up when i did my first set of agar jars, it had bubbles 
"Bubbles" thats a funny word!

:getstoned:


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OfflineWizzy
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Re: Agar broth, throw out your crap LCs [Re: ShadOWCrack]
    #13781767 - 01/13/11 05:20 PM (13 years, 17 days ago)

Quote:

longslocksmith said:
Ohhhh i thought i fucked up when i did my first set of agar jars, it had bubbles 
"Bubbles" thats a funny word!

:getstoned:




LOL "La Vida Loca"


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InvisibleTranscendingLife
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Re: Agar broth, throw out your crap LCs [Re: Wizzy]
    #13781835 - 01/13/11 05:30 PM (13 years, 17 days ago)

About time...jeez:lmafo:
Love it JF!

Here's mine:


Between 2 jars it was 500mL water 10g Extra Light Malt Extract + 2.3 g Agar-Agar. I feel it's  bit heavy on the agar side. Next time I'll be using 1.7-1.8 g Agar...I'll let ya know how that turns out.:greenthumb:


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