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OfflineJohnny Springfield
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How do you guys quickly go from isolate to PF cakes for testing?
    #13704243 - 12/30/10 01:51 AM (13 years, 1 month ago)

I've read it said by a few people that PF cakes are the quickest way to test if an isolate has the traits you want.

But what's the quickest way to go from an isolate on agar to PF cakes?

I realize you could take a slice of the isolate and make a grain LC to knock up some PF jars, but is that how most of you do it?

Is there another way that's faster that I'm missing?


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Invisiblejokefox
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Re: How do you guys quickly go from isolate to PF cakes for testing? [Re: Johnny Springfield]
    #13704261 - 12/30/10 02:00 AM (13 years, 1 month ago)

either cake
or bulk

if im lazy or gutsy has to do alot with it


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OfflineJohnny Springfield
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Re: How do you guys quickly go from isolate to PF cakes for testing? [Re: jokefox]
    #13704331 - 12/30/10 02:31 AM (13 years, 1 month ago)

Quote:

jokefox said:
either cake
or bulk

if im lazy or gutsy has to do alot with it




But how do you go from the isolate on agar to those?  Is knocking up a grain jar with an agar wedge the only way?


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Invisibleiamnotadream
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Re: How do you guys quickly go from isolate to PF cakes for testing? [Re: Johnny Springfield]
    #13704342 - 12/30/10 02:35 AM (13 years, 1 month ago)

you -could- simply exclude the vermiculite layer in your pf jars, giving additional space to stick your wedge in.

making lc/putting the wedge in grains is more ideal, i think.


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OfflineNaz
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Re: How do you guys quickly go from isolate to PF cakes for testing? [Re: iamnotadream]
    #13704455 - 12/30/10 03:45 AM (13 years, 1 month ago)

just do one pint jar of grain and a very small dish for sub.... "quick" seems almost the stuff of jokes in this hobby IMO....


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OfflineJohnny Springfield
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Re: How do you guys quickly go from isolate to PF cakes for testing? [Re: Naz]
    #13704709 - 12/30/10 07:14 AM (13 years, 1 month ago)

Quote:

Naz said:
just do one pint jar of grain and a very small dish for sub.... "quick" seems almost the stuff of jokes in this hobby IMO....




Well, yeah, I mean "quick" relatively speaking. :smile:

I know everything in this takes patience.  I just thought there might be another method to go straight to cakes or something, but I wasn't seeing it.


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InvisibleDoc_T
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Re: How do you guys quickly go from isolate to PF cakes for testing? [Re: Johnny Springfield]
    #13704803 - 12/30/10 07:41 AM (13 years, 1 month ago)

I do agar->lc or agar->grain->grain lc.

The step from agar to cake is the slow step of the process, the bottleneck.


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Invisibleanonjon
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Re: How do you guys quickly go from isolate to PF cakes for testing? [Re: Doc_T]
    #13704828 - 12/30/10 07:49 AM (13 years, 1 month ago)

You don't go quickly cuz there's no need to test agar. You're testing the LC. Can't test it till it's been made.

That's why when I receive a syringe the first thing I do is inoculate LC's with it and worry about isolation or cloning later.


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OfflineJohnny Springfield
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Re: How do you guys quickly go from isolate to PF cakes for testing? [Re: Doc_T]
    #13704835 - 12/30/10 07:54 AM (13 years, 1 month ago)

Quote:

Doc_T said:
I do agar->lc or agar->grain->grain lc.

The step from agar to cake is the slow step of the process, the bottleneck.




That's pretty much what I thought, but I was hoping I was missing something here.

