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Invisiblearis
Feline Bovine


Registered: 07/20/10
Posts: 715
Loc: dl
Day 14 Waiting 4 Gemination from 2 old prints on 2/3 strength agar.
    #13714049 - 01/01/11 06:49 AM (13 years, 29 days ago)



0ff to slow start
Day 7 all empty dishes poured still visibly appearing contaminant free.
Dishes started a week prior contaminated by old syringe vector and destroyed.

Waited a day and started over.

Inoc loop to 3 dishes with 1 print and made new syringe with 2nd print.  Immeadiately afterward inoc 3 more dish.

Day 21.
Waiting for gemination from the 2 OLD prints on 2/3 strength agar.

No contamination but the anticipation is going to give me an anurism.. I hate waiting only to find I did something wrong and i should of tried again 14 days ago.

Maybe will start 3 more dished today with hydrated spored in 2 week old syringe.

Im worried I over did it with the 10% bleach water in the GB or too much parafilm and not enuf air?  I hate bleach water fumes...hurts lungs (want flo)

Sick of waiting so I raised the temp from 70 to 77.

Could I have ruined a print by spraying the outside of its foil with lysol?


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OfflineRogerRabbitM
Bans for Pleasure
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 2 days
Trusted Cultivator
OG Cultivator
Re: Day 14 Waiting 4 Gemination from 2 old prints on 2/3 strength agar. [Re: aris]
    #13714280 - 01/01/11 09:32 AM (13 years, 29 days ago)

Try placing a drop of sterilized water over the spores in each dish.  They probably need hydration.  Lysol fumes will kill spores, so if they got inside, that could be an issue.

Also, despite what you'll read here elsewhere, it's very hard to get spores over three to four years old to germinate.  Above five years, many spores are dead and won't germinate no matter  what you do.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Invisiblearis
Feline Bovine


Registered: 07/20/10
Posts: 715
Loc: dl
Re: Day 14 Waiting 4 Gemination from 2 old prints on 2/3 strength agar. [Re: RogerRabbit]
    #13714418 - 01/01/11 10:32 AM (13 years, 29 days ago)

Will try...


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