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OfflineTheCannabisSativa
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Does alcoholl kill trich spores?
    #13776238 - 01/12/11 05:46 PM (13 years, 18 days ago)

A couple weeks ago my grow got contaminated after I tried to save a cake by cutting out a green spot.

I'm worried because I hear this can ruin future grow attempts as the spores stick to everything.....

I have 7 cakes set to be birthed on Saturday but before that I plan on filling a spray bottle with 91% alcohol and lightly misting every surface in the room...ceiling, walls, floor, furniture, ect.

Will this kill trich spores or would it be a waste of my time?


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Offlinedamnyoumongorians
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Re: Does alcoholl kill trich spores? [Re: TheCannabisSativa]
    #13776300 - 01/12/11 05:58 PM (13 years, 18 days ago)

how bout you use bleach...


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OfflineTheCannabisSativa
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Re: Does alcoholl kill trich spores? [Re: damnyoumongorians]
    #13776340 - 01/12/11 06:06 PM (13 years, 18 days ago)

would a bleach spray be better?  if it is I'll def use it.

was just thinking how I wouldn't have to worry about bleaching the color out of any fabrics when I spray the alcohol but I guess a bleach mist shouldn't do much damage......


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Offlinediatom
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Re: Does alcoholl kill trich spores? [Re: damnyoumongorians]
    #13776349 - 01/12/11 06:06 PM (13 years, 18 days ago)

I would use bleach, spraying a flammable liquid seems like a bad idea.


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Offlineafrosheen
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Re: Does alcoholl kill trich spores? [Re: diatom]
    #13776353 - 01/12/11 06:07 PM (13 years, 18 days ago)

Trich spores are everywhere, just be very careful with your sterile tech and you'll be fine.


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Offlinedamnyoumongorians
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Re: Does alcoholl kill trich spores? [Re: TheCannabisSativa]
    #13776356 - 01/12/11 06:08 PM (13 years, 18 days ago)

cuz im pretty sure alcohol needs friction for it to work...and it would probably evaporate before you could do wipe it down at 91%


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OfflineTheCannabisSativa
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Re: Does alcoholl kill trich spores? [Re: afrosheen]
    #13776383 - 01/12/11 06:16 PM (13 years, 18 days ago)

I was fine innoculating jars in the room after the incident and kind of in open air (I put the needle in the syringe in a glove box but its to short to innculate in)  So I just take the top off so its open air but I figure the sides help keep air movement down.....out of 11 jars inoculated  0 got contaminated so maybe I'm not in to bad of shape.....

I just want to kill as many spores as I can before I start fanning my FC.

and yea good call with the flammable spraying.....I'll be using bleach, thanks for the help.


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OfflineRogerRabbitM
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Re: Does alcoholl kill trich spores? [Re: TheCannabisSativa] * 1
    #13776559 - 01/12/11 06:46 PM (13 years, 18 days ago)

Use a 1:10 ratio of laundry bleach to water.  After mixing, add the same amount of vinegar that you used in bleach.  Spray this into the air and on all the surfaces.  It's not a cure for having released billions of trich spores into the air, but it's about all you can do.
RR


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Invisibleandymc
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Re: Does alcoholl kill trich spores? [Re: RogerRabbit]
    #13776582 - 01/12/11 06:49 PM (13 years, 18 days ago)

:imwithstupid:

Also no, alcohol doesn't kill spores.


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OfflineTheCannabisSativa
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Re: Does alcoholl kill trich spores? [Re: andymc]
    #13776786 - 01/12/11 07:20 PM (13 years, 18 days ago)

Thanks for the advice.....very much appreciated!  and I will do exacly that, hope for the best, and always toss contaminated cakes on site immediatly in the future!


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OfflineDisgruntled
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Re: Does alcoholl kill trich spores? [Re: TheCannabisSativa]
    #13777058 - 01/12/11 08:13 PM (13 years, 18 days ago)

yeah,. rubbing alcohol does not sterilize,. its used to sanatize,.. huge difference,. and as someone said,.  above,. you have to use friction for it to work,. hence the name,. "rubbing" alcohol. alot of people that get contams,. prob happen because tey flame a needle, and then wipe that "flame sterile" needle off with an alcohol soaked paper towel,. which makes it unsterile and sanatized,.


bleach/vinegar would be your best bet,.. would be really nice to run a hepa in the room for a good while aswell


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Offlinehamloaf
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Re: Does alcoholl kill trich spores? [Re: TheCannabisSativa]
    #13778845 - 01/13/11 06:12 AM (13 years, 17 days ago)

Before the trichoderma turns green and when it arrives on the surface is the only time you can safely practice holding trichoderma back with chemical treatments.
Used the salt paste to contain trichoderma once it's appeared on the surface.
Only when substrate was just a few days from fruiting and never when green is detected.
When the green has arrived, it's too late, your room has become infected.