Thanks. :smile:


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OfflineRogerRabbitM
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Re: How do you guys quickly go from isolate to PF cakes for testing? [Re: Johnny Springfield]
    #13705010 - 12/30/10 09:20 AM (13 years, 1 month ago)

The fastest method of testing isolates is to make brf cakes without the verm layer, using a tyvek filter instead.  Place a wedge of each strain in each brf cake.  Don't waste time making LC(ever) or going to grains if the species you're testing will grow on cakes.
RR


--------------------
Download Let's Grow Mushrooms



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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineJohnny Springfield
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Re: How do you guys quickly go from isolate to PF cakes for testing? [Re: RogerRabbit]
    #13705113 - 12/30/10 09:52 AM (13 years, 1 month ago)

Quote:

RogerRabbit said:
The fastest method of testing isolates is to make brf cakes without the verm layer, using a tyvek filter instead.  Place a wedge of each strain in each brf cake.  Don't waste time making LC(ever) or going to grains if the species you're testing will grow on cakes.
RR




Awesome.  I will try this. :smile:


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Invisibleanonjon
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Re: How do you guys quickly go from isolate to PF cakes for testing? *DELETED* [Re: RogerRabbit]
    #13705144 - 12/30/10 10:00 AM (13 years, 1 month ago)

Post deleted by RogerRabbit

Reason for deletion: Discussion of weights and bulk growing is off topic in a thread about strain isolation and quick testing of individual strains.



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The above post is fictional, hypothetical, or downright nonsensical.


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InvisibleLeopardMan
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Re: How do you guys quickly go from isolate to PF cakes for testing? *DELETED* [Re: anonjon]
    #13705195 - 12/30/10 10:15 AM (13 years, 1 month ago)

Post deleted by RogerRabbit

Reason for deletion: Discussion of weights and bulk growing is off topic in a thread about strain isolation and quick testing of individual strains.



--------------------



You have to die a few times before you can really live.

-Charles Bukowski-


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Invisibleanonjon
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Re: How do you guys quickly go from isolate to PF cakes for testing? *DELETED* [Re: LeopardMan]
    #13705397 - 12/30/10 11:07 AM (13 years, 1 month ago)

Post deleted by RogerRabbit

Reason for deletion: Discussion of weights and bulk growing is off topic in a thread about strain isolation and quick testing of individual strains.



--------------------
The above post is fictional, hypothetical, or downright nonsensical.


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InvisibleDoc_T
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Re: How do you guys quickly go from isolate to PF cakes for testing? *DELETED* [Re: anonjon]
    #13705406 - 12/30/10 11:09 AM (13 years, 1 month ago)

Post deleted by RogerRabbit

Reason for deletion: Discussion of weights and bulk growing is off topic in a thread about strain isolation and quick testing of individual strains.



--------------------
You make it all possible. Doesn't it feel good?


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Invisibleanonjon
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Re: How do you guys quickly go from isolate to PF cakes for testing? *DELETED* [Re: Doc_T]
    #13705475 - 12/30/10 11:25 AM (13 years, 1 month ago)

Post deleted by RogerRabbit

Reason for deletion: Discussion of weights and bulk growing is off topic in a thread about strain isolation and quick testing of individual strains.



--------------------
The above post is fictional, hypothetical, or downright nonsensical.


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Edited by anonjon (12/30/10 11:26 AM)


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InvisibleLeopardMan
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Re: How do you guys quickly go from isolate to PF cakes for testing? *DELETED* [Re: anonjon]
    #13705537 - 12/30/10 11:45 AM (13 years, 1 month ago)

Post deleted by RogerRabbit

Reason for deletion: Discussion of weights and bulk growing is off topic in a thread about strain isolation and quick testing of individual strains.



--------------------



You have to die a few times before you can really live.

-Charles Bukowski-


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Invisibleanonjon
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Re: How do you guys quickly go from isolate to PF cakes for testing? *DELETED* [Re: LeopardMan]
    #13705558 - 12/30/10 11:50 AM (13 years, 1 month ago)

oops.


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Edited by anonjon (12/30/10 12:38 PM)


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OfflineRogerRabbitM
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Re: How do you guys quickly go from isolate to PF cakes for testing? *DELETED* [Re: anonjon]
    #13705744 - 12/30/10 12:27 PM (13 years, 1 month ago)

Stay on topic please.  It's a thread about testing isolates.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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InvisibleDoc_T
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Re: How do you guys quickly go from isolate to PF cakes for testing? *DELETED* [Re: RogerRabbit]
    #13705867 - 12/30/10 12:54 PM (13 years, 1 month ago)

Sorry, I got caught up in the math. :blush:


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