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m00nshine
ÜBER SHAMAN




Registered: 11/18/05
Posts: 9,774
Loc: BALLS DEEP
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Dry, Stalled WBS Jars? 1
#13717304 - 01/01/11 11:06 PM (14 years, 18 days ago) |
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Introduction:
This is a very easy technique to get dry, stalling seed jars to finish colonizing. If the jars aren't prepared perfectly, sometimes they will dry out and stall. I've only heard of contams and other problems causing stalling, but with a little experimentation I've figured out that a low moisture content can cause this, too.
Procedure:
1. Boil some water in a pot for 15-20 minutes. 2. Turn flame / burner off and let the water sit for 5 minutes or so. 3. Suck up the water into a sterile syringe. 4. Allow syringe to cool overnight. 5. Flame sterilize needle. Inject dry half pints with ~2.5cc water. For quarts, use about 10cc. 6. Place micro pore tape over injection site. 7. Shake jar vigorously, it doesn't matter if you've already shaken the jar to distribute colonized grains.
And that's it! Jars will bounce back within 2 days. The rate of colonization will speed up significantly. I figured this would help a few people out while getting the hang of correctly preparing WBS, GL.
Pictures:
Jars that had dried out after the shake, due to skipping the simmering step of WBS prep:

This is right before injection, an "after" pic will be provided within the next few days, they are doing really well. That is a 60cc syringe in the picture.
Jars that had dried out rather quickly, due to too little simmering time (~15 minutes, very low heat):

After injecting 2cc water; I say 2.5cc above because 2cc was barely enough to have it finish:
Edited by m00nshine (01/11/11 10:46 AM)
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runMD
Face Thoroughly Palmed


Registered: 11/21/10
Posts: 955
Loc: South Park County, CO
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Re: Dry, Stalled WBS Jars? [Re: m00nshine]
#13717322 - 01/01/11 11:11 PM (14 years, 18 days ago) |
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I noticed this in your growlog. Nice to see a tek putting it in the open.
Will try if I have a stalled jar. How long do you usually wait before you consider a jar to be stalled if using WBS inoculated with multispore. What about liquid culture?
-------------------- "Are you high or just stupid?"
"I assure you, I am NOT high"
::All my posts are fabrications or observed accounts of other people's experiences. All cultivation is for the growth of legal and edible gourmet mushrooms.::
Not A Medical Doctor.
Elementary Coir Tek
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Study Them. Experience Them.Learn from Them. Respect Them.
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m00nshine
ÜBER SHAMAN




Registered: 11/18/05
Posts: 9,774
Loc: BALLS DEEP
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Re: Dry, Stalled WBS Jars? [Re: runMD]
#13717341 - 01/01/11 11:15 PM (14 years, 18 days ago) |
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If I don't see any growth in 4 - 7 days at optimal temps, I consider it stalled. You will be able to tell that the seed is dry, also.
If you shake and notice that it has stopped colonizing, and that there's a big chunk of myc in the middle of the jar, it has stalled. Especially if the chunk is pretty solid with no noticeable colonization of nearby loose grains.
I don't use LC
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runMD
Face Thoroughly Palmed


Registered: 11/21/10
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Loc: South Park County, CO
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Re: Dry, Stalled WBS Jars? [Re: m00nshine]
#13717348 - 01/01/11 11:16 PM (14 years, 18 days ago) |
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Not to go on a thread tangent...
Do you use G2G, Agar, or do you always go multispore?
-------------------- "Are you high or just stupid?"
"I assure you, I am NOT high"
::All my posts are fabrications or observed accounts of other people's experiences. All cultivation is for the growth of legal and edible gourmet mushrooms.::
Not A Medical Doctor.
Elementary Coir Tek
Damion's Favorite Links, I love em.
Study Them. Experience Them.Learn from Them. Respect Them.
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m00nshine
ÜBER SHAMAN




Registered: 11/18/05
Posts: 9,774
Loc: BALLS DEEP
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Re: Dry, Stalled WBS Jars? [Re: runMD]
#13717352 - 01/01/11 11:18 PM (14 years, 18 days ago) |
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MS all day, all the time. I don't have the time or ability to go any further at this stage... I do all my work in secret and have devised an ideal way of stealth growing for myself.
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runMD
Face Thoroughly Palmed


Registered: 11/21/10
Posts: 955
Loc: South Park County, CO
Last seen: 11 years, 2 months
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Re: Dry, Stalled WBS Jars? [Re: m00nshine]
#13717362 - 01/01/11 11:19 PM (14 years, 18 days ago) |
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Thanks M00N. Yea,I read your whole growlog and noticed they covert ops you had going. The twist about almost losing some jars when a roommate found them...I'm sorry, but I lol'd.
-------------------- "Are you high or just stupid?"
"I assure you, I am NOT high"
::All my posts are fabrications or observed accounts of other people's experiences. All cultivation is for the growth of legal and edible gourmet mushrooms.::
Not A Medical Doctor.
Elementary Coir Tek
Damion's Favorite Links, I love em.
Study Them. Experience Them.Learn from Them. Respect Them.
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m00nshine
ÜBER SHAMAN