When infection grows from the middle of the substrate, it's usually due to bad grain spawn.
This infection spreads through out the middle of the entire substrate very rapidly and is untreatable.
To reiterate, rubbing alcohol doesn't kill trichoderma spores, it spreads them.
The only cure from an infected room due to trichoderma sporulation is deep clean of your fruiting area before introducing any more fruiting substrates into it.


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OfflineTheCannabisSativa
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Re: Does alcoholl kill trich spores? [Re: hamloaf]
    #13780608 - 01/13/11 02:07 PM (13 years, 17 days ago)

I know I'm a little late but if anyones reading....the 11 jars I have coliniziong - is it safe to spray that bleach/vinegar/water mix around them with the dry verm layer n all or should I place some cardboard or something over the top of them when I spray?

Also I think I'll do it today and then again Saturday hours before birthing just to do the best job I could....


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Offlinedamnyoumongorians
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Re: Does alcoholl kill trich spores? [Re: TheCannabisSativa]
    #13780837 - 01/13/11 02:49 PM (13 years, 17 days ago)

i would move them just to be safe.just dont shake them or anything of that nature.


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OfflineDisgruntled
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Re: Does alcoholl kill trich spores? [Re: damnyoumongorians]
    #13783131 - 01/13/11 09:36 PM (13 years, 17 days ago)

they would be fine as is,. if you are worried about contam, youd be more likely to contam them from moving the jars and having the verm shift,. then you would be with having the cleaning spray misted around them,.

if you have a bad problem with contams,. and you do/are moving them,. just be really careful not to shake/bump them,. try to hold them pretty level and move slowly and gently while moving and picking up or setting down,. but,. youd be better off, with your hands off if your contam issues are petty bad,. IMO

good luck


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OfflineDisgruntled
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Re: Does alcoholl kill trich spores? [Re: Disgruntled]
    #13783145 - 01/13/11 09:40 PM (13 years, 17 days ago)

ill have to do some quick research, but i THINK, am not sure,. but if i remember right, i believe ozone (o3) from an ozone generator will kill the mold/trich spores. im either really lucky, or it does kill the shit out of stuff, as ive been use'n one i built and havent had contam issues, and ive been pretty brave and have done open air G2G and agar work in my grow room,. i also run a decent sized hepa most of the day, which could be whats saving my bacon,. ill do some research and post my findings,.

but if memory serves correct the o3 will oxidize the spores and render them inert, on the same token though, you have to be mindful cause it will do the same to your shroom spores while your spore printing if it comes on,.

ill postback soon


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Invisibleanonjon
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Re: Does alcoholl kill trich spores? [Re: Disgruntled]
    #13783386 - 01/13/11 10:22 PM (13 years, 17 days ago)

You can sterilize surfaces using a combination of h202 and vinegar. You make up a spray bottle of each, hit it with the vinegar, then hit it with the h202.

This is much more effective than bleach or alcohol and a lot more practical than an ozone generator.

The threat of ambient spores during fruiting is highly exaggerated btw.


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OfflineDisgruntled
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Re: Does alcoholl kill trich spores? [Re: anonjon]
    #13783486 - 01/13/11 10:34 PM (13 years, 17 days ago)

yeah i hear that on the spores while fruiting,.

the ozone generators a actually prett simple to build,. the reason i built mine is it eleminates odors and was used while a friend of a friends friend was growing some herbage to kill the smell.

and yes alcohol=:laugh2: for the most part,


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OfflineTheCannabisSativa
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Re: Does alcoholl kill trich spores? [Re: Disgruntled]
    #13784483 - 01/14/11 02:58 AM (13 years, 16 days ago)

if you look up a few posts you'll see a very trusted member recomended I used a bleach/vinegar solution and the only reason I'm doing all this is because I had contamination on grow attempt 1, I'm not just doing it for the hell of it be extra cautious or anything like that.

Thanks to everybody for the input


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Invisibleanonjon
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Re: Does alcoholl kill trich spores? [Re: TheCannabisSativa]
    #13790503 - 01/15/11 10:20 AM (13 years, 15 days ago)

Quote:

TheCannabisSativa said:
if you look up a few posts you'll see a very trusted member recomended I used a bleach/vinegar solution and the only reason I'm doing all this is because I had contamination on grow attempt 1, I'm not just doing it for the hell of it be extra cautious or anything like that.

Thanks to everybody for the input




You've got it wrong. It's h202 and vinegar.


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