Registered: 11/18/05
Posts: 9,774
Loc: BALLS DEEP
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Re: Dry, Stalled WBS Jars? [Re: runMD]
#13717366 - 01/01/11 11:21 PM (14 years, 18 days ago) |
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haha, it's cool, that was at SWIM's house, LOL
my stuff has never been seen by my roomies
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Dry, Stalled WBS Jars? [Re: m00nshine]
#13717372 - 01/01/11 11:22 PM (14 years, 18 days ago) |
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Do you get dry jars very often? I wonder if your grain prep needs some fine tuning, maybe soak longer?
I've had bad luckj adding water to jars, I'm glad it works for somebody. I've had better luck with dry jars by using them for g2g or just spawning the colonized parts to cioir. But that's pretty sketchy.
-------------------- You make it all possible. Doesn't it feel good?
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m00nshine
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Registered: 11/18/05
Posts: 9,774
Loc: BALLS DEEP
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Re: Dry, Stalled WBS Jars? [Re: Doc_T]
#13717386 - 01/01/11 11:27 PM (14 years, 18 days ago) |
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No, it only happens when I don't follow my current working method exactly. Basically when I try to cut corners: Not simmering, simmering for less time than usual, allowing the seed to dry for too long (or using paper towels to aid in drying). The procedure in my bulk growing guide is pretty spot on for good results. There's a few things that need to be updated, but the core technique has given me great success. 
I was going to spawn the colonized parts, but I wanted more available spawn.
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
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Re: Dry, Stalled WBS Jars? [Re: m00nshine]
#13718040 - 01/02/11 04:39 AM (14 years, 17 days ago) |
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How well do you think this would work with rye? My rye seems to have dried out (used RR's grain tek from the vid) it's been 3 weeks and they've been slowing down and dried out. GE is part of it, but due to slow growth... yeah... ideas?
-------------------- We think we have freedom, but we're all just mice in a maze....
FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!
I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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m00nshine
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Registered: 11/18/05
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I've never used rye, but if I did, I'd use the same amount of water
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m00nshine
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Registered: 11/18/05
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Re: Dry, Stalled WBS Jars? [Re: m00nshine]
#13736390 - 01/05/11 05:08 PM (14 years, 14 days ago) |
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See what can happen in 3 days? 
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literatestylish
Stranger


Registered: 05/25/10
Posts: 207
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Re: Dry, Stalled WBS Jars? [Re: m00nshine]
#13736498 - 01/05/11 05:26 PM (14 years, 14 days ago) |
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Wow.
RR says to only boil/simmer for only 10 minutes after the soak. Is that because Rye and WBS are different in simmering time?
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m00nshine
ÜBER SHAMAN




Registered: 11/18/05
Posts: 9,774
Loc: BALLS DEEP
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I don't know much about rye prep...
I simmer my WBS for about 40 minutes on medium heat.
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
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Quote:
literatestylish said: Wow.
RR says to only boil/simmer for only 10 minutes after the soak. Is that because Rye and WBS are different in simmering time?
The birdseed mix has smaller grains that'll easily become burst if you let the seed over-boil. It's been recommended in the past that birdseed be simmered because of the grain size of birdseed over rye.
For birdseed, RR's tek for rye preparation will safely prepare birdseed for loading into jars or bags. Be sure to stay right on top of the boil step.
Have a meat thermometer handy. Once the water reaches 200F, start a timer if you have to for ten minutes. It's critical that you not let the birdseed boil for longer than ten minutes. Boiling the birdseed for longer than 10 minutes when using RR's rye prep increases your chance for busted kernels greatly.
Following RR's rye prep for birdseed, allot for a longer time for your birdseed to become properly hydrated over rye before loading into jars or bags.
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
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Re: Dry, Stalled WBS Jars? [Re: hamloaf]
#13737736 - 01/05/11 08:44 PM (14 years, 14 days ago) |
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the water has kinda helped already, half the jars it wasn't enough water... I'll probably use 20cc per qt of rye in 4 more days to keep the myc going
-------------------- We think we have freedom, but we're all just mice in a maze....
FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!
I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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m00nshine
ÜBER SHAMAN




Registered: 11/18/05
Posts: 9,774
Loc: BALLS DEEP
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Are you sure it wasn't enough? I'd say let it go for longer, perhaps a week, before injecting again, if at all. You don't want to keep moving your jars around. I've known people who have handled jars frequently and they would contaminate. I'm sure other factors like the environment were involved.. It could have been a dirty place but I'm fairly certain most of it was due to handling too often.
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PuffinChronic
Phat Pockets



Registered: 12/27/10
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Loc: West Coast Canada
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Re: Dry, Stalled WBS Jars? [Re: m00nshine]
#13738228 - 01/05/11 10:01 PM (14 years, 14 days ago) |
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Im still new to the hobby but all I have delt with is WBS and G2G transfers... I still have yet to encounter contamination *fingers crossed* When I prepared the WBS for the first time I read to simmer for 1 hour after the 24 hr rinse/soak period but when I got to the 30 min mark I took a grain and it wasn't easy to squish between my fingers and it wasn't hard either. I turned the stove off and havent looked back ever since! goin on my 3rd transfer and so far Im laughin!!
-------------------- “Live. Let nothing be lost upon you. Be afraid of nothing.”
- George Sanders. The Picture of Dorian Gray 1945
 
Edited by PuffinChronic (01/06/11 06:10 PM)
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slapphappypill
Enthusiast!




Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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Yeah, I was doing the 10 min simmer. I'll be going to 30 mins from now on... I'll wait until monday before I mess with them then... but I'm 90% sure I'l be adding 10 more CCs of water for 80% of the jars right now... they still just look way too dry
-------------------- We think we have freedom, but we're all just mice in a maze....
FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!
I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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rusty_blade

Registered: 10/13/10
Posts: 13
Loc: mid-west
Last seen: 4 years, 2 months
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Hey Moonshine thanks for the info! I just did my first four jars of WBS and got the water content wrong. They had been stalled out at about 15% for like 2 weeks! I added 10cc's of water and in three days they are around 90%!!! THANKS DUDE!!! i thought i was fucked.
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